Effect of ultrasound transmission gel on ultrasound-guided fine needle aspiration cytological specimens of thyroid.

OBJECTIVE: To investigate prospectively the diagnostic impact of ultrasound coupling gel on thyroid specimens obtained under ultrasound guidance. METHODS: Patients presenting for ultrasound-guided fine needle aspiration (USG-FNA) of the thyroid were invited to participate in the study. Four specimens per nodule were collected: two using chlorhexdine wash and two using sterile, colourless ultrasound gel as couplant according to routine protocol. All slides were analysed in a blinded fashion by two senior cytologists for the presence or absence of ultrasound gel-induced artefacts. The presence of gel-induced artefacts between the two groups was analyzed using Pearson's chi-square test. Kappa statistics were used to measure the inter-rater agreement between the cytologists. RESULTS: Twenty thyroid nodules comprising 80 specimen slides were collected. On slides collected with gel, cytological artefacts were detected in 60-65% of cases compared with 10-15% of cases without gel (P<0.001). The inter-rater agreement between the two observers was very good (κ=0.84). Two of the 14 patients required repeat FNA due to non-diagnostic cytology results caused by inadequate sampling and gel-induced artefacts. CONCLUSIONS: Clinical cytopathologists, radiologists and sonographers should be aware of the potential for ultrasound gel to cause significant artefacts on cytological specimens. Our findings suggest that staff involved in USG-FNA cytology should remove the gel carefully before taking the aspirate.

Clinical care and technical recommendations for 90yttrium microsphere treatment of liver cancer.

Selective internal radiation therapy (SIRT) with (90)yttrium microspheres is a relatively new clinical modality for treating non-resectable malignant liver tumours. This interventional radiology technique employs percutaneous microcatheterisation of the hepatic arterial vasculature to selectively deliver radioembolic microspheres into neoplastic tissue. SIRT results in measurable tumour responses or delayed disease progression in the majority of eligible patients with hepatocellular carcinoma or hepatic metastases arising from colorectal cancer. It has also been successfully used as palliative therapy for non-colorectal malignancies metastatic to the liver. Although most adverse events are mild and transient, SIRT also carries some risks for serious and--rarely--fatal outcomes. In particular, entry of microspheres into non-target vessels may result in radiation-induced tissue damage, such as severe gastric ulceration or radiation cholecystitis. Radiation-induced liver disease poses another significant risk. By careful case selection, considered dose calculation and meticulous angiographic technique, it is possible to minimise the incidence of such complications to less than 10% of all treatments. As the number of physicians employing SIRT expands, there is an increasing need to consolidate clinical experience and expertise to optimise patient outcomes. Authored by a panel of clinicians experienced in treating liver tumours via SIRT, this paper collates experience in vessel mapping, embolisation, dosimetry, microsphere delivery and minimisation of non-target delivery. In addition to these clinical recommendations, the authors propose institutional criteria for introducing SIRT at new centres and for incorporating the technique into multidisciplinary care plans for patients with hepatic neoplasms.

Percutaneous intracorporeal lithotripsy of biliary calculi.

The standard management for patients presenting with bile duct calculi is endoscopic retrograde cholangiopancreatography and sphincterotomy with stone retrieval. In failed cases and for intrahepatic duct stones, there are a number of non-operative alternative extraction methods. We describe two cases of percutaneous intracorporeal lithotripsy for biliary calculi and review the literature regarding the indications, complications and success rate.

Gastrointestinal haemorrhage due to erosion of the duodenal wall by a biliary stent.

Complications from improperly placed biliary stents are not uncommon. Free loose wires from the ends of an uncovered stent can irritate and damage adjacent mucosal surfaces. Effective management can be achieved via percutaneous placement of a second stent to alter the orientation of the original stent.

Reliability of X-rays and bone scans for the assessment of changes in skeletal metastases from breast cancer.

