Genetic predisposition to essential hypertension in children: analysis of 17 single nucleotide polymorphisms.

Study of 17 single nucleotide polymorphisms has been performed to determine the factors of genetic predisposition to essential hypertension. Polymerase chain reaction (PCR) with subsequent analysis of restriction fragment length, allele specific PCR or real-time PCR was used for genotyping of 17 single nucleotide polymorphisms in 14 genes in 145 children with essential hypertension and 144 healthy persons with following complex multivariate statistical analysis. Two single nucleotide polymorphisms--MMP9 (C(-1562) --> T) and NOS3 (Glu298 --> Asp)--rs3918242 and rs1799983--were shown to represent the main independent effects with the highest predictive potential (77.1% as indicated by binary logistic regression and 74.6% testing accuracy shown by Multifactorial Dimensionality Reduction). MMP9 (C(-1562 --> T) and NOS3 (Glu298 --> Asp) potentially may be used to create predictive algorithm for determination of predisposition to arterial hypertension in children.

[Possibilities of a preparation omega-3 polyunsaturated fatty acids in the treatment of patients with ventricular arrhythmias and myocardial infarction].

The aim was to study the effectiveness of prescription medication containing 90% omega-3 polyunsaturated fatty acids for 6 months on ventricular arrhythmias in patients with myocardial infarction less than a year ago. The study involved 56 patients with ventricular extrasystoles, from 500 to 1000 per day. Divided into two groups of patients we divided into I group - 30 people - received in addition to standard drug therapy of omega-3 PUFA 1 g daily, for 6 months; II group - 26 men were given only previously assigned therapies. The average age of patients in group I - 60,7+/-8,6, II group - 61+/-9,7 years (p> 0,05). ECG monitoring was performed at 3 and 6 months. Administration of highly concentration preparation of omega-3 PUFAs for 3 months reduced number of PVCs per day, frequencies of grades 2, 3, 4A, 4B, and high grade PVCs (grades 3 - 5) as a whole. These effects persisted after 6 months of treatment.

[Prevalence of HBV and HCV markers in patients of specialized medical institutions].

240 medical histories of patients admitted to Moscow clinical narcological hospital No. 17 with "opium addiction" diagnosis in 2000 and 2003 (120 histories for each year) were analyzed. From 1997 to 2003 4220 patients registered in narcological dispensary of Cherepovets city (Vologda region) were tested for HBsAg and anti-HCV. It has been shown that in new injection opiate drug users young patients (15 - 19 y.o.) predominated (41.7%). Tendency to development of drug addiction in younger age was revealed - in 2003 compared to 2001 percent of new injection drug users aged 11 - 14 years admitted to hospital raised in 1.5 times. In 2000 and 2003 in Moscow markers of HIV and/or HBV/HCV infection were detected in 84.2 and 91.7% of injection drug users respectively. Maximal rates of HBV and HCV infection in injection drug users in Cherepovets city were detected in 2001 - 11.7 and 83.1% respectively. Leading role of injection drug users in maintenance of HBVand HCV epidemic process activity in Russia was established.

[A comparative evaluation of detection of antibodies to hepatitis C virus in different immunoassay test system and verification tests]. / Sravnitel'naia otsenka rezul'tatov opredeleniia antitel k virusu gepatita C pri ispol'zovanii razlichnykh immunofermentnykh test-sistem i podtverzhdaiushchikh testov.

Control tests and subsequent comparative analysis of their results related with the detection of anti-hepatitis C virus (anti-HCV) by different ELISA systems were made at a laboratory of the "Vector-Best" Company, Novosibirsk (A), a clinical diagnostic laboratory of Infection Clinical Hospital No. 1, No. 1 (B), and at a laboratory of chronic viral infections of Andjaparidze Institute of Viral Drugs of the Russian Academy of Medical Sciences (C). Two thousand three hundred and fifty blood sera of donors and 236 blood sera of patients with acute and chronic renal pathology were examined. The comparative research made at the above facilities by different ELISA systems produced diversified results. An analysis of 2350 blood samples from donors made in 3 ELISA systems at laboratory A showed divergence in 19 (0.8%) cases. At laboratories B and C, 5 ELISA systems (a set of 236 samples) denoted differences in 28 (11.9%) cases. Significant variances were registered in confirmation of disputable results of ELISA with immunoblot, which is apparently related with differing criteria applicable to confirming the positive results from using the confirmative tests. A possibility is discussed of using different ELISA systems in screening examination.

[Histochemical properties of the heparin-protein complex of mast cells and the state of the blood coagulating system in a primary lesion focus of non-specific ulcerative colitis]. / Gistokhimicheskie svoistva geparino-belkovogo kompleksa tuchnykh kletok i sostoianie svertyvaiushchei sistemy krovi v ochage pervichnogo porazheniia pri nespetsificheskom iazvennom kolite

Histochemical characteristics of polyanionic-cationic complex of mast cells, morphology of the microcirculatory bed of the large intestine, and a functional state of the coagulating blood system in the general blood flow and in the focus of primary inflammation were studied in patients with nonspecific ulcerative colitis. In the mast cells of the diseases intestine there was found a heparin-albuminous complex, saline resistance of the latter considerably exceed that in the norm. In the focus of primary inflammation deficit of active heparin and rise in coagulating characteristics of the blood were revealed. In the microcirculatory bed of the intestine angioniatosis, stasis, and thrombosis were observed. Impairment of complex-formation in the mast cells with appearance in their cytoplasm of the resistant heparin-albuminous complex apparently caused the local deficit of heparin and initiated the thrombogenesis in the microcirculatory bed. Thrombosis of the vessels led to hemorrhages, bleeding, disorder of the vascular wall feeding and its necrosis.