An active Ni(OH)2/MnCO3 catalyst with efficient synergism for alkaline methanol oxidation.

A novel Ni(OH)2/MnCO3 hybrid catalyst was developed for high-performing alkaline methanol electro-oxidation, which could well overcome the shortages of inactive MnCO3 and low intrinsic Ni(OH)2 due to the good synergistic catalysis effect from the Jahn-Teller distortion effect.

Microplastics perturb colonic epithelial homeostasis associated with intestinal overproliferation, exacerbating the severity of colitis.

A great amount of the population died due to living or working in an unhealthy environment, highlighting the critical role of environmental pollutants in inducing diseases. Microplastics are widespread environmental pollutants and have been found in various tissues of human beings, yet the risk of microplastics in the occurrence of disease, especially environmentally-related colitis, is unclear. This study focused on the effects of microplastics exposure on intestinal homeostasis and the initiation of colitis. We noticed that microplastics exposure had a limited impact on mice, as verified by no difference observed in bodyweight change, IL-1ß and IL-6 levels in jejunum and liver. Nevertheless, in the colon, the IL-1ß and IL-6 levels were slightly increased and the goblet cell number was decreased. Interestingly, we observed that crypt number and depth, the levels of intestinal stem cell markers, combined with the expression of proliferating cell nuclear antigen and proto-oncogene c-Myc were all significantly increased with microplastics treatment, indicating the overproliferation of colonic mucosa. The effect of microplastics on proliferation and differentiation of crypt was further demonstrated to be regulated by the overactivation of the Notch signaling pathway in intestinal organoids. Furthermore, microplastics exposure accelerated the development of colitis with severe bodyweight loss, diarrhea and bloody stools, macroscopic and pathological damage, and inflammation levels. Worsened liver pathological damage and inflammation in mice with colitis under microplastics exposure also were found. These results suggested that microplastics disrupted the balance between colonic epithelium self-renewal and differentiation, exacerbating the colitis, and might be an environmental-related disease risk factor.

Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage.

The gastrointestinal tract is the main target of cadmium toxicity. However, whether Akkermansia muciniphila (A. muciniphila), which has been reported to be the next generation of promising probiotics, can alleviate cadmium-induced intestinal damage has not been investigated. In this study, we found that compared to the cadmium exposure group, mice gavaged with A. muciniphila showed less severe intestinal mucosal damage, with improved bodyweight, colon length, a decline in inflammation, and significantly increased glutathione and goblet cell numbers. Meanwhile, melatonin was interestingly found to be strikingly increased after A. muciniphila treatment. We then demonstrated that melatonin also could ameliorate the intestinal mucosal damage caused by cadmium through scavenging reactive oxygen species (ROS) and increasing the number of goblet cells. Furthermore, mice treated with inhibitors had a low level of melatonin and could not reproduce the beneficial effects of the A. muciniphila. Our results implied that the regulation of melatonin production by A. muciniphila is associated with an increase in enterochromaffin cells number, which determine melatonin secretion. This study indicated that the A. muciniphila-melatonin axis reduces cadmium-induced damage by increasing the goblet cells and scavenging the ROS, which may guide the prevention of the toxic effects of heavy metals.

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide.

N6-methyladenosine (m6A) is the most abundant internal modification that widely participates in various immune and inflammatory responses; however, its regulatory mechanisms in the inflammation of liver induced by lipopolysaccharide in piglets remain largely unknown. In the present study, piglets were intraperitoneally injected with 80 µg/kg LPS or an equal dose of sterile saline. Results indicated that LPS administration increased activities of serum alanine aminotransferase (ALT), induced M1 macrophage polarization and promoted secretion of inflammatory cytokines, and finally led to hepatic lesions in piglets. The NOD1/NF-κB signaling pathway was activated in the livers of the LPS group. Moreover, the total m6A level was significantly elevated after LPS treatment. MeRIP-seq showed that 1166 and 1344 transcripts contained m6A methylation in control and LPS groups, respectively. The m6A methylation sites of these transcripts mainly distributes in the 5' untranslated region (5'UTR), the coding sequence (CDS), and the 3' untranslated region (3'UTR). Interestingly, these genes were mostly enriched in the NF-κB signaling pathway, and LPS treatment significantly changed the m6A modification in NOD1, RIPK2, NFKBIA, NFKBIB, and TNFAIP3 mRNAs. In addition, knockdown of METTL3 or overexpression of FTO both changed gene levels in the NOD1/NF-κB pathway, suggesting that activation of this pathway was regulated by m6A RNA methylation. Moreover, the alteration of m6A RNA methylation profile may be associated with the increase of reactive oxygen species (ROS), HIF-1α, and MAT2A. In conclusion, LPS activated the NOD1/NF-κB pathway at post-transcriptional regulation through changing m6A RNA methylation, and then promoted the overproduction of proinflammatory cytokines, ultimately resulting in liver inflammation and damage.

Effect of Bacillus coagulans on maintaining the integrity intestinal mucosal barrier in broilers.

