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1.
Med. oral patol. oral cir. bucal (Internet) ; 26(1): e1-e7, ene. 2021. tab, ilus, graf
Article in English | IBECS | ID: ibc-200531

ABSTRACT

BACKGROUND: Oral microorganisms produce damage through the transfer to bloodstream, colonizing other tissues or direct damage in the oral cavity. Aim to study the quantitative interactions between C. albicans and the mutans streptococci and ms serotypes in the saliva of the oral cavity of patients with Down syndrome (DS). MATERIAL AND METHODS: Included 120 patients of both genders, 60 patients with Down syndrome (DS) and 60 patients as a control group (CG). Samples of saliva were taken, and bacteria and fungi were grown on TYCSB and Saboureaud agar. Microbiological, serological and quantitative analyses were performed to determine the kind of isolated of microorganisms corresponding to the ms c, e, f and k for species S. mutans and d and g for S. sobrinus and C. albicans. Electronic scanning microscopy was employed to visualize and confirm the colonies under study. Statistics analysis included t-test proofs for matched data test, Scheffé and ANOVA. RESULTS: Forming units (CFU) per mL of saliva of C. albicans a significant difference was observed among DS < CG groups. A correlation of the C. albicans quantity and the ms count was found by age intervals however, tendencies were different in SD and CG. Also, the CFU of C. albicans was different among the serotypes of ms (c, e, f, k < d, g, h, < notyped). CONCLUSIONS: These results show a significant non-random association between these two commensal microorganisms in different patient groups


No disponible


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Young Adult , Saliva/microbiology , Mouth/microbiology , Candida albicans/growth & development , Streptococcus mutans/growth & development , Down Syndrome/microbiology , Streptococcus mutans/isolation & purification , Candida albicans/isolation & purification , Microscopy, Electron, Scanning , Colony Count, Microbial , Case-Control Studies , Serogroup
2.
Rev Chilena Infectol ; 28(3): 230-7, 2011 Jun.
Article in Spanish | MEDLINE | ID: mdl-21879149

ABSTRACT

OBJECTIVE: To evaluate the colonization of group mutans streptococci according to age, measuring the amount of bacteria in saliva with a semi-quantitative method in a population attended in public and private dental centers of the Metropolitan Region, Santiago, Chile. PATIENTS AND METHODS: Saliva samples were obtained from 14,649 patients aged 5 to 40 years, in one public and 5 private dental centers. Bacteria concentration was estimated by the comparison with a standard counting-chart. The concentration of group mutans streptococci in saliva was test by a 3-way ANOVA. RESULTS: Bacterial concentration of Streptococcus mutans related with the age of patients was significant (p < 0.001). Bacterial concentration in the preschool age was 4,7 x 10(5) CFU/mL at 5 years, while 6,0 x10(5) CFU/mL at 12 years of age, with a decrease in patients over 30 years. Bacterial concentration was significantly different in the six centers of the study. CONCLUSIONS: The semi-quantitative method was useful to determine the colonization by Streptococcus mutans according to age. This could help for identifying population at high risk of dental caries and to develop oral health prevention programs in specific populations.


Subject(s)
Colony Count, Microbial/methods , Saliva/microbiology , Streptococcus mutans/isolation & purification , Adolescent , Adult , Age Factors , Child , Child, Preschool , Chile , Dental Clinics , Female , Humans , Male , Urban Population , Young Adult
3.
Rev. chil. infectol ; 28(3): 230-237, jun. 2011. ilus, tab
Article in Spanish | LILACS | ID: lil-597593

ABSTRACT

Objective: To evaluate the colonization of group mutans streptococci according to age, measuring the amount of bacteria in saliva with a semi-quantitative method in a population attended in public and private dental centers of the Metropolitan Region, Santiago, Chile. Patients and Methods: Saliva samples were obtained from 14,649 patients aged 5 to 40 years, in one public and 5 private dental centers. Bacteria concentration was estimated by the comparison with a standard counting-chart. The concentration of group mutans streptococci in saliva was test by a 3-way ANOVA. Results: Bacterial concentration of Streptococcus mutans related with the age of patients was significant (p < 0.001). Bacterial concentration in the preschool age was 4,7 x 10(5) CFU/mL at 5 years, while 6,0 x10(5) CFU/mL at 12 years of age, with a decrease in patients over 30 years. Bacterial concentration was significantly different in the six centers of the study. Conclusions: The semi-quantitative method was useful to determine the colonization by Streptococcus mutans according to age. This could help for identifying population at high risk of dental caries and to develop oral health prevention programs in specific populations.


