Subject(s)
Cardiovascular Diseases/prevention & control , Health Promotion , Aspirin/therapeutic use , Brazil , Evidence-Based Medicine , Female , Humans , Hypertension/prevention & control , Meta-Analysis as Topic , Quality of Life , Randomized Controlled Trials as Topic , Risk Assessment , Risk Factors , Treatment OutcomeABSTRACT
Tecostanine (1) was isolated from Tecoma stans leaves. Its sterochemistry was elucidated as well as its antihyperglycemic activity and its affinity to opioid and nicotinic receptors. The oxalate salt of 1 did not significantly affect blood glucose levels in normoglycaemic and hyperglycaemic rats. It did not appear to interact with opioid receptors (mu type) and showed only moderate affinity to the nicotinic receptor.
Subject(s)
Alkaloids/chemistry , Alkaloids/pharmacology , Bignoniaceae/chemistry , Hypoglycemic Agents/chemistry , Hypoglycemic Agents/pharmacology , Plants, Medicinal/chemistry , Terpenes/chemistry , Terpenes/pharmacology , Animals , Crystallography, X-Ray , Magnetic Resonance Spectroscopy , Male , Mexico , Models, Molecular , Molecular Conformation , Rats , Rats, Sprague-Dawley , Receptors, Nicotinic/drug effects , Receptors, Opioid/drug effectsABSTRACT
Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 micrograms/ml and 500 micrograms/ml. respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 micrograms/ml. Virucidal activity was not observed at 62.5 micrograms/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 micrograms/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Subject(s)
Antiviral Agents/pharmacology , Aphthovirus/drug effects , Herpesvirus 1, Bovine/drug effects , Herpesvirus 1, Suid/drug effects , Plant Extracts/pharmacology , Animals , Cattle , Cell Line/drug effects , Cells, Cultured , Microbial Sensitivity Tests , Plant Extracts/toxicity , SwineABSTRACT
Crude extracts of leaves and fruits of Guarea guidona were tested antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine Cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 mug/ml and 500 mug/ml, respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 mug/ml. Virucidal activity was not observed at 62.5 mug/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 mug/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Subject(s)
Animals , Cattle , Antiviral Agents/pharmacology , Aphthovirus/drug effects , Herpesvirus 1, Bovine/drug effects , Herpesvirus 1, Suid/drug effects , Plant Extracts/pharmacology , Cell Line/drug effects , Cells, Cultured , Microbial Sensitivity Tests , Plant Extracts/toxicity , SwineABSTRACT
Two biflavonoids were isolated from the EtOH extract from leaves of Ouratea spectabilis. Their structures were established to be the novel 6,6"-bigenkwanin and 7,7"-dimethoxyagathisflavone on the basis of spectroscopic data. The inhibition of bovine lens aldose reductase exerted by these biflavones has been studied.
Subject(s)
Aldehyde Reductase/antagonists & inhibitors , Flavonoids/pharmacology , Lens, Crystalline/enzymology , Plants, Medicinal , Animals , Cattle , Flavonoids/isolation & purification , Kinetics , Molecular Structure , Plant Leaves , Structure-Activity RelationshipABSTRACT
This work describes the utilization of two groups of programs in searching for characteristic signals of NMR 13C of steroidal compounds. The first group of programs used data bases with the spectral data and a methodology that enables the choice and the search of substructures. The chemical shifts and multiplicities for each specific substructure are used as rules to identify different types and subtypes of steroidal compounds. The second one was built to apply the rules formulated by the first group of programs and to foresee any skeletal based on a spectral analysis.
