ABSTRACT
AIMS: To assess the percentage of cell arrested in G2M phase by colchicine in the early detection and prognosis of oral cancer, by comparison of oral squamous cell with leukoplakia and oral submucous fibrosis (OSMF) using flow cytometry analysis. MATERIALS AND METHODS: Biopsy samples 5 each of clinically diagnosed and histopathologically confirmed case of leukoplakia, OSMF, and squamous cell carcinoma (SCC) are included in the study. RESULTS: Colchicines significantly inhibited the growth of oral SCC-9 cell line by arresting G2M phase of the cell cycle (94.90%). This cell cycle result indicated that doubling of DNA is a characteristic feature of cancer. Colchicines significantly inhibited the G2M phase of the cell cycle in leukoplakia (33.51%) as compared to control (23.60%). Colchicines marginally inhibited the G2M phase of the cell cycle in OSMF (31.83%) as compared to control (28.36%). Colchicines significantly inhibited the G2M phase of the cell cycle in SCC (63.05%) as compared to control (26.40%). CONCLUSION: DNA analysis by inhibition of G2M phase of cell cycle using colchicine facilitates early detection and also helps determine the prognosis of leukoplakia, OSMF, and oral cancer.
Subject(s)
Cell Cycle Checkpoints/drug effects , Cell Cycle Checkpoints/genetics , Cell Cycle/drug effects , Cell Cycle/genetics , Colchicine/pharmacology , Leukoplakia, Oral/genetics , Leukoplakia, Oral/pathology , Mouth Neoplasms/genetics , Mouth Neoplasms/pathology , Precancerous Conditions/genetics , Precancerous Conditions/pathology , Cell Line, Tumor , Flow Cytometry , Humans , Oral Submucous Fibrosis/genetics , Oral Submucous Fibrosis/pathologyABSTRACT
Eosinophilic ulcers are rare, benign, reactive and often self-limiting lesions of the oral cavity. Although the aetiology is not clear, trauma is believed to play a role in their development. Clinically, the lesion manifests as an isolated ulcer, with raised, indurated borders and a yellow fibrinous floor; because of its long duration it often leads to the suspicion of squamous cell carcinoma. Although the ulcer is benign in nature, a biopsy is necessary to rule out malignancy. Histopathologically, the ulcer is characterised by the presence of dense inflammatory infiltrate extending into the deeper muscle layers with sheets of lymphocytes intermixed with eosinophils. This is a case report of a 65-year-old woman with an eosinophilic ulcer on the lateral border of the tongue. The ulcer healed rapidly after an incisional biopsy and topical steroid application. The final diagnosis was achieved following clinical and histopathological examination.
Subject(s)
Eosinophilia/complications , Oral Ulcer/drug therapy , Oral Ulcer/pathology , Steroids/therapeutic use , Tongue/pathology , Administration, Topical , Aged , Biopsy , Diagnosis, Differential , Female , Humans , Steroids/administration & dosage , Tongue/drug effectsABSTRACT
Osteogenesis imperfecta is a collagen related disorder characterized by increased bone fragility and low bone mass. The important oral finding in osteogenesis imperfect is the presence of dentinogenesis imperfecta. This article presents a case of osteogenesis imperfecta (type IV B) with dentinogenesis imperfecta where a 7-year-old girl had opalacent primary teeth associated with severe bone deformity, scoliosis, barrel shaped rib cage, and short stature. The clinical, radiographic ad histologic features are reviewed along with management aspects.
