Methylation is maintained specifically at imprinting control regions but not other DMRs associated with imprinted genes in mice bearing a mutation in the Dnmt1 intrinsically disordered domain.

Differential methylation of imprinting control regions in mammals is essential for distinguishing the parental alleles from each other and regulating their expression accordingly. To ensure parent of origin-specific expression of imprinted genes and thereby normal developmental progression, the differentially methylated states that are inherited at fertilization must be stably maintained by DNA methyltransferase 1 throughout subsequent somatic cell division. Further epigenetic modifications, such as the acquisition of secondary regions of differential methylation, are dependent on the methylation status of imprinting control regions and are important for achieving the monoallelic expression of imprinted genes, but little is known about how imprinting control regions direct the acquisition and maintenance of methylation at these secondary sites. Recent analysis has identified mutations that reduce DNA methyltransferase 1 fidelity at some genomic sequences but not at others, suggesting that it may function differently at different loci. We examined the impact of the mutant DNA methyltransferase 1 P allele on methylation at imprinting control regions as well as at secondary differentially methylated regions and non-imprinted sequences. We found that while the P allele results in a major reduction in DNA methylation levels across the mouse genome, methylation is specifically maintained at imprinting control regions but not at their corresponding secondary DMRs. This result suggests that DNA methyltransferase 1 may work differently at imprinting control regions or that there is an alternate mechanism for maintaining methylation at these critical regulatory regions and that maintenance of methylation at secondary DMRs is not solely dependent on the methylation status of the ICR.

The Value and Validity of Self-Reported Survey Data on the Rape Experiences of College Students.

Self-reported survey data on the extent and nature of rape and sexual assault experienced by a population represent an important source of information because these crimes often go unreported, and are thus undercounted in law enforcement or other official statistics. This article compares Campus Climate Survey Validation Study (CCSVS) data to Clery Act data in an effort to (1) assess the validity of the CCSVS data and the Clery Act data based on the extent to which they corroborate one another, and (2) estimate the extent to which Clery Act data potentially underestimate the true incidence of rape. The results help to establish the extent to which self-report surveys on sexual victimization are needed to understand the magnitude of the problem among a given population.

Prevention of sexual violence among college students: Current challenges and future directions.

Objective Preventing sexual violence among college students is a public health priority. This paper was catalyzed by a summit convened in 2018 to review the state of the science on campus sexual violence prevention. We summarize key risk and vulnerability factors and campus-based interventions, and provide directions for future research pertaining to campus sexual violence. Results and Conclusions: Although studies have identified risk factors for campus sexual violence, longitudinal research is needed to examine time-varying risk factors across social ecological levels (individual, relationship, campus context/broader community and culture) and data are particularly needed to identify protective factors. In terms of prevention, promising individual and relational level interventions exist, including active bystander, resistance, and gender transformative approaches; however, further evidence-based interventions are needed, particularly at the community-level, with attention to vulnerability factors and inclusion for marginalized students.

Sensitivity of Sexual Victimization Estimates to Definitional and Measurement Decisions.

Self-report surveys are subject to measurement error associated with variation in the methodology employed. The current analysis uses data from the Campus Climate Survey Validation Study (CCSVS) to examine the impact that measurement decisions have on estimates. The findings demonstrate that asking victims to provide detailed information in an effort to properly place incidents in time and classify incidents by type resulted in relatively minor decreases in estimate magnitude. Ultimately, asking respondents to provide or confirm additional incident-level information for proper classification resulted in more complete information with very little impact on estimates.

Methods for Improving Representativeness in a Web Survey on Sexual Assault Among College Students.

