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1.
Article in Chinese | MEDLINE | ID: mdl-37667164

ABSTRACT

Severe acute dichlorvos poisoning is characterized by rapid onset, swift disease progression and serious complications. It frequently involves multiple organ failure (central, respiratory and circulatory systems), severe acidosis, and rare occurrences of gastric perforation. When secondary gastric perforation occurs, treatment becomes difficult and the prognosis of patients is poor. Thus, early and sufficient gastrointestinal decontamination is crucial. This article presented two cases of gastric perforation secondary to dichlorvos poisoning and discussed the causes of gastric perforation, as well is clinical diagnostic and treatment methods.


Subject(s)
Dichlorvos , Multiple Organ Failure , Humans
2.
Eur Rev Med Pharmacol Sci ; 27(1): 122-129, 2023 01.
Article in English | MEDLINE | ID: mdl-36647859

ABSTRACT

OBJECTIVE: The aim of this study is to evaluate the effects of positive intervention on the anxiety and the physiological and psychological aspects among preoperative and post-surgical patients with spinal anesthesia. PATIENTS AND METHODS: A randomized trial was conducted with an intervention group (n=58) and a control group (n=59). In the intervention group, the patients were well-informed of the details during spinal anesthesia. Multiple methods were performed to control anxiety before surgery, and nurses were not allowed to discuss the condition during surgery. Anesthesiologists were invited to visit patients to avoid excessive anxiety. RESULTS: The intervention group showed lower scores of State-Trait Anxiety Inventory (STAI) (p<0.05) than the control group 24 hours post-operation. Physiological indices such as systolic blood pressure, low frequency (LF) power, high frequency (HF) power and ration of LF/HF showed better surgery recovery (p<0.05) than the control group. The length of post-anesthesia care unit stay was also significantly shortened in the intervention group (p=0.001) compared with the control group. Positive intervention may alleviate the anxiety in surgical patients receiving spinal anesthesia and improve the physiological and psychological outcomes clinically. CONCLUSIONS: Our results provide evidence indicating that proper intervention can be promoted clinically to improve the satisfaction and quality of life of patients undergoing spinal anesthesia.


Subject(s)
Anesthesia, Spinal , Humans , Psychosocial Intervention , Quality of Life , Anxiety/prevention & control , Blood Pressure/physiology
3.
Eur Rev Med Pharmacol Sci ; 24(23): 12251-12257, 2020 12.
Article in English | MEDLINE | ID: mdl-33336744

ABSTRACT

OBJECTIVE: CircRNAs are vital factors involved in the pathological processes. This study aims to elucidate the biological functions of hsa_circ_0000337 in affecting the malignant progress of glioma. PATIENTS AND METHODS: Relative levels of hsa_circ_0000337 in 45 cases of glioma and 24 cases of normal tissues were tested. The correlation between hsa_circ_0000337 and clinical features of glioma was assessed. Proliferative and metastatic abilities of U87 and U251 cells regulated by hsa_circ_0000337 were examined by 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assay, respectively. Potential molecular mechanism of hsa_circ_0000337 on regulating glioma cell functions was clarified by bioinformatic analysis, which was further verified through rescue experiments. RESULTS: Hsa_circ_0000337 was highly expressed in glioma cases. Its level was correlated to poor prognosis of glioma. In vitro experiments obtained the conclusion that hsa_circ_0000337 accelerated proliferative and metastatic abilities of glioma cells. Serving as a ceRNA, hsa_circ_0000337 sponged miRNA-942-5p to upregulate MAT2A, thus inducing the malignant phenotypes of glioma. CONCLUSIONS: Hsa_circ_0000337/miRNA-942-5p / MAT2A axis is responsible for the deterioration of glioma. Hsa_circ_0000337 may be a potential therapeutic target for glioma.


Subject(s)
Central Nervous System Neoplasms/metabolism , Glioma/metabolism , Methionine Adenosyltransferase/metabolism , MicroRNAs/metabolism , RNA, Circular/metabolism , Up-Regulation , Binding Sites , Cell Movement , Cell Proliferation , Cells, Cultured , Central Nervous System Neoplasms/pathology , Glioma/pathology , Humans , Methionine Adenosyltransferase/genetics , MicroRNAs/genetics , RNA, Circular/genetics
4.
Eur Rev Med Pharmacol Sci ; 24(9): 5071-5081, 2020 05.
Article in English | MEDLINE | ID: mdl-32432771