Objetivo: Evaluar el nivel de colonización Streptococcus grupo mutans según la edad, midiendo en saliva, con un método semi-cuantitativo, la cantidad de bacterias presente en una población que acude a centros odontológicos públicos y privados de la Región Metropolitana de Chile. Pacientes y Métodos: Se obtuvieron de 14.649 muestras de saliva de pacientes incluyendo hombres y mujeres, de 5 a 40 años, en un centro de atención estatal y en cinco de atención privada. El método de recuento fue la comparación con una tabla normada de concentraciones. El análisis estadístico incluyó ANOVA. Resultados: Se encontró que el recuento de Streptococcus grupo mutans en relación a las edades de los pacientes fue significativo (p < 0,001). El recuento de bacterias en los preescolares de 5 años fue alrededor de 4.7x10(5) ufc/ mL de saliva mientras que en los niños de 12 años fue de 6.0x10(5) ufc/mL, observándose además una disminución de la carga bacteriana en los pacientes de 30 años. A edades superiores se apreció un descenso paulatino. La concentración bacteriana fue significativamente distinta en los seis centros estudiados. Conclusiones: Este método de recuento de colonias a través de la espátula permitió determinar el grado de colonización producida por Steptococcus grupo mutans según las edades, es de utilidad para identificar la población de alto riesgo de caries dentales y su aplicación ayudaría a desarrollar programas de prevención en salud oral en poblaciones especificas.


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Male , Young Adult , Colony Count, Microbial/methods , Saliva/microbiology , Streptococcus mutans/isolation & purification , Age Factors , Chile , Dental Clinics , Urban Population
4.
Med Oral Patol Oral Cir Bucal ; 13(9): E536-9, 2008 Sep 01.
Article in English | MEDLINE | ID: mdl-18758394

ABSTRACT

OBJECTIVE: to compare the concentration and serotype of Streptococcus mutans in saliva of Down syndrome (DS), mental retarded (MR) and healthy control (C) individuals of the Región Metropolitana Sur of Santiago of Chile. DESIGN OF THE STUDY: Hundred and seventy nine male and females children and adolescents, aged between 5 to 19 years, 59 DS, 60 MR and 60 C were studied. Saliva samples were cultured in TYCSB agar for quantification, biochemical and serological tests. ANOVA and Chi-square for homogeneity tests were applied. RESULTS: C, DS and MR presented Streptococcus mutans (serotypes c, e, f) and Streptococcus sobrinus (d, g, h), but only among DS and MR non-typifiable (No-tip) Streptococcus mutans were found. MR and DS showed higher bacteria concentration scores than C (P=0.001). Serotypes showed a significant heterogeneity of concentration scores: d, g, h showed the highest and No-tip the lowest one (P = 0.037). CONCLUSIONS: No-tip bacteria were absent in C and present in MR and DS; this result indicates different immune and ecological conditions among these human groups. The score of Streptococcus mutans in saliva was higher in DS and MR than in C.


