1.
J Pediatric Infect Dis Soc
; 5(3): 329-32, 2016 Sep.
Article
in English
| MEDLINE
| ID: mdl-26407257
ABSTRACT
We analyzed in parallel 27 pediatric Clostridium difficile isolates by repetitive sequence-based polymerase chain reaction (RepPCR), pulsed-field gel electrophoresis (PFGE), and whole-genome next-generation sequencing. Next-generation sequencing distinguished 3 groups of isolates that were indistinguishable by RepPCR and 1 isolate that clustered in the same PFGE group as other isolates.
Subject(s)
Bacterial Typing Techniques , Clostridioides difficile/classification , Molecular Epidemiology , Whole Genome Sequencing , Electrophoresis, Gel, Pulsed-Field , Humans , Polymerase Chain Reaction
2.
Clin Infect Dis
; 59(3): 401-3, 2014 Aug 01.
Article
in English
| MEDLINE
| ID: mdl-24785235
ABSTRACT
Surveillance testing for Clostridium difficile among pediatric oncology patients identified stool colonization in 29% of patients without gastrointestinal symptoms and in 55% of patients with prior C. difficile infection (CDI). A high prevalence of C. difficile colonization and diarrhea complicates the diagnosis of CDI in this population.
