Hyaluronic acid modulates gene expression of connective tissue growth factor (CTGF), transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth factor (VEGF) in human fibroblast-like synovial cells from advanced-stage osteoarthritis in vitro.

Intraarticular injection of hyaluronan (hyaluronic acid; HA) is the common way to treat osteoarthritis (OA) of knees. This treatment cannot only maintain the viscoelastic properties of knee but also release the OA pain. However, the exact molecular mechanism is unknown. In this study, after human synovial cells were stimulated with HA and Hylan (Synvisc) for 24 h, real-time polymerase chain reaction (real-time PCR) was used to detect the alteration of connective tissue growth factor (CTGF), transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth factor (VEGF) gene expression, which were specific genes related to pathogenesis of OA knees. Our results illustrated that both HA and Hylan might not cause cytotoxicity or apoptosis of synovial cells in serum deprivation environment. The gene expressions of TGF-beta1 and VEGF were significantly increased at the concentration of 0.1 mg/mL HA and 0.1 mg/mL Hylan, respectively (alpha < 0.05). The synovial cells with treatment of 0.1 mg/mL Hylan decreased the CTGF gene expression (0.66-fold) and VEGF (0.78-fold) compared to 0.1 mg/mL HA (alpha < 0.05). We suggested that the profile of CTGF, TGF-beta1, and VEGF gene expressions in our study might provide the rational mechanism for the therapeutic effect of hyaluronan on OA knees.

Surgery simulation analysis of anterior advancement of the tibial tuberosity.

The objective of this study is to evaluate the surgical outcome of anterior displacement of the tibial tuberosity (Maquet procedure) for reducing patellofemoral joint contact force. Thein-vivo experimental knee joint geometric data with a biomechanical model was used to do the simulation of the Maquet procedure. Six healthy young adults performed weight-bearing knee flexion-extension by ascending a one-step stair. Dynamic X-ray images of the knee were continuously recorded by a video-fluoroscopic system. These X-ray images were analysed on a computerized digitizing system to get the knee joint geometric data. Based on the continuous in-vivo geometric data, computer surgery simulation was studied on six right knees with advancement of 3, 5, 10, 15, and 20 degrees of the patellar tendon insertion. Evaluation of the simulation consequences from a biomechanical view point showed that the Maquet procedure reduced the patellofemoral joint reaction force only up to 20% at 90 degrees of knee flexion angle. The patellofemoral joint reaction force had 50% reduction only when the knee flexion angle less than 20 degrees, and only when the patellar tendon was moved out by 15 or 20 degrees. This represented nearly 1 in. of the anterior displacement of the tibial tuberosity. The results also showed that the Maquet procedure would decrease up to 20% of the force transmission efficiency of the patellofemoral mechanism, which would cause the mechanical consequences of the operation to be only minor at larger knee flexion angles more than 20 degrees. These findings suggest that the Maquet procedure is only favourable to less active or older patients having small knee flexion angle activities. RELEVANCE: Surgical procedure of anterior displacement of the tibial tuberosity has been used to reduce the patellofemoral joint contact force for treatment of symptomatic osteoarthrosis of the patellofemoral joint. In this study the patellofemoral joint reaction force had 50% reduction only when the knee flexion angle was less than 20 degrees, and only when patellar tendon was moved out nearly 1 in. Based on this result, the Maquet procedure is suggested only favourable to less active or older patients having small knee flexion angle activities.