Removal of Nanoplastics from Copollutant Systems Using Seaweed Cellulose Nanofibers.

Nanoplastic pollution poses a significant global concern for public health due to the potential toxicity it induces in the human body through food and water intake. Consequently, the urgent task of removing nanoplastics, especially from water resources, is paramount for enhancing food safety, and developing eco-friendly materials capable of efficiently removing nanoplastics is crucial. In this context, we propose the use of biodegradable anionic seaweed cellulose nanofibers (TEMPO-mediated seaweed cellulose nanofibers, TCNFs) and cationic seaweed cellulose nanofibers (quaternized seaweed cellulose nanofibers, QCNFs) for nanoplastic removal in both single- and copollutant systems. In our experiments under simulated practical conditions, we revealed that TCNFs and QCNFs achieved an average removal efficiency of 98.71% against nanoplastic particles. Moreover, TCNFs and QCNFs exhibited higher adsorption capacities compared to those of existing materials, potentially offering a cost-effective advantage. Toxicity assessments conducted with mammalian cells further confirmed the biosafety of TCNFs and QCNFs. This study contributes to the scientific and theoretical understanding of using edible seaweed as well as offers promising solutions for food safety control in an efficient, cost-effective, and eco-friendly manner.

Molecular insight into binding behavior of caffeine with lactoferrin: Spectroscopic, molecular docking, and simulation study.

The majority of bioactive substances in the human diet come from polyphenols. Here, we use spectroscopy, molecular docking, molecular dynamics simulations, and in vitro digestion to look at the relationship between caffeine (CAF) and bovine lactoferrin (BLF). The correlation analysis of the CAF-BLF fluorescence quenching process revealed that the reaction was spontaneous and that the CAF-BLF fluorescence quenching process may have been static. The predominant intrinsic binding forces were hydrogen bonds and van der Waals forces, which were also supported by molecular docking and molecular dynamics simulations. Through Fourier infrared and circular dichroism spectroscopy experiments, it was found that CAF changed the secondary structure of BLF and might bind to the hydrophobic amino acids of BLF. Compared with BLF, CAF-BLF showed inhibitory effects on digestion in simulated in vitro digestion. It will be helpful to better understand the interaction between CAF and BLF and provide the basis for the development of innovative dairy products.

Molecular dynamics simulation and in vitro digestion to examine the impact of theaflavin on the digestibility and structural properties of myosin.

In this study, the interaction mechanism between theaflavin and myosin was explored to confirm the potential application of theaflavin in the meat protein system. A series of theaflavin and myosin solutions were prepared for spectroscopic studies. Spectroscopy results showed that theaflavins formed complexes with myosin and affected the microenvironment of myosin. And that addition of theaflavin cause static quenching of the myosin solution. Theaflavin and bovine myosin combined through hydrophobic interaction to form a complex, and gradually increasing the temperature was conducive to the binding of theaflavin and bovine myosin. This interaction results in a decrease in the α -helix content of myosin. Molecular dynamics simulation results confirmed that hydrophobic interactions and hydrogen bonds made the protein structure more compact and stable. And the in vitro digestion process was simulated. The results showed that the addition of theaflavin could significantly reduce the digestibility of myosin.