AIMS: To examine the level of agreement among observers regarding changes between serial images of bone metastases. METHODS: Thirty-five pairs of bone X-rays and 30 pairs of bone scans were selected from the files of patients with breast cancer involving the skeleton. All images in a pair were of the same site and had been taken at least 12 weeks apart. Thirteen radiologists and 14 nuclear medicine physicians examined the X-ray and bone scan pairs, respectively. Each assessed whether the changes between sequential films represented improvement, stability or worsening. Inter-observer agreement was analysed using the kappa statistic (kappa). RESULTS: There was only fair overall agreement among radiologists regarding changes between X-rays (kappa = 0.23), but there was substantial agreement among nuclear medicine physicians for bone scan assessments (kappa = 0.62). Neither the experience of the observers nor the time between images had a significant effect on agreement. For X-rays, agreement was poorer if the response category was 'improvement' and if the type of bone lesion was mixed lytic/sclerotic. CONCLUSIONS: Evaluation of serial X-rays is unreliable for determining the response of bone metastases. Scintigraphic evaluation has a higher internal validity for the determination of response, but it should not be used in isolation from other clinical data.

MR cholangiography in the evaluation of suspected intrabiliary rupture of hepatic hydatid cyst.

We report a case of intrabiliary hydatid cyst rupture that was indirectly suspected on computed tomography, diagnosed with magnetic resonance cholangiography (MRC), and confirmed with ultrasound and endoscopic retrograde cholangiopancreatography. MRC evaluation is a highly sensitive and accurate method for diagnosing intrabiliary rupture.

Non-vascular intervention with real-time CT fluoroscopy.

The emerging technology of CT fluoroscopy (CTF) represents the first opportunity for real-time CT guidance in non-vascular intervention. As with any new technology, its efficacy requires validation before widespread application can be advocated. A review of our initial experience is presented with particular attention to room, procedure and fluoroscopy time savings, complication rates and dosimetry. Computed tomography fluoroscopy is useful for pulmonary, pelvic, retroperitoneal and other deep organ lesions that are not easily accessible by other modalities. Computed tomography fluoroscopy decreases procedure time by at least a factor of 2 compared with conventional CT (C-CT) guidance, resulting in improved throughput in a busy interventional CT department. Accurate targeting of small lesions, previously considered inaccessible, can also be achieved with CTF. Exposure to the physician's hands can be reduced to a level that is acceptable to the International Commission on Radiological Protection guidelines.

Adrenal gland and renal sonography.

Ultrasound (US) is an established imaging modality for the assessment of the kidneys and adrenal glands. The advantages include its lack of ionizing radiation, speed, multiplanar real time evaluation, and cost compared with more expensive modalities. The addition of color Doppler also permits accurate evaluation of blood flow, which is especially useful in renal assessment. As with all sonography, both renal and adrenal US are highly dependent on the operator's skill and experience and require meticulous scanning techniques. In particular, adrenal sonography can be difficult due to the lack of a satisfactory acoustic window through which the incident US beam passes. This review summarizes the various morphologic appearances seen in common renal and adrenal pathologies, many of which have characteristic US appearances enabling accurate diagnosis.

Imaging of the normal and abnormal pancreaticobiliary system with single-shot MR cholangiopancreatography: a pictorial review.

Magnetic resonance cholangiopancreatography (MRCP) is a rapidly evolving non-invasive imaging modality that produces images of the pancreatic duct and biliary tree without the need for intravenous or oral contrast. The images are equivalent to those from endoscopic retrograde cholangiopancreatography (ERCP), but the non-invasive acquisition avoids the morbidity and mortality associated with diagnostic ERCP. Magnetic resonance cholangiopancreatography is indicated in patients who require only a diagnostic ERCP, who fail an ERCP or who are unable to undergo ERCP due to altered post-surgical anatomy. Other evolving indications include triaging of patients with obstructive jaundice into percutaneous or endoscopic management drainage pathways depending on the site, length and nature of the duct obstruction, thereby potentially decreasing the number of failed or unsuccessful ERCP. Pre-operative identification of anomalous biliary anatomy and choledocholithiasis prior to laparoscopic cholecystectomy promise to modify the pre-operative and operative management of the patient in order to minimize the risk of duct injury and unnecessary intra-operative dissection and cholangiography. The advantages of the technique include its non-invasiveness, the absence of contrast administration, its relative operator independence and the ability to evaluate both sides of an obstructed duct, thereby accurately evaluating stricture morphology and length. The disadvantages of MRCP compared to ERCP include its lack of an immediate therapeutic solution to duct obstruction, procedural cost, unit availability and the inability to evaluate patients with pacemakers or ferromagnetic implants.