ABSTRACT

OBJECTIVE: Acute lung injury (ALI) is the most common complication of sepsis, with rapid onset and high mortality. There is currently no effective treatment for ALI. Therefore, we looked for a good method of treating ALI by studying the effect and mechanism of Nesfatin-1 on ALI. MATERIALS AND METHODS: We used LPS to induce mouse and human alveolar epithelial cell line BEAS-2B to construct an ALI model. Recombinant Nesfatin-1 was administered subcutaneously to mice or used to stimulate BEAS-2B cells. We collected mouse bronchoalveolar lavage fluid and mouse lung tissue to detect changes in inflammatory factors and oxidative stress levels. In addition, we examined the expression changes of HMGB1 to study the mechanism of Nesfatin-1. RESULTS: Exogenous Nesfatin-1 significantly attenuated LPS-induced ALI and reduced inflammation levels and oxidative stress levels in mouse lung tissue. In cell experiments, Nesfatin-1 also reduced inflammation levels and oxidative stress levels in BEAS-2B cells. In addition, Nesfatin-1 reduced the expression of HMGB1 in mouse lung tissues and BEAS-2B cells, and decreased the activity of p38MAPK and NF-κB signaling pathways in the inflammation-related pathway downstream of HMGB1. However, after overexpression of HMGB1, the therapeutic effect of Nesfatin-1 on ALI was attenuated. CONCLUSIONS: Nesfatin-1 regulates the expression of HMGB1 in alveolar epithelial cells. By reducing the expression of HMGB1, Nesfatin-1 can reduce the inflammation-related signaling pathway downstream of HMGB1 to reduce the level of inflammation and oxidative stress in alveolar epithelial cells, thereby alleviating ALI.


Subject(s)
Acute Lung Injury/metabolism , HMGB1 Protein/metabolism , Inflammation/metabolism , Nucleobindins/metabolism , Oxidative Stress , Acute Lung Injury/chemically induced , Acute Lung Injury/pathology , Animals , Cells, Cultured , HMGB1 Protein/genetics , Humans , Inflammation/chemically induced , Inflammation/pathology , Lipopolysaccharides , Male , Mice , Mice, Inbred C57BL , Nucleobindins/genetics , Signal Transduction
5.
Neurogastroenterol Motil ; 22(8): 927-34, e238-9, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20426800

ABSTRACT

BACKGROUND: Chronic cholangitis caused by hepatolithiasis is a common disease in Southeast Asia. Few studies have addressed the effects of chronic cholangitis on cyclic activity of the sphincter of Oddi (SO). In this study, we investigated the changes of myoelectric activity in rabbits with chronic cholangitis in vivo and in vitro. METHODS: Chronic cholangitis was induced in rabbits by initially introducing three pieces of 2-0 silk suture and sequentially injecting E. coli into the choledochus through the tube in ductus cysticus. In in vivo experiments, myoelectric activity of SO was recorded by a circular electrode through the jejunum stump in conscious animals. In in vitro experiments, the SO was completely isolated and the myoelectric activity was recorded by a circular electrode in a 10-mL organ bath filled with Krebs solution, with or without addition of cholecystokinin-8 (CCK-8), KCl, ionomycin or induction of capacitative calcium entry (CCE). KEY RESULTS: In comparison with control and non-infected rabbits, the rabbits with chronic cholangitis showed higher levels of alkaline phosphatase and gamma-glutamyltransferase and significant pathological changes including increased inflammatory infiltration and collagen deposition in mucosae or muscular layer. Cyclic myoelectric activity of SO at phases 2 and 3 of migrating motor complex and the excitatory response to CCK-8 were dramatically decreased in animals with chronic cholangitis. Myoelectric activity of SO was also significantly decreased in vitro with or without agonists or with induction of CCE. CONCLUSIONS & INFERENCES: Myoelectric activity of SO and its response to agonists are decreased in rabbits with chronic cholangitis both in vivo and in vitro.


Subject(s)
Cholangitis/physiopathology , Electromyography/methods , Sphincter of Oddi/physiology , Sphincter of Oddi/physiopathology , Animals , Calcium/metabolism , Cholangitis/pathology , Cholecystectomy , Cholecystokinin/pharmacology , Chronic Disease , Muscle Contraction/drug effects , Muscle Contraction/physiology , Muscle, Smooth/drug effects , Muscle, Smooth/physiology , Peptide Fragments/pharmacology , Rabbits , Random Allocation , Sphincter of Oddi/drug effects , Sphincter of Oddi/pathology
6.
Int J Biol Markers ; 23(2): 83-8, 2008.
Article in English | MEDLINE | ID: mdl-18629780

