In-fiber Mach-Zehnder interferometer based on hollow optic fiber for metal ion detection.

A new, to the best of our knowledge, in-fiber Mach-Zehnder interferometric sensor is proposed and experimentally demonstrated for detecting Cu2+ ions in an aqueous environment. The sensor is fabricated simply and cost-effectively by arc-fusing a short section of hollow optical fiber between two standard single-mode fibers and is functionalized by depositing chitosan and poly(acrylic acid) bilayers using electrostatic self-assembly. The proposed sensor shows a linear response with sensitivity of 42 nm/mM in the Cu2+ ion concentration from 0 to 40 µM. Moreover, the fiber sensor exhibits good reusability and repeatability and is a promising candidate for contamination detection in drinking water and industrial waste water.

Adapalene Inhibits Prostate Cancer Cell Proliferation In Vitro and In Vivo by Inducing DNA Damage, S-phase Cell Cycle Arrest, and Apoptosis.

Aims: Prostate cancer is a well-known aggressive malignant tumor in men with a high metastasis rate and poor prognosis. Adapalene (ADA) is a third-generation synthetic retinoid with anticancer properties. We investigated the anti-tumor activity and molecular mechanisms of ADA in the RM-1 prostate cancer cell line in vivo and in vitro. Methods: The effects of ADA on cell proliferation were estimated using the CCK-8 and colony formation assays. The wound-healing assay and the Transwell assay were employed to examine the migratory capacity and invasiveness of the cells. Flow cytometry was utilized to evaluate the cell cycle and apoptosis, and Western blotting analysis was used to assess the expression of the associated proteins. Micro-CT, histomorphological, and immunohistochemical staining were used to assess the effects of ADA on bone tissue structure and tumor growth in a mouse model of prostate cancer bone metastasis. Result: ADA dramatically inhibited cell proliferation, migration, invasiveness, and induced S-phase arrest and apoptosis. ADA also regulated the expression of S-phase associated proteins and elevated the levels of DNA damage markers, p53, and p21 after ADA treatment, suggesting that the anti-tumor effect of ADA manifests through the DNA damage/p53 pathway. Furthermore, we observed that ADA could effectively inhibited tumor growth and bone destruction in mice. Conclusion: ADA inhibited prostate cancer cell proliferation, elicited apoptosis, and arrested the cell cycle in the S-phase. ADA also slowed the rate of tumor growth and bone destruction in vitro. Overall, our results suggest that ADA may be a potential treatment against prostate cancer.

Alcohol Selective Optical Sensor Based on Porous Cholesteric Liquid Crystal Polymer Networks.

A responsive hydrogen-bonded cholesteric liquid crystal polymer (CLCP) film with controlled porosity was fabricated as an optical sensor to distinguish between methanol and ethanol in alcohol solutions. To facilitate responding the alcohols, porosity was generated by removing the nonreactive liquid crystal agent, and the hydrogen bridges of CLCP were broken. The sensitivities of CLCPs to ethanol and methanol were obtained by monitoring the wavelength shifts of the transmission spectrum at different alcohol concentrations and ratios of methanol/ethanol. Changes in the central wavelength of the CLCP network transmission spectrum allowed the methanol-ethanol ratio to be discriminated. A linear relationship between wavelength shift of CLCP networks and alcohol concentration was obtained experimentally, and the sensor characteristics were explored. The sensitivities of the CLCPs were 1.35 and 0.18 nm/% to ethanol and methanol, respectively. The sensing sensitivity of cholesteric networks to alcohol molecules increased as the methanol-ethanol ratio declined. Therefore, CLCP could act as a stimuli-responsive material to distinguish the concentrations of acetone and ethanol in mixed solutions. Furthermore, the impact of UV intensity for curing a CLC mixture on the sensing sensitivity to the different alcohol concentrations was also studied. The higher UV intensity could enhance the sensitivity to alcohol molecules and distinguishing ability between methanol and ethanol.

Effect of dual-acupoint and single-acupoint electric stimulation on postoperative outcomes in elderly patients subjected to gastrointestinal surgery: study protocol for a randomized controlled trial.

BACKGROUND: Transcutaneous electric acupoint stimulation (TEAS) has shown benefits when used peri-operatively. However, the role of numbers of areas with acupoint stimulation is still unclear. Therefore, we report the protocol of a randomized controlled trial of using TEAS in elderly patients subjected to gastrointestinal surgery, and comparing dual-acupoint and single-acupoint stimulation. METHODS/DESIGN: A multicenter, randomized, controlled, three-arm design, large-scale trial is currently undergoing in four hospitals in China. Three hundred and forty-five participants are randomly assigned to three groups in a 1:1:1 ratio, receiving dual-acupoint TEAS, single-acupoint TEAS, and no stimulation, respectively. The primary outcome is incidence of pulmonary complications at 30 days after surgery. The secondary outcomes include the incidence of pulmonary complications at 3 days after surgery; the all-cause mortality within 30 days and 1 year after surgery; admission to the intensive care unit (ICU) and length of ICU stay within 30 days after surgery; the length of postoperative hospital stay; and medical costs during hospitalization after surgery. DISCUSSION: The result of this trial (which will be available in September 2019) will confirm whether TEAS before and during anesthesia could alleviate the postoperative pulmonary complications after gastrointestinal surgery in elderly patients, and whether dual-acupoint stimulation is more effective than single-acupoint stimulation. TRIALS REGISTRATIONS: ClinicalTrials.gov, ID: NCT03230045 . Registered on 10 July 2017.

A spirituality teaching program for depression: a randomized controlled trial.

OBJECTIVE: This randomized controlled trial assessed the efficacy of a Spirituality Teaching Program to treat unipolar major depression. METHOD: A randomized controlled, assessor blinded trial design was used. A total of 84 individuals aged 18 years or older with unipolar major depression of mild to moderate severity were recruited in Calgary, Canada and randomized to two study arms: 1) Spirituality Teaching Program Group (8 week, home-based Spirituality Teaching Program); and 2) Waitlist Control Group (no intervention followed by Spirituality Teaching Program starting at week 9). Outcome measures (depression severity, response rate, remission rate) were assessed at baseline, 8, 16, and 24 weeks using the Hamilton Depression Rating Scale (HAM-D). RESULTS: The two trial groups were similar in their demographic and disease characteristics at baseline. At the 8-week point, the change in depression severity was significantly different between the two groups (change in HAM-D score: 8.5 for the Spirituality Group and 2.3 for the Waitlist Control Group, p < 0.001). The Spirituality Teaching Program Group had significantly higher response (36% vs. 4.4%, p < 0.001) and remission rates (31% vs. 4.4%, p < 0.001) than the Waitlist Control Group. The benefits remained throughout the observation period for the Spirituality Teaching Program Group participants with response rates of 56.4% at 16 weeks and 58.9% at 24 weeks. CONCLUSION: The Spirituality Program significantly reduced depression severity and increased response and remission rates. This non-drug treatment program should be investigated further as a treatment option for depression.