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FEBS Lett ; 591(23): 3872-3880, 2017 12.
Article in English | MEDLINE | ID: mdl-29110302

ABSTRACT

The sucrose synthase (SUS) interactome of developing castor oilseeds (COS; Ricinus communis) was assessed using coimmunoprecipitation (co-IP) with anti-(COS RcSUS1)-IgG followed by proteomic analysis. A 41-kDa polypeptide (p41) that coimmunoprecipitated with RcSUS1 from COS extracts was identified as reversibly glycosylated polypeptide-1 (RcRGP1) by LC-MS/MS and anti-RcRGP1 immunoblotting. Reciprocal Far-western immunodot blotting corroborated the specific interaction between RcSUS1 and RcRGP1. Co-IP using anti-(COS RcSUS1)-IgG and clarified extracts from other developing seeds as well as cluster (proteoid) roots of white lupin and Harsh Hakea consistently recovered 90 kDa SUS polypeptides along with p41/RGP as a SUS interactor. The results suggest that SUS interacts with RGP in diverse sink tissues to channel UDP-glucose derived from imported sucrose into hemicellulose and/or glycoprotein/glycolipid biosynthesis.


Subject(s)
Glucosyltransferases/isolation & purification , Plant Proteins/isolation & purification , Ricinus communis/chemistry , Ricinus communis/enzymology , Ricinus/chemistry , Ricinus/enzymology , Blotting, Far-Western , Ricinus communis/genetics , Glucosyltransferases/chemistry , Glucosyltransferases/genetics , Glycoproteins/chemistry , Glycoproteins/genetics , Glycoproteins/isolation & purification , Glycosylation , Immunoprecipitation , Phylogeny , Plant Proteins/chemistry , Plant Proteins/genetics , Protein Interaction Mapping , Proteomics , Ricinus/genetics , Tandem Mass Spectrometry
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