[The effects of heparin on intrauterine arteries of the human non-pregnant uterus: II. The analysis of the route of action]. / Wplyw heparyny na skurcz tetnic nieciezarnej macicy ludzkiej: II. Analiza drogi dzialania.

OBJECTIVE: Our purpose was to elucidate the mechanism of heparin action on smooth muscle of small intramyometrial arteries. MATERIAL AND METHOD: Material was obtained, contractility and calcium concentration were measured as previously described. Calcium influx to the vascular cells was also measured. RESULTS: Heparin did not change basal tension of the arterial strips. The strips precontracted by K(+)-depolarisation were relaxed by heparin at concentration: 200-1000 UI/mL. The maximal relaxation never exceeded 66 +/- 5.1%. Heparin (200 UI/mL) decreased the free calcium concentration in Tyrode solution to 0.8 mM. Such a lowering of the free calcium concentration diminished vascular contractile reactions. Heparin did not disturb the basal calcium influx into vascular cells but significantly decreased that which was stimulated by K+ depolarisation. 120 min treatment with heparin resulted in reducing the arterial response to 3 x 10(-8) M vasopressin recorded in Ca-free conditions. CONCLUSIONS: 1. Our results suggest that heparin, in vitro, effects the vascular smooth muscle contractions by influence on both extracellular free calcium concentration and Ca2+ influx into cytoplasm. 2. Relaxant action of heparine, on the vascular smooth muscle, is activated by additives present in commercial preparation.

[The effects of heparin on intrauterine arteries of the human non-pregnant uterus: I. The action of clinically administered heparin]. / Wplyw heparyny na skurcz tetnic nieciezarnej macicy ludzkiej: I. Dzialanie preparatu leczniczego.

OBJECTIVE: Our purpose was to study the action of commercial unfractionated heparin on small human myometrial arteries, in vitro. MATERIAL AND METHOD: Pieces of myometrial arteries, about 1 mm. in external diameter, were obtained from premenopausal women undergoing hysterectomy. Responses of arteries to K(+)-depolarisation and vassopressin were recorded under isometric condition. Free calcium concentration was determined with ion-selective electrode. RESULTS: Commercial heparin did not change basal tension of myometrial strips. Depolarised K+ strips were relaxed by heparin in concentration-dependent manner. Inhibition of NO synthase or endothelium denudation do not significantly change the reaction to heparin of strips precontracted with K(+)-depolarisation. There was a time-dependent reduction of the arterial response to vasopressin after incubation with heparin. The calcium ion chelation caused by heparin at concentrations up to 220 IU/mL did not account for the relaxation of uterine arteries. CONCLUSION: Our results suggest that clinically observed hypotensive effect of the commercial heparin is caused by its direct action on arterial smooth muscle.

[Hemostatic disturbances in women with unexplained fetal loss]. / Zaburzenia hemostazy u kobiet z utrata ciaz o niewyjasnionej etiologii.

106 women was screened for a lupus anticoagulant. Activated partial thromboplastin time, tissue thromboplastin inhibition test and euglobulin lysis time was performed. Ten of 61 women (16%) with spontaneous multiple abortions, foetal death, intrauterine growth retardation (IUGR) and gestosis had one or two pathological results of tests while no one of the 41 women which had no pregnancy complication. Significance of difference between this two groups was verified by Fischer exact test (P less than 0.05).

[Plasma fibrinolytic activity after gynecologic surgery]. / Aktywnosc fibrynologiczna osocza po operacjach ginekologicznych.

Fibrinolytic activity and fibrinolytic potential has been estimated in women submitted of gynecologic operations. Results was referred to age, extension of the operation and character of illness. Elevation of fibrinolytic activity immediately after operation and fibrinolytic potential five days later have been stated.

Induction of labor in intrauterine fetal death with 16-phenoxy-prostaglandin-E2-methylsulfonylamide (sulprostone)--effects on uterine contractility, coagulation and kallikrein-kinin systems.

Thirty-two patients with intrauterine fetal death after the 20th week of pregnancy were treated with 1-3 intramuscular injections of 500 mg of 16-phenoxy-PGE2-methylsulfonylamide (sulprostone). Intrauterine pressure records demonstrated onset of contractions within 20 min of injection and all women aborted-delivered after in mean 11 h (4-31 h). No effect on routine hematologic and plasma parameters, kallikrein-kinin and factor XII systems was observed. It is concluded that 16-phenoxy-PGE2-methylsulfonylamide is an effective and safe oxytocic agent for the induction of labor in case of intrauterine fetal death with minimal effects on the coagulation system.

[Comparison between haemostatic parameters of full - term and premature newborns (author's transl)]. / Vergleich der Hämostaseparameter bei ausgetragenen Neugeborenen und Frühgeborenen.

Twenty-five full-term newborns were compared to 20 premature newborns for several haemostatic parameters. The latter group exhibited no clinical symptoms of respiratory distress syndrome, except for two cases. The coagulative system was activated in either group. Differences were clearly recordable from the fibrinolytic system and indicated activation of fibrinolysis in full-term newborns and relative insufficiency of the same system in premature newborns. The need for assessment of both the coagulative system and fibrinolysis in any case of premature delivery is suggested by the authors and considered necessary for diagnosis, prophylaxis, and possible therapy of respiratory distress syndrome. Activation of fibrinolysis is interpreted as a mechanism of defence against concomitant intravascular coagulation, while the absence of compensation in the fibrinolytic system of the newborn is considered to be a mechanism which might lead to hyaline membrane syndrome.