ABSTRACT
The method of contact mirror microscopy was used for assessment of the state of posterior epithelium of rabbit corneas before and after the action of laser ultraviolet radiation, lambda = 0.337 mmc. It was found that the usage of energy density of 5-20 mwt/cm2 and time exposures [correction of expositions] of 1, 5, 10, 15 min. in the terms of follow-up from 1 hour to 3 months is harmless for cells of posterior epithelium of the cornea in experiment. The method of mirror microscopy widens criteria of in vivo objective assessment of coherent radiation action on the cornea, that is important for deciding the questions about security and optimization of energetic parameters of laser therapeutic actions.
Subject(s)
Cornea/radiation effects , Lasers , Ultraviolet Rays , Animals , Cornea/cytology , Dose-Response Relationship, Radiation , Epithelial Cells , Epithelium/radiation effects , Rabbits , Time FactorsABSTRACT
A study of the state of retinal lysosomes under the action of low-energy monochromatic coherent light has shown that helium-neon laser radiation (lambda = 632.8 nm) to possesses a lysosomotropic action, changing structural functional parameters of lysosomal membranes, and the directness and intensity of the membranotropic effect on lysosomal structures of the retina depend on energy characteristics of the radiation used.
Subject(s)
Lasers , Lysosomes/radiation effects , Retina/radiation effects , Acid Phosphatase/radiation effects , Animals , Cathepsin B/radiation effects , Cathepsin D/radiation effects , Cattle , In Vitro Techniques , Lysosomes/enzymology , Male , Rabbits , Retina/enzymology , beta-Galactosidase/radiation effects , beta-Glucosidase/radiation effectsABSTRACT
Experimental investigations were carried out in monkeys and rabbits for the purpose of quantitative and qualitative assessments of contact transscleral methods of action on intraocular, structures using ruby and AIH with Nd lasers adapted with a quartz monofiber. It was found that compression of a monofiber into the sclera produces its clarification, this providing effective transmission of radiation without any loss. Essential distinctions of the character of interaction between laser radiation and eye coats in transmission of radiation by a monofiber with its compression into the sclera allowed to substantiate and recommend for a clinical usage the contact-compression transscleral method of laser microsurgery.