Retroperitoneoscopic technique for treating symptomatic caliceal diverticula.

Calculous disease in a caliceal diverticulum is a rare entity. The standard treatment currently is endoscopic surgery with marsupialization or fulguration or both with dilatation of the neck of the diverticulum. We present the fifth reported case of retroperitoneoscopic management of a caliceal diverticulum in a patient with a long history of flank pain and suggest that this treatment offers a stone-free rate comparable to that of open surgery with less morbidity than is associated with endoscopic treatments.

Magnetic resonance cholangiopancreatography: non-invasive imaging for the biliary tree and pancreatic duct.

Producing images similar to those acquired by the invasive procedures of endoscopic retrograde cholangiopancreatography (ERCP) or percutaneous transhepatic cholangiography, magnetic resonance cholangiopancreatography (MRCP) is indicated in patients who are unable to undergo ERCP or have had previously unsuccessful ERCP. It is used increasingly in non-invasive evaluation of the pancreaticobiliary tree in cases where the need for intervention during ERCP is expected to be low. MRCP may help in identifying anomalous biliary anatomy or choledocholithiasis before laparoscopic cholecystectomy, and in deciding between percutaneous or endoscopic treatment for patients with obstructive jaundice to decrease the rate of failed ERCP procedures.

Paediatric tracheobronchomalacia and its assessment by tracheobronchography.

Tracheobronchomalacia (TBM) is a rare condition that results in abnormal compliance of the airways with airway collapse being most marked in expiration. In a series of 28 patients, it was observed that a majority of cases presented with malacia involving the trachea (64%) and the left main bronchus (64%) alone. The right main bronchus was involved in combination with the trachea in 32%, but rarely was involved by itself. Most patients demonstrated associated congenital abnormalities, such as tracheo-oesophageal fistulae, vascular rings or congenital heart disease (78%). A small proportion were premature infants with no obvious associated abnormality (11%). Evaluation of the dynamic process, tracheobronchomalacia; requires 'real-time' investigation. While computed tomography (CT) and magnetic resonance imaging (MRI) have their role in the management of chronic airway obstruction in the child, only cine-CT, bronchoscopy, fluoroscopy and tracheobronchography provide real-time assessment for the evaluation of a dynamic process such as TBM. In our experience, tracheobronchography provides excellent anatomic, physiologic and therapeutic information in the assessment of those infants with primary or secondary TBM. By using continuous positive airway pressure in incremental doses, the amount of positive pressure required to maintain an open airway in expiration is a valuable adjunct to the clinical management of the patient. When careful attention is paid to technique, tracheobronchography is a safe investigation in experienced hands.

Image-guided percutaneous hepatic biopsy: effect of ascites on the complication rate.

PURPOSE: To determine if image-guided percutaneous hepatic biopsy is contraindicated in patients with ascites. MATERIALS AND METHODS: The records of 476 patients (173 with ascites and 303 without) who underwent image-guided hepatic biopsy were reviewed retrospectively for number of passes, type of needle, and indications. Coagulopathy was corrected with appropriate blood products before biopsy. Complications were classified as minor (decrease in hematocrit value not necessitating treatment) of major (bleeding that necessitated transfusion or surgery or resulted in death). RESULTS: Major complications occurred in six patients with ascites and 10 without. Minor complications occurred in 10 patients with ascites and 15 without. With ascites, all major complications necessitated blood transfusions but not surgery. Five patients with major complications had a documented moderate or severe amount of perihepatic ascites. without ascites, nine of the 10 patients required blood transfusions and one required surgery. No deaths occurred in either group. CONCLUSION: Perihepatic ascites does not statistically significantly affect the major of minor complication rate of image-guided percutaneous hepatic biopsy.

Transjugular liver biopsy: a prospective study in 43 patients with the Quick-Core biopsy needle.