Bacillus coagulans (B. coagulans), a spore-forming bacteria, has been further studied for its high tolerance to extreme environmental stressors and probiotic characteristics. But the modulatory effect of B. coagulans on the intestinal mucosal barrier remains unclear. To investigate the effects of B. coagulans on intestinal mucosal barrier, 1-day-old broiler chickens were orally administrated with 108 CFU/mL B. coagulans for consecutive 42 days. In this study, the body weight, jejunum villus height and crypt depth of broiler chickens were significantly increased after B. coagulans treatment. B. coagulans also increased the contents of total protein (TP) and albumin (ALB) in serum, and reduced the contents of low-density lipoprotein (LDL-C), blood urea nitrogen (BUN) and triglyceride (TG). In addition, B. coagulans improved the intestinal flora, significantly increasing the relative abundance of beneficial bacteria in the intestine. The ability of B. coagulans to enhance innate immunity is observed by the increased number of goblet cells and the decreased mRNA expression of IL-1ß, IL-6, TNF-α, and sIgA content. Moreover, B. coagulans promoted intestinal epithelial proliferation through the Wnt/ß-catenin signaling pathway. This study demonstrated that B. coagulans could maintain the intestinal mucosal barrier by improving the intestinal flora, enhancing innate immunity and promoting intestinal epithelial proliferation.

A review of current and emerging approaches for Quaternary marine sediment dating.

Dating methodologies for Quaternary marine sediments play increasingly important roles in the reconstruction of paleoenvironments and paleoclimate in (paleo)oceanography. Previous reviews or studies have focused mainly on one or two methodologies, and their applications in one specific environment. With the continuing technological and methodological advances in different methods over the past few decades, an up-to-date comparison of the pros and cons of each dating methodology is needed to clearly understand their applications in marine geoscience research. In this review, we first briefly summarized the common methods of absolute dating and relative dating. These are (1) radioisotope dating with different half-lives using natural nuclides of 234Th, 210Pb, 230Th, and 226Ra, cosmogenic nuclides of 7Be, 14C, 10Be, 32Si, 26Al, 36Cl and 21Ne, and the artificial radionuclides of 137Cs, 239, 240Pu, 241Am and 129I that have been induced by atmospheric nuclear tests, accidents in nuclear plants, and discharges of radioactive wastes; (2) radiation exposure dating of luminescence and electron paramagnetic resonance (ESR) dating; and (3) stratigraphic dating of δ18O and paleomagnetic sequence. Applications and limitations from the marine terraces, estuaries, to hadal trenches have been summarized to each technique in the study of Quaternary marine geoscience extending from the Anthropocene through the Pleistocene. Finally, we introduced some emerging event dating methods, namely the arrivals of microplastics, mercury isotopes, and organic pollutant deposition that all appeared after the industrial resolution in our now changing ocean influenced by acidification, global warming, and anthropogenic activities. We ended by discussing future perspectives for reliable and high-resolution chronology by interdisciplinary methods including computer programming to better understand the natural geological evolution and predict the future changes in earth science.

Earliest parietal art: hominin hand and foot traces from the middle Pleistocene of Tibet.

At Quesang on the Tibetan Plateau we report a series of hand and foot impressions that appear to have been intentionally placed on the surface of a unit of soft travertine. The travertine was deposited by water from a hot spring which is now inactive and as the travertine lithified it preserved the traces. On the basis of the sizes of the hand and foot traces, we suggest that two track-makers were involved and were likely children. We interpret this event as a deliberate artistic act that created a work of parietal art. The travertine unit on which the traces were imprinted dates to between ∼169 and 226 ka BP. This would make the site the earliest currently known example of parietal art in the world and would also provide the earliest evidence discovered to date for hominins on the High Tibetan Plateau (above 4000 m a.s.l.). This remarkable discovery adds to the body of research that identifies children as some of the earliest artists within the genus Homo.

Discovering the forbidden Raman modes at the edges of layered materials.

The edges of layered materials have unique properties that substantially differ from the body regions. In this work, we perform a systematic Raman study of the edges of various layered materials (MoS2, WS2, WSe2, PtS2, and black phosphorus). The Raman spectra of the edges feature newly observed forbidden Raman modes, which are originally undetectable from the body region. By selecting the edge type and the polarization directions of the incident and scattered light, all forbidden Raman modes are distinctly detected. Optical simulations show that the edges of layered materials drastically distort the electromagnetic fields of both the incident and scattered light, so that the light interacts with the edges in a distinct way, which differs from its interactions with the body regions.

An updated age for the Xujiayao hominin from the Nihewan Basin, North China: Implications for Middle Pleistocene human evolution in East Asia.

