ABSTRACT
OBJECTIVE: This study aims to explore the influence of microRNA-328-3p on proliferative and apoptotic abilities of hepatocellular carcinoma (HCC) cells, and the potential regulatory mechanisms. PATIENTS AND METHODS: Quantitative Real Time Polymerase Chain Reaction (qRT-PCR) was performed to detect the expression of microRNA-328-3p in 52 tumor tissue samples and paracancerous ones of HCC patients. The potential interaction between microRNA-328-3p level and clinical indicators of HCC patients was analyzed. Subsequently, the microRNA-328-3p overexpression model was established. In addition, the influence of microRNA-328-3p on the biological functions of the HCC cells was analyzed by the cell counting kit-8 (CCK-8), colony formation assay, and flow cytometry. Finally, the potential downstream gene of microRNA-328-3p was explored by bioinformatics analysis. Recovery experiments were performed to explore the regulation mechanism. RESULTS: QRT-PCR results revealed that microRNA-328-3p level in tumor tissue specimens of HCC patients was remarkably lower than that in adjacent ones, and the difference was statistically significant. Compared with patients with high expression of microRNA-328-3p, those with low expression of miR-328-3p had more advanced pathological staging and lower overall survival. The overexpression of microRNA-328-3p decreased the proliferative capacity and increased apoptotic rate in HCC cells. Subsequently, MMP-9 expression was found to be highly expressed in HCC tissues and cells, and negatively correlated with microRNA-328-3p level. In addition, microRNA-328-3p overexpression significantly down-regulated the protein expressions of CD31, Ki-67, c-Myc, MMP-2, and MMP-9. In the cell reverse experiment, the overexpression of MMP-9 could counteract the influence of the overexpressed microRNA-328-3p on proliferation and apoptosis in HCC cells, so as to regulate the malignant progression of HCC. CONCLUSIONS: MicroRNA-328-3p could inhibit the malignant progression of HCC. Its level is remarkably associated with the pathological staging and prognosis of HCC patients. In addition, it is found that microRNA-328-3p might suppress the proliferative ability and promote apoptosis of HCC cells via modulating MMP-9.
Subject(s)
Carcinoma, Hepatocellular/metabolism , Liver Neoplasms/metabolism , Matrix Metalloproteinase 9/metabolism , MicroRNAs/metabolism , Adult , Aged , Aged, 80 and over , Carcinoma, Hepatocellular/pathology , Cell Line , Humans , Liver Neoplasms/pathology , Matrix Metalloproteinase 9/genetics , MicroRNAs/genetics , Middle AgedABSTRACT
We report a tunable, pulsed multiline intracavity optical parametric oscillator (IOPO) realized in an Nd:YVO4 laser using a two-dimensionally domain engineered MgO:LiNbO3 as simultaneously an electro-optic Bragg Q switch and a multichannel optical parametric downconverter. The MgO:LiNbO3 was periodically and aperiodically poled along the crystallographic y and x axes, respectively, to simultaneously satisfy the phase-matching conditions required by the two quasi-phase-matching devices. When Q switched by 1 kHz, 300 V pulses, three signal lines at 1518, 1526, and 1534 nm were simultaneously generated, each with a peak power of â¼1 kW from the IOPO at 8.3 W diode power at 50°C. Spectral tuning of the three-line IOPO with temperature was demonstrated.
Subject(s)
Magnesium Oxide/chemistry , Niobium/chemistry , Optical Phenomena , Oxides/chemistry , LasersABSTRACT
We describe the growth of Zn(1-x)Mn(x)Se nanowires in ultrahigh vacuum seeded by Au nanodroplets. Electron microscopy reveals the formation of single-crystal c-axis wurtzite nanowires (typically 1-3 microm long) with Mn concentrations up to x approximately 0.6, accompanied by a dense horizontal undergrowth of shorter, crooked nanowires. Magnetophotoluminescence measurements show evidence for sp-d exchange effects in a reduced symmetry environment. We find that the optical emission is surprisingly dominated by the undergrowth of crooked nanowires.
Subject(s)
Magnetics , Manganese/chemistry , Nanowires/chemistry , Selenium/chemistry , Zinc/chemistry , Gold/chemistry , Materials Testing , Metal Nanoparticles/chemistry , Nanotechnology , Optics and Photonics , Particle Size , Surface PropertiesABSTRACT
We investigated the effects of Matrine on proliferation by trypan blue exclusion and differentiation by benzidine staining positive cells in K-562 cells, assayed the telomerase activity using PCR-ELISA assay, analyzed cell cycle by fluorescence-activated cell sorter analysis of the DNA content, and also determined the gene expression level of c-myc, N-ras and p53 by northern blot and dot blot analysis. The results showed that with the addition of 0.1 mg/ml Matrine, cell growth was inhibited significantly by 4 days, benizidine-positive cells rose from 1% to 2% in control cells to 15% in treated cells on day 5; treatment of K-562 cells with 0.1 mg/ml Matrine for 5 days resulted in a marked inhibition in telomerase activity, in a manner that correlated with the extent of differentiation; after exposure to Matrine for 72 h, 64.6% cells were arrested in the G1-phase of the cell cycle, the fraction of cells in S-phase had decreased from 56.9% in control cells to 24.4% in differentiated cells, and the levels of N-ras and p53 mRNA were remarkably increased for 24 and 48 h, respectively, c-myc mRNA expression level declined for 24 h and was inhibited significantly for 48 h. Our study confirmed that Matrine plays a significant effect on the inhibition of proliferation cells and inducing differentiation in K-562 cells.
