[Effect of UV Light Radiation on the Coagulation of Chlorella and Its Mechanism].

Considering algae were difficult to be effectively removed in conventional water treatment process, UV radiation was used to enhance the coagulation of algae in this study. The results showed that with the increase of radiation time, the removal rates of both algae and turbidity experienced a decrease after an increase, and reached their maximum values at 50 min. When the dosage of PAC was 5 mg x L(-1), the removal rates of algae and turbidity of the radiated sample were 20.1% and 18% higher than the blank sample, respectively. When pH ranged from 6 to 9, the coagulation efficiency varied little. At pH 8 and with a radiation time of 50 min, the removal rates of algae and turbidity reached 93.5% and 90.6%, respectively. Meanwhile, the Zeta potential reached the maximum, and the algae generated extracellular organic matter, which favored the subsequent coagulation. After radiated for 60 min, the algal cells was destroyed, leading to a release of intracellular organic matter into the solution. Accordingly, the Zeta potential decreased, which had a negative effect on the subsequent coagulation process.

[Effects of Hydraulic Loading Rate on the Removal of Pollutants from an Integrated Biological Settling Tank].

Based on the designing concepts of rotating biological disk and settling tank, a hybrid biological purification and settling tank was developed to achieve simultaneous removal of turbidity causing materials, organic matters, NH4+-N, and TP. Experimental results showed that under certain rotary speed and water quality conditions, the removal rates of both turbidity and organic matters and NH4+-N were relatively stable first and then started to decrease with the increase of hydraulic loading rate. The highest value was achieved at 0.036 g·(m2·d)-1 and 0.064 g·(m2·d)-1 respectively. Compared with turbidity and TOC and NH4+-N, the removal rate of TP was greatly influenced by hydraulic loading rate. The average TP removal was the worst at 0.064 g·(m2·d)-1. Appropriately decreasing or increasing the hydraulic load was helpful to the phosphorus removal process. Further study showed that nitrification and dephosphorization processes competed for the limited carbon source and DO in the water phase. Along the flow direction, the surface of the disc populations of microorganisms with good coordination and distribution along the characteristics ensured that the process still had certain decontamination effect under the condition of unfavorable hydraulic load, which indicated that the biological settling tank had a capacity in resisting organic loading rate shock and could be used in enhancing pollutant removal in treating micro-polluted water.

Behavior identification based on geotagged photo data set.

The popularity of mobile devices has produced a set of image data with geographic information, time information, and text description information, which is called geotagged photo data set. The division of this kind of data by its behavior and the location not only can identify the user's important location and daily behavior, but also helps users to sort the huge image data. This paper proposes a method to build an index based on multiple classification result, which can divide the data set multiple times and distribute labels to the data to build index according to the estimated probability of classification results in order to accomplish the identification of users' important location and daily behaviors. This paper collects 1400 discrete sets of data as experimental data to verify the method proposed in this paper. The result of the experiment shows that the index and actual tagging results have a high inosculation.

[Anatomic analysis of Wenchuan earthquake injuries with otorhinolaryngology symptoms].

OBJECTIVE: To analyze the injuries after the earthquake with otorhinolaryngology symptoms and the characteristics of with injuries and to provide some information when dealing with similar disasters in the future. METHODS: The basic information of earthquake-related injuries with otorhinolaryngology symptoms was collected in 11 large and medium-sized hospitals (including foreign medical teams) in Mianyang City and the surrounding four hard-hit counties. Their characteristics were analyzed together. RESULTS: There were 289 patients that have otorhinolaryngology symptoms in the 11 included hospitals, among them there were 217 cases have definite records that could be analyzed. The data showed that damage to ear and head and neck injuries as the main symptoms was large proportion of injuries suffer multiple fractures and soft tissue injuries such as skin abrasions . Nose injury and facial fractures were also common. Orbital fracture and eye damage could be combined occurred. Brain injury and skull fractures often occurred while trachea and throat injury was rare happened. CONCLUSIONS: Different disaster cause different damage. After the establishment of disaster-related injuries in professional spectrum more active and effective could be behaved in life rescue and the arrangement of personnel and material when dealing with similar situations.

Cloning of Human IgG Fc cDNA and Expression of Whole Human Anti-HBsAg Antibody in CHO Cells.

Messenger RNA was extracted from human peripheral lymphocytes and first strand cDNA was prepared by reverse-transciption. The cDNA of Fc fragment of human IgG1 was then obtained by PCR and was cloned into the pGEM T-vector. The DNA sequences encoding signal peptides of both light and heavy chains were synthesized and cloned respectively. For construction of the light chain expression plasmid, the light chain signal sequence was linked with the light chain variable and constant regions (VL-CL) which had been cloned previously by screening of phage display libraries with HBsAg. The resulting full-lenth light chain sequence was then inserted into pcDNA3.1, a mammalian expression vector. For construction of the heavy chain expression plasmid, the heavy chain signal sequence, the variable region, the first constant region (VH-CH1, cloned previously by screening of phage display libraries with HBsAg) and Fc fragment sequence were ligated to form a full-length heavy chain ORF, which was then cloned into another mammalian expression vector, pCI-DHFR1. CHO(dhfr(-)) cells were cotransfected with the above light and heavy chain expression plasmids, and cell clones expressing human anti-HBsAg antibodies were selected by G418 and methotrexate (MTX). The recombinant human antibodies were purified with protein L affinity chromatography from the cell culture medium. As human serum IgG, the recombinant IgG exhibited only one band with a molecular weight of more than 100 kD in non-reducing SDS-PAGE in reducing SDS-PAGE, however, it turned out to be two bands of approximately 50 kD and 25 kD respectively. Western-blot analysis demonstrated that the whole IgG in the non-reducing SDS-PAGE, and the heavy chain in the reducing SDS-PAGE both reacted with goat anti-human Fc antiserum.