ABSTRACT
The ent-kaurane diterpenoid weisinensis B shows significant cytotoxicity to human chronic myeloid leukemia K562 cells. It inhibits cell growth at low concentration and kills cells at high concentration. The compound induced cell apoptosis and necrosis mainly associated with G2/M phase cell cycle arrest and the ROS generation is the early event in weisiensin B induced cell apoptosis.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Diterpenes/pharmacology , Isodon/chemistry , Cell Cycle/drug effects , Cell Death/drug effects , Cell Survival/drug effects , Humans , K562 Cells , Necrosis , Reactive Oxygen Species/metabolismABSTRACT
OBJECTIVE: To study the chemical constituents from Eremosparton songoricum. METHOD: The compounds were isolated with silica gel column chromatography and the structures of these compounds were elucidated by means of spectral analysis. RESULT: The seven compounds were identified as: 5,7,4'-trihydroxyflavone (apigenin) (I), 5-hydroxy-7,4'-dimethoxyflavone (II), 5,7-dihydroxy-3',4'-dimethoxyflavone (III), 5,7-dihydroxy-4'-methoxyflavone (acacetin) (IV), 5,7,4'-trihydroxy-3'-methoxyflavone (chrysoeriol) (V), 5,6,3',4'-tetrahydroxy-7-methoxyflavone (pedalitin) (VI) and 5,4'-dihydroxy-7,3'-dimethoxyflavone-4'-O-D-glucoside (flavogadorinin) (VII). CONCLUSION: These constituents were obtained from E. songoricum for the first time.
Subject(s)
Apigenin/isolation & purification , Fabaceae/chemistry , Flavones/isolation & purification , Flavonoids/isolation & purification , Plants, Medicinal/chemistry , Apigenin/chemistry , Flavones/chemistry , Flavonoids/chemistryABSTRACT
The protective properties of seven polyphenols against hydrogen peroxide induced DNA damage in human peripheral blood lymphocytes (PBL) were studied using single cell micro-gel electrophoresis. Hydrogen peroxide causes a concentration-dependent increase in single cell DNA strand breakage in human PBL. Quercetin and 7,8-dihydroxy-4-methyl coumarin exhibited the strongest protection, significantly inhibiting 50 microM H2O2-induced DNA damage at a range of concentrations of 3.1-25 microM. Curcumin, resveratrol and vanillin protected against DNA damage induced by 50 microM H2O2 at a range of concentrations of 6.25-25 microM, but rutin and 7-hydroxy-4-methyl coumarin failed to provide any protection even at concentrations up to 50 microM. Quercetin, 7,8-dihydroxy-4-methyl coumarin, curcumin, resveratrol and vanillin are therefore effective in protection of human single cell DNA from oxidative attack.
