ABSTRACT
Along with the development of society and the change of disease spectrum, chronic wound is gradually becoming the core of burn and plastic surgery field. Although there have been some progresses in the diagnosis and treatment technology, the management strategy of chronic wound is still in the traditional mode stage. The development of internet of things, cloud computing, big data, artificial intelligence, and other emerging technologies is changing with each passing day, and they have rapidly penetrated into the health care field. To explore the application prospect of emerging technology in the diagnosis and treatment management of chronic wound and to plan its strategy and mode in the diagnosis and treatment of chronic wound can further promote development of discipline of burns.
ABSTRACT
Designed experiment is a new experimental mode characterized by the student-centered and toacher-oriented teaching principle.To conform with the general trend of experimental teaching reform in medica university,Biochemistry Department of Third Military Medical University attemptod to adopt this teaching mode.It was indicated that designed experiment is favorable for the enhancement of comprehensive diathesis and will be an effective way to cultivate high-quality medical intellectuals.
ABSTRACT
Preparations from Echinacea are among the most widely used herbal medicines. Most uses of Echinacea are based on the reported immunological properties. In this paper, we used callus of Echinacea augustifolia for isolation and researched the factors influencing the process of protoplasts preparation, the result indicated it was easy to isolate protoplast from buff-green Callus in E. augustifolia which was looked like granule with symmetrical character. The result showed that the best enzyme solution concentration is composed of cellulase 2.0% (w/v), pectinase1.0% (w/v), hemicellulase 0.5% (w/v), 0.7 mol/L mannitol and 50.0 x 10(4) gFW(-1) protoplasts were obtained after 8 h later. The yield of protoplasts was significantly influenced by cellulase concentration and incubation time. Finally, we summarized an integrated approach including callus induction, callus culture, isolation, purification, identification and calculation of protoplasts.