PURPOSE: To evaluate the efficacy and complication rate of the Quick-Core biopsy needle system compared with traditional transjugular biopsy needle systems. MATERIALS AND METHODS: Between January 1994 and April 1995, 43 patients underwent transjugular liver biopsy with the Quick-Core system; 18-, 19-, and 20-gauge needles were used in 28, 13, and two patients, respectively. Histologic diagnoses, specimen dimensions, and adequacy of the biopsy sample were determined. Immediate and delayed complications were recorded. RESULTS: A total of 118 biopsy specimens were obtained with an average of 2.7 passes per patient. Biopsy was successful in 42 of 43 patients (98%); one specimen contained renal parenchyma. Of the specimens that contained liver tissue, 100% were adequate. Mean maximum sample lengths were 1.1 and 1.5 cm with the 18- and 19-gauge needles, respectively. The procedural complication rate of 2% was due to puncture of the liver capsule in one patient, but no clinical manifestations occurred. No delayed complications occurred in any patient. CONCLUSION: The Quick-Core biopsy system produces consistently satisfactory, reproducible specimen cores with a very low complication rate.

Human bladder carcinoma cell lines as indicators of oncogenic change relevant to urothelial neoplastic progression.

Analysis of human tumour-derived cell lines has previously resulted in the identification of novel transformation-related elements and provided a useful tool for functional studies of different genes. To establish the utility of such cell lines as indicators of change relevant to urothelial cancer, we have characterised the expression of five genes (p53, MDM2, Rb, E-cadherin, APC) within a panel of human bladder carcinoma cell lines. Using single-strand conformation polymorphism (SSCP) and direct sequencing, p53 mutations were identified in 7/15 (47%) cell lines reflecting events reported in bladder tumours. Immunohistochemical analysis of p53 in cultured cells and in paraffin-embedded sections of xenografts from the cell line panel revealed discordant results. An absence of p53 nuclear staining was associated with an exon 5 mutation in EJ and with multiple p53 mutations found in J82. Two cell lines positive for p53 staining in the absence of detectable mutation displayed overexpression of MDM2 (PSI, HT1197) in Western blot analysis. Loss or aberrant Rb expression was recorded in 5/15 (TCCSUP, SCaBER, 5637, HT1376, J82) cell lines. Absence of E-cadherin was recorded in 5/15 cell lines (TCCSUP, EJ, KK47, UM-UC-3, J82) with loss of alpha-catenin in immunoprecipitated E-cadherin complexes of CUBIII. Western blot analysis of APC revealed a truncated protein in 1/15 (CUBIII) cell lines. The characterisation of oncogenic events within this panel of human bladder carcinoma cell lines establishes a representation of change observed in bladder tumours and better defines the genotypic background in these experimental human cell models of neoplastic progression.

Presurgical evaluation of the liver transplant candidate.

Determination of liver transplant candidacy requires an integrated approach contingent upon clinical, surgical, and radiologic evaluation. Imaging patients with end-stage liver disease is a challenging yet fascinating task. Chest radiographs, ultrasound with Doppler, and unenhanced and biphasic contrast CT provide sufficient preoperative information about the majority of patients. Problematic cases, however, may require a multimodality approach, for which chest CT, invasive abdominal CT techniques, MR imaging, cholangiography, angiography, and biopsy have all been useful adjuncts. In addition to providing important information for surgical planning, the radiologist must carefully look for any evidence of malignancy or other conditions that jeopardize successful OLTX and threaten meaningful postoperative survival.

Optimizing CT portography: a prospective comparison of injection into the splenic versus superior mesenteric artery.

PURPOSE: To evaluate whether computed tomographic arterial portography (CTAP) is best performed with injections in the superior mesenteric artery (SMA) or the splenic artery. MATERIALS AND METHODS: Seventy-one studies were performed with injection into the SMA (n = 37) or splenic artery (n = 34) of 150 mL of contrast material at 1.5 mL/sec and 20-second delay for both groups. Images were reviewed for location and type of nontumoral perfusion abnormalities. The degree of liver parenchymal enhancement with each technique was compared. RESULTS: Fewer nontumoral perfusion defects were seen with splenic artery (65%) versus SMA (78%) injection. Visual differences in contrast enhancement with greater attenuation in dependent portions of the liver were seen with greater frequency with SMA (41%) than with splenic artery (24%) injection. Contrast enhancement that obscured detail in the right lobe was seen only with SMA injections (16%). Greater parenchymal enhancement (up to 18 HU) at all time intervals was seen with splenic artery injection. CONCLUSION: Because of greater parenchymal enhancement and fewer nontumoral perfusion abnormalities, splenic artery catheterization is the preferred technique for CTAP.