How we count counts: Examining influences on detection during shoreline surveys of marine debris.

Shoreline surveys are a common approach for documenting loads of marine macrodebris (≥ 2.5 cm). When surveys are conducted repeatedly over time and space, patterns in source, abundance, geographic distribution, and composition can be detected. Yet to realize their full potential, monitoring programs that rely on surveys must grapple with high variability in debris abundance, and appropriately manage uncertainty when reporting estimates of debris quantity. A potentially important source of bias in estimating debris loads from shoreline monitoring datasets is variability in debris detection rates. With this in mind, we conducted field experiments using common strip-transect marine debris survey protocols, designed to test detection of macrodebris. We quantified how protocol, shoreline, and debris characteristics influence the detectability of marine macrodebris. Detection rates varied according to debris distance from observer (0-5 m), number of observers, debris characteristics (size, color), and shoreline substrate. Our results highlight considerations for monitoring program design. Comparisons across datasets should be approached cautiously given differences in survey protocols and sources of bias that may affect debris density estimates should be quantified and addressed. We hope these results will inform marine debris monitoring efforts that are optimized for intended data use and impact.

Walking the talk: The responsibility of the scientific community for mitigating conference-generated waste.

An estimated 19-23 million metric tons of global plastic waste reportedly entered aquatic environments in 2016 with mounting evidence that plastic marine debris causes ecological effects across all levels of biological organization in aquatic systems. Scientific conferences generate opportunities for waste through food and beverage services, giveaways, marketing and registration materials, poster and trade exhibits, attendee travel, lodging services, and local transportation. Zero waste measures instituted at the Sixth International Marine Debris Conference resulted in the avoidance of 76,300 single-use items. Zero waste is a process defined by a spectrum of actions ranging from no reduction whatsoever to generation of absolutely no waste. Achieving 100% zero waste is very difficult. Deciding where on the spectrum you wish to land and being comfortable with that target is paramount for event planning. Planning for reduced waste takes time, funding, and determination, but environmentally-themed organizations have a responsibility to lead by example.

The influx of marine debris from the Great Japan Tsunami of 2011 to North American shorelines.

Marine debris is one of the leading threats to the ocean and the Great East Japan Earthquake and tsunami on March 11, 2011 washed away an estimated 5million tons of debris in a single, tragic event. Here we used shoreline surveys, disaster debris reports and ocean drift models to investigate the temporal and spatial trends in the arrival of tsunami marine debris. The increase in debris influx to surveyed North American and Hawaiian shorelines was substantial and significant, representing a 10 time increase over the baseline in northern Washington State where a long term dataset was available. The tsunami event brought different types of debris along the coast, with high-windage items dominant in Alaska and British Columbia and large, medium-windage items in Washington State and Oregon. Recorded cumulative debris landings to North America were close to 100,000 items in the four year study period. The temporal peaks in measured shoreline debris and debris reports match the ocean drift model solutions. Mitigation and monitoring activities, such as shoreline surveys, provide crucial data and monitoring for potential impacts should be continued in the future.

The quantification of cellular viability and inflammatory response to stainless steel alloys.

The biocompatibility of metallic alloys is critical to the success of many orthopedic therapies. Corrosion resistance and the immune response of the body to wear debris products ultimately determine the performance of these devices. The establishment of quantitative tests of biocompatibility is an important issue for biomaterials development. We have developed an in vitro model to measure the pro-inflammatory cytokine production and in this study investigated the cellular responses induced by nitrogenated and 316L stainless steel alloys in both particulate and solid form. We utilized a murine macrophage cell line, RAW 264.7, to characterize and compare the mRNA profiles of TNF-alpha and IL-1beta in these cells using real time-polymerase chain reaction (RT-PCR). Fluorescence microscopy and flow cytometry were used to probe the viability of the population and to examine the apoptotic pathway. The goals of this work were to develop improved measurement methods for the quantification of cellular inflammatory responses to biomaterials and to obtain data that leads to an enhanced understanding of the ways in which the body responds to biomaterials. Using these techniques, we observed evidence for an association between the upregulation of IL-1beta and reversible apoptosis, and the upregulation of TNF-alpha and irreversible apoptosis.