ABSTRACT

AIM: To study the expression of Mina53 and its relationships with clinicopathological characteristics, antioncogene inactivation and tumor proliferation in human gastric carcinoma, and to explore the role of Mina53 in carcinogenesis and tumor progression. METHODS: Expression of Mina53 and proliferating cell nuclear antigen (PCNA) was determined in gastric carcinoma (n=79), gastric dysplasia (n=21) and normal gastric tissues (n=20), while p53 was measured in gastric carcinoma tissues by immunohistochemistry. RESULTS: Mina53 was negatively expressed in all normal mucosa tissues. Dysplasia specimens showed weakly positive staining for Mina53 in 3 of 21 cases. Elevated expression of Mina53 was observed in 72 (91.1%) of the gastric carcinomas. No significant associations were found between Mina53 and clinicopathological characteristics such as sex, age, histological differentiation, distant metastasis and lymph node metastasis (p>0.05). There was a significant association with depth of invasion (X2=5.385, p<0.05) and TMN stage (X2=6.255, p<0.05). In gastric carcinoma, positive staining for p53 was detected in 53 of 79 cases (67.1%), showing a significant association with Mina53 (X2=5.161, p<0.05). The mean (+/- SD) PCNA labeling index for gastric carcinoma was 39.47+/-16.92%. Mina53 expression was positively associated with PCNA level (r=0.756, p<0.01). CONCLUSION: Mina53 was overexpressed in gastric carcinoma and associated with tumor proliferation and antioncogene inactivation. Mina53 could therefore play an important role in the carcinogenesis and progression of gastric carcinoma.


Subject(s)
Biomarkers, Tumor/analysis , Nuclear Proteins/analysis , Stomach Neoplasms/chemistry , Adult , Aged , Aged, 80 and over , Dioxygenases , Female , Gastric Mucosa/chemistry , Gastric Mucosa/pathology , Genes, p53 , Histone Demethylases , Humans , Immunohistochemistry , Male , Middle Aged , Nuclear Proteins/physiology , Proliferating Cell Nuclear Antigen/analysis , Stomach Neoplasms/genetics , Stomach Neoplasms/pathology
7.
Shi Yan Sheng Wu Xue Bao ; 32(1): 77-85, 1999 Mar.
Article in Chinese | MEDLINE | ID: mdl-12548798

ABSTRACT

HarpinPss can induce hypersensitive reaction (HR) in tobacco leaves. As superoxide dismutase can inhibit but catalase can not inhibit the development of HR induced by harpinPss, superoxide anion is required for this response. HarpinPss can also induce the release of active oxygen and extracellular alkalinization, two early defence responses in tobacco suspension culture. Diphenylene iodoium, can completely inhibit the induction of HR in tobacco leaves, and the release of active oxygen in the suspension culture system, superoxide anion in these system may be produced by the activation of NADPH oxidase. Ethyleneglycol-bis (beta-aminoethyl) N, N, N'N'-tetraacetic acid (EGTA) can inhibit the development of harpinPss-induced HR and these two early defence responses in suspension culture system. Adding Ca2+ into the medium again, these responses can return to normal level in a short time. Lanthanum chloride, verapamil, neomycin, U-73122, and LiCl can also inhibit these harpinPss-induced responses. Therefore, the influx of Ca2+ mediated by calcium channel and the release of Ca2+ from internal Ca2+ pool may be involved in the two early defense responses induced by harpinPss. Cycloheximide and actinomycin D have no effect on the release of active oxygen but can inhibit harpinPss-induced HR even added them in the intermediate process for inducing HR. It indicates superoxide is just a trigger for HR, and HR is a more complex process that needs the sustained expression of some genes.


Subject(s)
Bacterial Outer Membrane Proteins/pharmacology , Calcium/metabolism , Nicotiana/physiology , Plants, Toxic , Calcium Channels/physiology , Cells, Cultured , Plant Leaves/drug effects , Plant Leaves/physiology , Reactive Oxygen Species/metabolism , Nicotiana/drug effects
8.
Shi Yan Sheng Wu Xue Bao ; 32(1): 88-92, 1999 Mar.
Article in Chinese | MEDLINE | ID: mdl-12548799

ABSTRACT

No matter when anion channel inhibitors, DIDS (4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid) and A9C (anthracene-9-carboxylic acid) added (before, at the same time of or after harpinPss treatment), they can inhibit harpinPss-induced hypersensitive response in tobacco seedlings and release of active oxygen and extracellular alkalinization in tobacco suspension cells. DIDS and A9C also inhibit harpinPss-induced Ca2+ influx. In all these cases, DIDS is more efficient than A9C. It is postulated that anion channel positively regulates calcium channel in plasma membrane, and harpinPss may function through signal transduction mediated by anion channel and calcium channel to regulate cellular Ca2+ concentration and defense responses.


Subject(s)
Bacterial Outer Membrane Proteins/pharmacology , Calcium/metabolism , Ion Channels/physiology , Nicotiana/physiology , Plants, Toxic , Anions , Calcium Channels/physiology , Cells, Cultured , Reactive Oxygen Species/metabolism , Signal Transduction/physiology , Nicotiana/drug effects
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