The Xujiayao site in the Nihewan Basin (North China) is one of the most important Paleolithic sites in East Asia. Twenty Homo fossils, which were previously assigned to an archaic Homo sapiens group, have been excavated along with more than 30,000 lithic artifacts and ∼5000 mammalian fossil specimens. Dating of the Xujiayao hominin has been pursued since its excavation in the 1970s, but its age has remained controversial because of limitations of the dating techniques that have been applied to available materials. Here, we report new ages for the Xujiayao hominin based on combined electron spin resonance (ESR) dating of quartz in the sediments and high-resolution magnetostratigraphy of the fluvio-lacustrine sequence. The magnetostratigraphy suggests that the upper Matuyama and Brunhes polarity chrons are recorded at Xujiayao. The ESR dating results indicate a pooled average age of 260-370 ka for the Homo-bearing layer, which is consistent with its position within the middle Brunhes normal polarity chron indicated by magnetostratigraphy. This age estimate makes the Xujiayao hominin among the oldest mid-Pleistocene hominins with derived Neanderthal traits in East Asia. This age is consistent with the time when early Denisovans, a sister group of Neanderthals, appeared and colonized eastern Eurasia. Our updated age and the Neanderthal-like traits of the Xujiayao Homo fossils, particularly the Denisovan-like molar teeth, make it possible that the Xujiayao hominin could represent an early Denisovan.

Real-Time Observation of the Electrode-Size-Dependent Evolution Dynamics of the Conducting Filaments in a SiO2 Layer.

Conducting bridge random access memory (CBRAM) is one of the most promising candidates for future nonvolatile memories. It is important to understand the scalability and retention of CBRAM cells to realize better memory performance. Here, we directly observe the switching dynamics of Cu tip/SiO2/W cells with various active electrode sizes using in situ transmission electron microscopy. Conducting filaments (CFs) grow from the active electrode (Cu tip) to inert electrode (W) during the SET operations. The size of the Cu tip affects the electric-field distribution, the amount of the cation injection into electrolyte, and the dimension of the CF. This study provides helpful understanding on the relationship between power consumption and retention of CBRAM cells. We also construct a theoretical model to explain the electrode-size-dependent CF growth in SET operations, showing good agreement with our experimental results.

Distinctive in-Plane Cleavage Behaviors of Two-Dimensional Layered Materials.

Mechanical exfoliation from bulk layered crystal is widely used for preparing two-dimensional (2D) layered materials, which involves not only out-of-plane interlayer cleavage but also in-plane fracture. Through a statistical analysis on the exfoliated 2D flakes, we reveal the in-plane cleavage behaviors of six representative layered materials, including graphene, h-BN, 2H phase MoS2, 1T phase PtS2, FePS3, and black phosphorus. In addition to the well-known interlayer cleavage, these 2D layered materials show a distinctive tendency to fracture along certain in-plane crystallography orientations. With theoretical modeling and analysis, these distinct in-plane cleavage behaviors can be understood as a result of the competition between the release of the elastic energy and the increase of the surface energy during the fracture process. More importantly, these in-plane cleavage behaviors provide a fast and noninvasive method using optical microscopy to identify the lattice direction of mechanical exfoliated 2D layered materials.

ESR dating of the Majuangou and Banshan Paleolithic sites in the Nihewan Basin, North China.

The fluvio-lacustrine sequences in the Nihewan Basin, northern China provide an important source of Paleolithic settlements and therefore early human occupation in the eastern Old World. Here we present electron spin resonance (ESR) chronology for the Majuangou (MJG-III, MJG-II and MJG-I) and Banshan (BS) Paleolithic sites in this basin by the quartz Ti-Li center ESR dating. Results show that the ages of MJG-III and MJG-II could be estimated to be ∼1.70 Ma (millions of years ago); of MJG-I, ∼1.40 Ma; and BS, ∼1.35 Ma. This ESR chronology is consistent with the previous magnetochronology, thus providing strong support for the earliest human presence at the high northern latitudes of Northeast Asia.

Monitoring and control of UV and UV-TiO2 disinfections for municipal wastewater reclamation using artificial neural networks.

The use of ultraviolet (UV) irradiation as a physical wastewater disinfection has increased in recent years, especially for wastewater reuse. The UV-TiO(2) can generate OH radicals, which is highly effective to inactivate microorganisms in wastewater disinfection. However, both UV and UV-TiO(2) disinfections create multiple physical, chemical, and bio-chemical phenomena that affect their germicidal efficiency. It is difficult to build a precise control model using existing mathematic models. This study applies artificial neural network (ANN) models to control UV and UV-TiO(2) disinfections. Experimental results indicate that the ANN models, which precisely generate relationships among multiple monitored parameters, total coliform counts in influent and effluent, and UV doses, can be used as control models for UV and UV-TiO(2) disinfections. A novel ANN control strategy is applied to control UV and UV-TiO(2) disinfection processes to meet three total coliform count limits for three wastewater reuse purposes. The proposed controlled strategy effectively controls UV and UV-TiO(2) disinfection, resulting in acceptable total coliform counts in effluent for the three wastewater reuse purposes. The required UV doses for UV-TiO(2) disinfection were lower than those for UV disinfection, resulting in energy saving and capacity reduction of 13.2-15.7%.