Subject(s)
Alkaloids/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Cell Differentiation/drug effects , Oncogenes/drug effects , Cell Cycle/drug effects , Cell Division/drug effects , Gene Expression/drug effects , Genes, ras/drug effects , Humans , K562 Cells , Proto-Oncogene Proteins c-myc/genetics , Proto-Oncogene Proteins c-myc/metabolism , Quinolizines , RNA, Messenger/drug effects , RNA, Messenger/metabolism , Telomerase/antagonists & inhibitors , Telomerase/metabolism , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , MatrinesABSTRACT
The essential oil of oak moss concrete was extracted by volatile oil content equipment. The chemical compositions and their relative contents were analyzed by GC and GC/MS. A Supelco-5 fused silica capillary column (30 m x 0.32 mm i.d.; 0.25 micron thickness) and a flame ionization detector (FID) were employed in GC analysis. The temperature program included temperature increase of 4 degrees C/min from 50 degrees C to 250 degrees C, and a 10 min isothermal period at 250 degrees C. Mass spectra were obtained by electron impact at 70 eV and a source temperature of 170 degrees C. Twenty-four volatile compounds of oak mass concrete were identified, which comprised more than 83% of volatile fraction. The major components were diethyl phthalate, alpha-terpineol, cedrane and linalool.
Subject(s)
Gas Chromatography-Mass Spectrometry , Oils, Volatile/chemistry , Phthalic Acids/analysis , Quercus/chemistry , Acyclic Monoterpenes , Cyclohexane Monoterpenes , Cyclohexenes , Limonene , Monoterpenes/analysis , Terpenes/analysisABSTRACT
The headspace constituents of fresh Gardenia flower were investigated by GC/MS. The headspace volatiles were sampled by solid-phase microextraction (SPME) and dynamic headspace sampling (DHS). SPME sampling was conducted with 100 microns PDMS fiber at 28 degrees C for 60 min. In DHS sampling, purified nitrogen was used as purging gas with a flow rate at 80 mL/min for 120 min. Tenax GR(20 mesh-40 mesh) was used as adsorbent and the volatiles were eluted by ether, and concentrated to 0.5 mL for GC/MS analysis. A Supelco-wax capillary column (30 m x 0.25 mm i.d. x 0.25 micron df) was employed in GC/MS analysis. Initial oven temperature was kept at 45 degrees C for 2 min, then raised to 250 degrees C at 4 degrees C/min, and kept at 250 degrees C for 10 min. According to SPME-GC/MS analysis, the main compounds in headspace of fresh Gardenia flower included farnesene(64.86%), cis-ocimene(29.33%), linalool(2.74%), cis-3-hexenyl tiglate(1.34%), methyl benzoate(0.25%). Results obtained from SPME and DHS sampling were also compared. In this study, SPME afforded a simpler and more sensitive sampling method, and much more accurate information about headspace volatiles of Gardenia flower.
Subject(s)
Flowers/chemistry , Gardenia/chemistry , Monoterpenes/analysis , Acyclic Monoterpenes , Benzoates/analysis , Chromatography, Gas , Gas Chromatography-Mass Spectrometry/methods , VolatilizationABSTRACT
This paper reports the high performance thin-layer chromatography (HPTLC) determination of beta-carotene in tobacco. The developer used was V(benzene):V(acetone) = 4:1. The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng-0.5 microgram and 20 ng per spot of beta-carotene respectively. The method has been proved to be simple, sensitive, accurate and reproducible.
Subject(s)
Chromatography, Thin Layer/methods , Nicotiana/chemistry , beta Carotene/analysisABSTRACT
We proposed a comprehensive dynamical model for the hormone secretion of the hypothalamo-pituitary-gonadal axis in man. This model is comprehensive and more reasonable than any previous model. A large number of deductions of this model agree well with experimental results.
Subject(s)
Gonadal Steroid Hormones/metabolism , Hypothalamo-Hypophyseal System/metabolism , Models, Biological , Testis/metabolism , Albumins/metabolism , Algorithms , Cell Division , Gonadotropin-Releasing Hormone/metabolism , Humans , Leydig Cells/cytology , Leydig Cells/metabolism , Luteinizing Hormone/metabolism , Male , Pulsatile Flow , Sex Hormone-Binding Globulin/metabolism , Testis/cytology , Testosterone/metabolismABSTRACT
We propose an improved mathematical model for the secretion in the hypothalamo-pituitary-gonadal axis. We think this model is more reasonable than any previous one and can interpret a large number of experimental facts.