Subject(s)
Down Syndrome/microbiology , Intellectual Disability/microbiology , Mouth/microbiology , Saliva/microbiology , Streptococcus mutans/isolation & purification , Adolescent , Child , Child, Preschool , Female , Humans , Male , Young Adult
5.
Med. oral patol. oral cir. bucal (Internet) ; 13(9): 536-539, sep. 2008. tab
Article in En | IBECS | ID: ibc-67503

ABSTRACT

No disponible


Objective: to compare the concentration and serotype of Streptococcus mutans in saliva of Down syndrome (DS),mental retarded (MR) and healthy control (C) individuals of the Región Metropolitana Sur of Santiago of Chile.Design of the study: Hundred and seventy nine male and females children and adolescents, aged between 5 to 19years, 59 DS, 60 MR and 60 C were studied. Saliva samples were cultured in TYCSB agar for quantification, biochemical and serological tests. ANOVA and Chi-square for homogeneity tests were applied. Results: C, DS and MR presented Streptococcus mutans (serotypes c, e, f) and Streptococcus sobrinus (d, g, h), but only among DS and MR non-typifiable (No-tip) Streptococcus mutans were found. MR and DS showed higher bacteria concentration scores than C (P=0.001). Serotypes showed a significant heterogeneity of concentration scores: d, g, h showed the highestand No-tip the lowest one (P = 0.037).Conclusions: No-tip bacteria were absent in C and present in MR and DS; this result indicates different immune and ecological conditions among these human groups. The score of Streptococcus mutans in saliva was higher in DS and MR than in C


Subject(s)
Humans , Male , Female , Child , Adolescent , Streptococcus mutans/isolation & purification , Streptococcal Infections/epidemiology , Saliva/microbiology , Dental Care for Disabled/statistics & numerical data , Disabled Children/statistics & numerical data , Streptococcus mutans/pathogenicity , Mouth/microbiology , Down Syndrome , Intellectual Disability , Case-Control Studies
6.
Rev Med Chil ; 131(4): 412-8, 2003 Apr.
Article in Spanish | MEDLINE | ID: mdl-12870236

ABSTRACT

BACKGROUND: Salivary infection due to Streptococci mutans is considered the main microbiological risk factor for the initiation of dental caries. AIM: To evaluate a semi-quantitative method to assess Streptococci mutans salivary infection and compare it with the existing quantitative method. PATIENT AND METHODS: Saliva samples were obtained from 650 preschool children aged 2 to 6 years old using a TYCSB liquid medium for counting Streptococci mutans. Results were compared with quantitative cultures. RESULTS: There was a 99.3% linear correlation coefficient between both methods. The correlation coefficients with dental caries prevalence were 97% for the semi quantitative method and 90% for the quantitative method. CONCLUSIONS: The semi-quantitative method to assess oral infection with Streptococci mutans, is accurate enough to be used in population studies and oral infection prevention programs in developing countries.


Subject(s)
Dental Caries/epidemiology , Dental Caries/microbiology , Saliva/microbiology , Streptococcus mutans/isolation & purification , Child , Child, Preschool , Chile/epidemiology , Culture Media , Dental Caries/diagnosis , False Negative Reactions , False Positive Reactions , Female , Humans , Male , Regression Analysis , Risk Factors
7.
Mag. int. coll. dent ; 7(1): 16-22, jan. 2000. graf
Article in Spanish | LILACS | ID: lil-290351

ABSTRACT

The purpose of this epidemiological study was to find correlation among dental caries and S. mutans in schoolchildren from a Protection Center. Quantitative and semiquantitative methods were employed to determine salivary concentration of mutans streptococci. The results showed that 44 percent of children had 1-3 dental caries, while 23 percent had 4-7. A direct relationship was found with the concentration of mutans streptococci in saliva. Both methods employed showed that the caries risk level, can be stabilized approximately in 5 x 105 UFCf S. mutans/mL saliva. Biochemical and serological characterisation of the isolated streptococci mutans group revealed that 95 percent was of biotype I, serotype c (c, e, f) belonged to S. mutans specie, and of 5 percent was S. sobrinus biotype IV, serotype (d, g, h). A direct correlation between decalcified areas and the quantity of salivary S. mutans was also found. Based in these results, a prediction about people in risk of develop dental caries, can be made, and so adequate prevention programs, can be established


Subject(s)
Humans , Male , Female , Adolescent , Cariogenic Agents/chemistry , Dental Caries/microbiology , Streptococcus mutans/pathogenicity , Dental Caries , Dental Caries/epidemiology , Saliva/microbiology
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