Many colleges and universities conduct web-based campus climate surveys to understand the prevalence and nature of sexual assault among their students. When designing and fielding a web survey to measure a sensitive topic like sexual assault, methodological decisions, including the length of the field period and the use or amount of an incentive, can affect the representativeness of the respondent sample leading to biased or imprecise estimates. This study uses data from the Campus Climate Survey Validation Study (CCSVS) to assess how the interaction between field period length and survey incentive amount affects nonresponse, sample representativeness, and the precision of survey estimates. Research suggests that using robust incentives gives potential survey respondents a reason to complete the survey beyond their intrinsic motivation to do so. Likewise, extending the field period gives more time to people who may be less intrinsically motivated to complete the survey. Both serve to increase sample size and representativeness, minimize bias, and improve estimate precision. Schools, however, sometimes lack the time and/or resources for both a robust incentive and a lengthy field period, and this study examines the extent to which the potential negative impacts of not using one can be mitigated by the presence of the other. Findings indicate that target response rates can be achieved using a smaller incentive if the field period is lengthy but, even with a lengthy field period, the use of a smaller incentive can result in biased estimates due to a lack of representativeness. Conversely, when a robust incentive is used and weights are developed to adjust for nonresponse, a shorter field period will not have a significant impact on point estimates, but the estimates will be less precise due to fewer respondents participating in the survey.

Understanding (and Acting On) 20 Years of Research on Violence and LGBTQ + Communities.

Questions related to violence, vulnerability, and sexual and gender minorities continuing to occupy a focal place in U.S. public discourse. We reviewed findings from 20 years of research on that topic to make recommendations for policy, practice, and future research. This article synthesizes findings from 102 peer-reviewed articles as well as a small number of unpublished studies and grey literature. We found no data to support the idea (widespread in popular discourse) that those in the sexual or gender majority require protection from sexual or gender minority individuals. Instead, this wide body of research indicates that sexual and gender minorities are themselves at elevated risk for physical and sexual assault, harassment, bullying, and hate crime victimization throughout childhood, adolescence, and adulthood. Contradicting the image of hate crimes as perpetrated by strangers or acquaintances, we find that bias-related verbal abuse, physical, and sexual assault by close family members contribute heavily to observed victimization rates. Further, despite the perception that society is becoming more welcoming, victimization disparities appear to be stable or widening since the 1990s. More studies with probabilistic sampling approaches, standardized measures, and larger samples of gender minorities are needed. However, widespread victimization of sexual and gender minorities is clearly an urgent issue, demanding attention from clinicians, program developers, and policy makers.

Disclosure of sexual assault experiences among undergraduate women at historically black colleges and universities (HBCUs).

OBJECTIVE: To document the sexual assault disclosure experiences of historically black college or university (HBCU) students. PARTICIPANTS: A total of 3,951 female, undergraduate students at 4 HBCUs. METHODS: All women at the participating schools were recruited in November 2008 to participate in a Web-based survey including both closed- and open-ended questions. Survey data were weighted for nonresponse bias. RESULTS: The majority of sexual assault survivors disclosed their experience to someone close to them, but disclosure to formal supports, particularly law enforcement agencies, was extremely rare. Nonreporters had concerns about the seriousness of the incident and their privacy. On the basis of qualitative data, strategies identified by students to increase reporting included more education and awareness about sexual assault, more survivor services and alternative mechanisms for reporting, and better strategies for protecting the confidentiality of survivors. CONCLUSIONS: Official sexual assault victimization data are of limited utility in conveying the extent of sexual assault among HBCU students, and efforts to increase reporting, such as peer education and enhanced confidentiality procedures, are needed.

Intimate partner violence victimization among undergraduate women at Historically Black Colleges and Universities (HBCUs).

Despite the evidence that young and minority women may be particularly vulnerable to intimate partner violence (IPV), there is little research on the IPV experiences of minority undergraduate women. This study addresses this gap by estimating the prevalence of IPV and examining factors associated with experiencing IPV among undergraduate women attending Historically Black Colleges or Universities (HBCUs). Findings suggest alarmingly high victimization rates; however, factors associated with IPV among HBCU women are similar to those found in prior research with women in the general population. The results also suggest that some risk factors are differentially associated with experiencing specific types of IPV.

The context and consequences of sexual assault among undergraduate women at Historically Black Colleges and Universities (HBCUs).

To examine the context of sexual assault and postassault actions and consequences among women attending historically Black colleges or universities (HBCUs), web-based surveys were administered in November 2008 to 3,951 undergraduate women attending four HBCUs. Data on the context in which assaults occurred were generated for women who had been sexually assaulted since entering college (n = 358). Multivariate models were run on the full sample to examine the association between sexual assault and symptoms of depression and posttraumatic stress disorder (PTSD). Results indicated that most survivors were assaulted by assailants well known to them and when the survivor and perpetrator were drinking alcohol. Very few survivors disclosed their experiences to formal sources of support. Survivors had significantly more symptoms of depression and were more likely to screen positive for PTSD than nonvictims. Further research on disclosure and its moderating role on the mental health consequences of sexual assault is needed.

