Toxicity of different forms of antimony to rice plant: Effects on root exudates, cell wall components, endogenous hormones and antioxidant system.

Antimony (Sb) is a toxic element for both human and plants, but the toxic responses of plants to different forms of antimony and the associated mechanisms are unknown. This study was carried out to investigate the effects of different forms of Sb [Sb(III) and Sb(V)] on the root exudates, root endogenous hormones, root cell wall components and antioxidant systems in rice plant via three hydroponic experiments. The results showed that Sb(III) displayed a higher toxicity than Sb(V) to the plant which accumulated much more Sb in its tissues under Sb(III) exposure than that under Sb(V) exposure. Under Sb(III) exposure, most of absorbed Sb was found to be Sb(III) in the shoots and roots; however when plants were exposed to Sb(V), most of absorbed Sb in this rice plant was Sb(V). Only two kinds of endogenous hormones were detected as abscisic acid (ABA) and salicylic acid (SA). The addition of Sb(III) significantly increased the content of ABA but Sb(V) did not, probably suggesting the higher toxicity of Sb(III) than Sb(V) might be due to the stimulation of ABA content. The addition of Sb(III) significantly increased the concentration of oxalic acid but decreased the concentrations of formic, acetic and maleic acids. Sb(V) also enhanced the oxalic acid concentration at 20 mg L-1 Sb(V) treatment level but reduced the concentrations of formic and acetic acids. Different forms of Sb dose-dependently increased the content of pectin, but significantly enhanced the content of lignin in cell wall. Different forms of Sb induced oxidative stress, but rice plant triggered the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) to counteract the oxidative stress.

ArsM-mediated arsenite volatilization is limited by efflux catalyzed by As efflux transporters.

Arsenic (As) methylation is regarded as an efficient strategy for As contamination remediation by As volatilization. However, most microorganisms display low As volatilization efficiency, which is possibly linked to As efflux transporters competing for cytoplasmic As(III) as a substrate. Here, we developed two types of As biosensors in Escherichia coli to compare the As efflux rate of three efflux transporters and to further investigate the correlation between As efflux rates and As volatilization. The engineered As-sensitive E. coli AW3110 expressing arsBRP, acr3RP or arsBEC displayed a higher As resistance compared to the control. The fluorescence intensity was in a linear correlation in the range of 0-2.0 µmol/L of As(III). The intracellular As(III) concentration was negatively related to As efflux activity of As efflux transporter, which was consistent with the As resistance assays. Moreover, arsM derived from R. palustris CGA009 was subsequently introduced to construct an E. coli AW3110 co-expressing arsB/acr3 and arsM, which exhibited higher As(III) resistance, lower fluorescence intensity and intracellular As concentration compared to the engineered E. coli AW3110 expressing only arsB/acr3. The As volatilization efficiency was negatively related to As efflux activity of efflux transporters, the recombinants without arsB/acr3 displayed the highest rate of As volatilization. This study provided new insights into parameters affecting As volatilization with As efflux being the main limiting factor for As methylation and subsequent volatilization in many microorganisms.

Genomic Islands Confer Heavy Metal Resistance in Mucilaginibacter kameinonensis and Mucilaginibacter rubeus Isolated from a Gold/Copper Mine.

Heavy metals (HMs) are compounds that can be hazardous and impair growth of living organisms. Bacteria have evolved the capability not only to cope with heavy metals but also to detoxify polluted environments. Three heavy metal-resistant strains of Mucilaginibacer rubeus and one of Mucilaginibacter kameinonensis were isolated from the gold/copper Zijin mining site, Longyan, Fujian, China. These strains were shown to exhibit high resistance to heavy metals with minimal inhibitory concentration reaching up to 3.5 mM Cu(II), 21 mM Zn(II), 1.2 mM Cd(II), and 10.0 mM As(III). Genomes of the four strains were sequenced by Illumina. Sequence analyses revealed the presence of a high abundance of heavy metal resistance (HMR) determinants. One of the strain, M. rubeus P2, carried genes encoding 6 putative PIB-1-ATPase, 5 putative PIB-3-ATPase, 4 putative Zn(II)/Cd(II) PIB-4 type ATPase, and 16 putative resistance-nodulation-division (RND)-type metal transporter systems. Moreover, the four genomes contained a high abundance of genes coding for putative metal binding chaperones. Analysis of the close vicinity of these HMR determinants uncovered the presence of clusters of genes potentially associated with mobile genetic elements. These loci included genes coding for tyrosine recombinases (integrases) and subunits of mating pore (type 4 secretion system), respectively allowing integration/excision and conjugative transfer of numerous genomic islands. Further in silico analyses revealed that their genetic organization and gene products resemble the Bacteroides integrative and conjugative element CTnDOT. These results highlight the pivotal role of genomic islands in the acquisition and dissemination of adaptive traits, allowing for rapid adaption of bacteria and colonization of hostile environments.