ABSTRACT
Although the main stem node number of soybean [Glycine max (L.) Merr. ] is an important yield-related trait, there have been limited studies on the effect of plant density on the identification of quantitative trait loci (QTL) for main stem node number (MSNN). To address this issue, here, 144 four-way recombinant inbred lines (FW-RILs) derived from Kenfeng 14, Kenfeng 15, Heinong 48, and Kenfeng 19 were used to identify QTL for MSNN with densities of 2.2 × 105 (D1) and 3 × 105 (D2) plants/ha in five environments by linkage and association studies. As a result, the linkage and association studies identified 40 and 28 QTL in D1 and D2, respectively, indicating the difference in QTL in various densities. Among these QTL, five were common in the two densities; 36 were singly identified for response to density; 12 were repeatedly identified by both response to density and phenotype of two densities. Thirty-one were repeatedly detected across various methods, densities, and environments in the linkage and association studies. Among the 24 common QTL in the linkage and association studies, 15 explained a phenotypic variation of more than 10%. Finally, Glyma.06G094400, Glyma.06G147600, Glyma.19G160800.1, and Glyma.19G161100 were predicted to be associated with MSNN. These findings will help to elucidate the genetic basis of MSNN and improve molecular assistant selection in high-yield soybean breeding.
ABSTRACT
MicroRNAs (miRNAs) are noncoding RNAs of ~22 nucleotides in length, which serve an important role in numerous diseases. Asthma is a chronic airway inflammatory disease, which is the most common chronic disease among children. The role of miRNA (miR)16 in asthma is unclear. The objective of the present study was to examine the underlying molecular mechanism of the involvement of miR16 in asthma. A total of 72 volunteers diagnosed with asthma consented to participate in the study, of whom 52 participants were identified to be sensitive to salmeterol and 20 participants were identified to be resistant to salmeterol. Receiver operating characteristic (ROC) curve analysis was performed to compare the expression levels of serum miR16 between the sensitive and resistant groups, and to confirm the association between the expression level of serum miR16 and forced expiratory volume in 1 sec (FEV1). In silico analysis, a luciferase assay, reverse transcriptionquantitative polymerase chain reaction analysis and western blotting were performed to elucidate the molecular mechanism underlying the role of miR16 in asthma. ROC results demonstrated that the serum miR16 level may function as a biomarker to predict the response to salmeterol therapy, and the miR16 expression level displayed a significant negative correlation with FEV1. According to the in silico analysis, adrenoreceptor ß2 (ADRB2) was a direct target of miR16, and it was further confirmed by luciferase assay that 25 nM miR16 mimic had an inhibitory effect on the luciferase activity of the wildtype ADRB2 3' untranslated region (UTR); the inhibitory effect on the luciferase activity of the wildtype ADRB2 3'UTR was stronger with 50 nM miR16 mimic, and strongest with 75 nM miR16 mimic, whereas the luciferase activity of the mutant ADRB2 3'UTR in cells was similar following treatment with 0, 25, 50 or 75 nM miR16 mimic. miR16 reduced the mRNA and protein expression levels of ADRB2 in a dosedependent manner. These results identified that miR16 may be used as a predictive biomarker of therapeutic response in asthma.