Approaches used by employee assistance programs to address perpetration of intimate partner violence.

Employee Assistance Programs (EAPs) are workplace resources available to employees with problems impacting work performance. EAPs are well-positioned to address intimate partner violence (IPV), a major public health problem with workplace impacts. A purposeful sample of 28 EAPs across the United States was surveyed to identify policies and programs to address IPV, including perpetration. Most EAPs did not report having standardized approaches for addressing IPV perpetration. EAPs also described significant barriers to identifying IPV perpetrators, with the majority relying on self-disclosure on the part of the perpetrator when contacting the EAP. These results suggest that many EAPs--even when interacting with employees who present with issues known to correlate with IPV--are missing a potential opportunity to assess and intervene with IPV perpetrators.

The sexual assault of undergraduate women at historically Black colleges and universities (HBCUs).

Although research has shown that undergraduate women are at high risk for experiencing sexual assault, little research has been conducted with undergraduate women who are attending a historically Black college or university (HBCU). The purpose of this research is to document the prevalence of different types of sexual assault among undergraduate women at HBCUs and make comparisons to data collected from undergraduate women at non-HBCUs. Data on sexual assault victimization were collected from 3,951 undergraduate women at HBCUs using a cross-sectional, web-based survey. These data are compared to data collected from 5,446 undergraduate women at non-HBCUs using the same research methods. Findings indicate that approximately 9.7% of undergraduate women at HBCUs report experiencing a completed sexual assault since entering college. This rate is considerably lower than the comparable rate obtained from undergraduate women at non-HBCUs (13.7%). This difference seems to be associated with differences in alcohol-use frequency. Perhaps undergraduate women at HBCUs drink alcohol much less frequently and are thus less likely to be sexually assaulted when they are incapacitated and unable to provide consent. Alcohol use frequency, while controlling for other factors, seems to have an independent association with the likelihood of an undergraduate woman being sexually assaulted. Implications for the creation and delivery of sexual assault risk reduction and prevention policies and programs are discussed.

Women's sexual orientations and their experiences of sexual assault before and during university.

PURPOSE: We sought to examine relationships between women's sexual orientations and their sexual assault experiences before and during university. METHODS: Self-reported responses on a web-based survey of 5,439 female undergraduates who participated in the Campus Sexual Assault study were analyzed to compare three groups: bisexuals, lesbians, and heterosexuals. Groups were compared in terms of the prevalence of sexual assault before and during university, and the extent to which sexual assault before university predicted sexual assault during university. FINDINGS: The prevalence of sexual assault before and during university was higher among bisexuals and lesbians compared with heterosexuals (25.4% of bisexuals, 22.4% of lesbians, and 10.7% of heterosexuals were sexually assaulted before university; 24.0% of bisexuals, 17.9% of lesbians, and 13.3% of heterosexuals were sexually assaulted during university). Sexual assault before university was highly predictive of sexual assault during university, especially among non-heterosexuals. Compared with heterosexuals not sexually assaulted before university (the referent group), previously assaulted non-heterosexuals (bisexuals/lesbians) had eight times the odds of sexual assault during university (adjusted odds ratio [AOR] = 8.75), whereas previously assaulted heterosexuals had four times the odds of sexual assault during university (AOR, 4.40). However, there was no difference in the odds of sexual assault during university between non-heterosexuals not sexually assaulted before university and heterosexuals not sexually assaulted before university. CONCLUSION: Bisexual and lesbian women are more likely than heterosexual women to be sexually assaulted before and during university. Sexual assault before university is linked to sexual assault during university for all women, with this association being especially pronounced among non-heterosexuals.

Comparing sexual assault prevalence estimates obtained with direct and indirect questioning techniques.

