ABSTRACT
The effect of ionizing radiation (gamma-irradiation of 60Co, doses from 10 Gy to 15 kGy) on human muscle tissue was studied using a biopsy material. Destructive alterations in muscle proteins were observed beginning from the dose of 1.0 kGy: appearance of new protein fractions with molecular mass 68-160 kDa and 18-36 kDa. Resistance of muscle proteins to the trypsin effect was unaltered, while the rate of pronase-induced hydrolysis was slightly increased, about 1.2-fold. Content of water and biomechanical properties of the tissue were unaltered, but the modulus of elasticity was decreased approximately 3-fold after treatment with maximal doses of the ionizing radiation used.
Subject(s)
Muscles/radiation effects , Adult , Biomechanical Phenomena , Electrophoresis, Gel, Two-Dimensional , Humans , Hydrolysis , Male , Middle Aged , Muscle Proteins/metabolism , Muscle Proteins/radiation effects , Muscles/physiology , Radiation DosageABSTRACT
More than 100 various fractions were detected in human skeletal muscle proteins analyzed by O'Farrell two-dimensional electrophoresis. Main proteins were maintained well in individual preparations of muscle tissue after their storage within 4 days at 20 degrees. The protein fractions with molecular mass of 140-150 kD as well as alteration of other muscle proteins were found on electrophoregramms within 6 days of storage.
