Preparation and biological properties of ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin biomimetic composite scaffolds for bone tissue engineering.

To imitate the composition of natural bone and further improve the biological property of the materials, ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin (ZnO/HAP/CS-PEO@GEL) composite scaffolds were developed. The core-shell structured chitosan-polyethylene oxide@gelatin (CS-PEO@GEL) nanofibers which could form the intramolecular hydrogen bond and achieve an Arg-Gly-Asp (RGD) polymer were first prepared by coaxial electrospinning to mimic the extracellular matrix. To further enhance biological activity, hydroxyapatite (HAP) was grown on the surface of the CS-PEO@GEL nanofibers using chemical deposition and ZnO particles were then evenly distributed on the surface of the above composite materials using RF magnetron sputtering. The SEM results showed that chemical deposition and magnetron sputtering did not destroy the three-dimensional architecture of materials, which was beneficial to cell growth. The cell compatibility and proliferation of MG-63 cells on ZnO/HAP/CS-PEO@GEL composite scaffolds were superior to those on CS-PEO@GEL and HAP/CS-PEO@GEL composite scaffolds. An appropriate amount of ZnO sputtering could promote the adhesion of cells on the composite nanofibers. The structure of bone tissue could be better simulated both in composition and in the microenvironment, which provided a suitable environment for cell growth and promoted the proliferation of MG-63 cells. The biomimetic ZnO/HAP/CS-PEO@GEL composite scaffolds were promising materials for bone tissue engineering.

Biomimetic composite scaffold of hydroxyapatite/gelatin-chitosan core-shell nanofibers for bone tissue engineering.

Good biocompatibility and osteogenesis of three-dimensional porous scaffolds are critical for bone tissue engineering. In this work, biomimetic hydroxyapatite/gelatin-chitosan core-shell nanofibers composite scaffolds have been fabricated to mimic both the specific structure and the chemical composition of natural bone. The coaxial electrospinning technique was introduced to prepare gelatin-chitosan core-shell structured nanofibers mat which formed three-dimensional porous structure for promoting cells growth. The gelatin-chitosan core-shell nanofibers formed Arginine-Glycine-Aspartic acid (RGD)-like structure to mimic the organic component of natural bone extracellular matrix. Hydroxyapatite (Ca10(PO4)6(OH)2, HAP), as the major mineral constituent of native bone, was then deposited onto the surface of gelatin-chitosan core-shell structured nanofibers by a wet chemical method. Compared with chitosan nanofibers, gelatin nanofibers and chitosan-gelatin composite nanofibers, gelatin-chitosan core-shell structured nanofibers improved the mineralization efficiency of hydroxyapatite and formed a homogeneous HAP deposit. When Human osteoblast like cell line (MG-63) were cultured on the materials, the results showed that hydroxyapatite deposited on the gelatin-chitosan core-shell structured nanofibers could further enhance osteoblast cell proliferation. The biomimetic composite scaffolds could be suggested as a promising material to promote osteoblast cell growth in bone tissue engineering.