Concerns have been expressed about the validity of self-reported data on sexual assault, as victims might be reluctant to disclose what happened to them. In this study, using an anonymous, web-based survey, a sample of 5,446 undergraduate women were asked about their experiences with physically forced sexual assault using both direct and indirect questioning methods. The prevalence of physically forced sexual assault obtained via indirect questioning was slightly higher than, though not substantially or statistically different from, the estimate obtained via direct questioning. The results suggest that either direct questioning yields reasonably valid estimates of the prevalence of sexual assault or that the item count technique does not produce estimates that are any more valid.

Employee assistance program services for intimate partner violence and client satisfaction with these services.

OBJECTIVE: To describe intimate partner violence (IPV) services available through employee assistance programs (EAPs) and determine women's satisfaction with these services. METHODS: A mixed-methods study consisting of semistructured telephone interviews with 28 EAPs about IPV-related services and a national web-based survey of 1765 women regarding their interactions with EAPs when seeking IPV-related assistance. Data were collected in the fall of 2008. RESULTS: EAPs provide fairly extensive services to individuals experiencing IPV. Satisfaction with EAP services for IPV was significantly associated with annual income and the type of help received from the EAP, but not with type of IPV experienced. EAP representatives described challenges with accurately identifying IPV victims and women expressed concerns with confidentiality. CONCLUSIONS: Future efforts to enhance the ability of EAPs to respond effectively to IPV should address confidentially and strengthen how IPV-related assistance is delivered.

The differential risk factors of physically forced and alcohol- or other drug-enabled sexual assault among university women.

The Campus Sexual Assault Study examined whether undergraduate women's victimization experiences prior to college and lifestyle activities during college were differentially associated with the type of sexual assault they experienced: physically forced sexual assault and incapacitated sexual assault. Self-reported data collected using a Web-based survey administered to more than 5,000 undergraduate women at two large public universities indicated that victimization experiences before college were differentially associated with the risk of experiencing these two types of sexual assault during college. Women who experienced forced sexual assault before college were at very high risk of experiencing forced sexual assault during college (odds ratio [OR] = 6.6). Women who experienced incapacitated sexual assault before college were also at very high risk of experiencing incapacitated sexual assault during college (OR = 3.7). Moreover, women's substance use behaviors during college, including getting drunk and using marijuana, were strongly associated with experiencing incapacitated sexual assault but were not associated with experiencing forced sexual assault. Implications for education and prevention programs, as well as future research directions, are discussed.

College women's experiences with physically forced, alcohol- or other drug-enabled, and drug-facilitated sexual assault before and since entering college.

OBJECTIVE: Research has shown associations between college women's alcohol and/or drug consumption and the risk of sexual assault, but few studies have measured the various means by which sexual assault is achieved. PARTICIPANTS: The authors' Campus Sexual Assault Study obtained self-report data from a random sample of undergraduate women (N = 5,446). METHODS: The authors collected data on sexual assault victimization by using a cross-sectional, Web-based survey, and they conducted analyses assessing the role of substance use. The authors also compared victimizations before and during college, and across years of study. RESULTS: Findings indicate that almost 20% of undergraduate women experienced some type of completed sexual assault since entering college. Most sexual assaults occurred after women voluntarily consumed alcohol, whereas few occurred after women had been given a drug without their knowledge or consent. CONCLUSIONS: The authors discuss implications for campus sexual assault prevention programs, including the need for integrated substance use and sexual victimization prevention programming.

Assessing the long-term impact of drug court participation on recidivism with generalized estimating equations.

Drug courts are one of the most common strategies for dealing with the large proportion of criminal offenders who are drug-involved, yet methodological limitations limit the conclusions that can be drawn from many existing evaluations of their effectiveness. The current study examined the long-term impact of drug court participation compared to regular probation on the recidivism of 475 drug-involved offenders under supervision in Hillsborough County, Florida. Using a combination of self-reported data (collected through in-person interviews at baseline, i.e., the beginning of supervision) and administrative records, the study employed a repeated measures framework (examining five 6-month time periods from baseline to 30 months post-baseline) and generalized estimating equations to compare the likelihood of being arrested between drug court participants and a matched sample of comparison offenders. The results indicate that participation in drug court was associated with a significant decrease in the likelihood of being arrested in the 12-18 months post-baseline time period. Although the drug court effect was somewhat delayed (it was not significant prior to 12 months) and short-lived (it was not significant after 18 months), the fact that significant program effects were observed during a time period that coincides with the conclusion of drug court participation for graduates and a time period well beyond initial program exposure, suggests that drug court participants are more likely than comparable offenders not exposed to drug court to remain arrest free when no longer under community supervision.

