ABSTRACT
The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
Subject(s)
DNA Fingerprinting/methods , Microsatellite Repeats/genetics , Animals , Cattle , Chromosomes, Mammalian/genetics , Genetic Variation/genetics , Male , Polymerase Chain Reaction , Polymorphism, Genetic/genetics , Y Chromosome/geneticsABSTRACT
The complete sequences of mitochondrial DNA D-loop of 140 individuals in 10 Chinese goat (Capra hiruc) breeds were analyzed by DNA sequencing technology. The results showed that the length of mtDNA D-loop in Chinese goats was 1 211-1 213 bp. There were 84 different haplotypes and 171 polymorphic sites. The mean nucleotide diversity (Pi) and haplotype diversity (Hd) were 0.02063+/+0.00225 and 0.988+/-0.003, respectively, and the average number of nucleotide differences (k) was 24.896. The results showed an abundant genetic diversity in domestic goats of China. The NJ tree indicated that there were two main branches in Chinese domestic goats, thereinto, one branch was clustered with Capra aegagrus, and Capra falconeri was clustered alone, which indicated that Capra aegagrus had more contribution to Chinese domestic goat breeds.
Subject(s)
DNA, Mitochondrial/genetics , Goats/genetics , Mitochondria/genetics , Animals , Animals, Domestic/genetics , China , DNA, Mitochondrial/analysis , DNA, Mitochondrial/chemistry , Evolution, Molecular , Genetic Variation , Haplotypes , Nucleic Acid Conformation , Sequence Analysis, DNAABSTRACT
The variation of chromosomes of Gansu Black pig was caused by infusion of Duroc blood. The chromosome length ratios of No.1,6,7,13 chromosomes in the line I of Gansu Black pig were higher than those in the line II. It showed that the homogeneity in line I was higher than in line II. The number of Ag-staining nucleolus organizers (Ag-NOR) was between 1-4 in the line I and II, the average No. of Ag-NOR was different obviously between the line I and the line II. This indicated that the activity of translation in rRNA in the line I was different in the line II.