The efficacy of behavioral interventions to modify dietary fat and fruit and vegetable intake: a review of the evidence.

BACKGROUND: The evidence suggesting that nutrition, particularly dietary saturated fat and fruit and vegetable intake, is related to chronic disease risk has prompted considerable research on behavioral interventions focusing on dietary change. No clear understanding has emerged, however, of the degree to which these interventions can materially influence dietary change, or the types of intervention that are most effective and for whom. Therefore, the primary objective of the current study was to evaluate the overall effectiveness of behavioral dietary interventions in promoting dietary change related to chronic disease risk reduction. A secondary goal was to explore the relative effectiveness of specific intervention features and among different population subgroups. METHODS: We conducted an evidence-based review and secondary analysis of existing literature. Our data sources included reports of randomized controlled trials and other study designs identified from multiple searches of MEDLINE, EMBASE, PsycINFO, CINAHL, AGELINE, and AGRICOLA. We included all studies on humans (including children, adolescents, and adults) published in English since 1975 that had been conducted in North America, Europe, or Australia; that had sample sizes of at least 40 subjects at follow-up; that were not based on controlled diets; and that otherwise met inclusion criteria. Through dual review, we abstracted detailed information on study characteristics, methodology, and outcomes relating to consumption of fruits, vegetables, and fats. RESULTS: From 907 unduplicated articles originally identified, we retained 104 articles reporting on 92 independent studies. The studies were similarly successful in reducing intake of total and saturated fat, and increasing fruit and vegetable intake. More than three-quarters of the studies (17 of the 22 reporting results for fruit and vegetable intake) reported significant increases in fruit and vegetable intake, with an average increase of 0.6 servings per day. Similar consistent decreases were seen in intake of saturated fat and total fat (7.3% reduction in the percentage of calories from fat). Interventions appeared to be more successful at positively changing dietary behavior among populations at risk of (or diagnosed with) disease than among general, healthy populations. Two intervention components seemed to be particularly promising in modifying dietary behavior-goal setting and small groups. CONCLUSIONS: The majority of the interventions reviewed resulted in meaningful improvements in dietary factors behaviors associated with the prevention of chronic disease, particularly among individuals at elevated disease risk. The lack of similarity across studies in outcome measures, study design, analysis strategy, and intervention technique hampered our ability to draw broad conclusions about the most effective behavioral dietary interventions, but our findings offer insight into intervention components that may hold promise for future research efforts.

Relation between mothers' child-feeding practices and children's adiposity.

BACKGROUND: The prevalence of obesity in American children is currently estimated to be 25%. Poor nutritional habits during childhood have been directly related to pediatric obesity. OBJECTIVE: Our objective was to evaluate the relation between mothers' child-feeding practices and children's adiposity in a sample of boys and girls from 2 ethnic groups. DESIGN: A total of 74 white (25 boys and 49 girls) and 46 African American (22 boys and 24 girls) children ( plus minus SD age: 11 plus minus 1.7 y) and their mothers participated in this study. The children's body composition was assessed by dual-energy X-ray absorptiometry. The mothers' child-feeding practices were assessed with the Child Feeding Questionnaire. Dietary intake data were based on three 24-h dietary recalls conducted by use of the multiple-pass technique. RESULTS: Two subscales of the Child Feeding Questionnaire, pressure to eat and concern for child's weight, explained 15% of the variance in total fat mass in both African American and white boys and girls (P < 0.001) after correction for total lean mass and energy intake (which explained 5% of the variance in total fat mass). Ethnicity, sex, and socioeconomic status did not contribute significantly to variance in total fat mass. CONCLUSIONS: Child-feeding practices are key behavioral variables that explain more of the variance in total fat mass than does energy intake in a biethnic population of boys and girls. These findings have important implications for the prevention of obesity in children because they suggest that prevention programs need to focus on the feeding behaviors of parents in addition to the macronutrient and energy intakes of children.