ABSTRACT
This study evaluated TMAH biodegradation under methanogenic conditions. Under methanogenic conditions, a sludge from a full-scale UASB treating TFT-LCD wastewater was able to degrade 2,000 mg/L of TMAH within 10 h and attained a specific degradation rate of 19.2 mgTMAH/gVSS-h. Furthermore, several chemicals including some surfactants, DMSO, and sulfate were examined for their potential inhibitory effects on TMAH biodegradation under methanogenic conditions. The results indicated that surfactant S1 (up to 2%) and DMSO (up to 1,000 mg/L) presented negligible inhibitory effects on TMAH degradation, while surfactant S2 (0.2-1%) might inhibit methanogenic reaction without any TMAH degradation for 3-5 h. At sulfate concentrations higher than 300 mg/L, a complete inhibition of methanogenic reaction and TMAH biodegradation was observed. Results from cloning and sequencing of archaeal 16S rRNA gene fragments showed that Methanosarcina barkeri and Methanosarcina mazei were the dominant methanogens in the UASB treating TMAH-containing TFT-LCD wastewater.
Subject(s)
Methane/chemistry , Methane/metabolism , Quaternary Ammonium Compounds/chemistry , Quaternary Ammonium Compounds/metabolism , Waste Disposal, Fluid/methods , Water Pollutants, Chemical/chemistry , Methanosarcina/classification , Methanosarcina/genetics , Methanosarcina/metabolism , PhylogenyABSTRACT
The effects of growth interruption times combined with Sb exposure of GaAsSb/GaAs multiple quantum wells (MQWs) have been investigated by using phototransmittance (PT), contactless electroreflectance (CER) and wavelength modulated surface photovoltage spectroscopy (WMSPS). The features originated from different portions of the samples, including interband transitions of MQWs, interfaces and GaAs, are observed and identified through a detailed comparison of the obtained spectra and theoretical calculation. A red-shift of the interband transitions and a broader lineshape of the fundamental transition are observed from samples grown under Sb exposure compared to the reference sample grown without interruption. The results can be interpreted in terms of both increases in Sb content and mixing of Sb in the GaAs interface layers. An additional feature has been observed below the GaAs region in the samples with Sb treatment. The probable origin of this additional feature is discussed.
ABSTRACT
RHI-betaG-PEG, formed by linking poly(ethylene glycol)-modified beta-glucuronidase to Mab RH1, was employed to examine bystander killing of antigen-negative N1S1 rat hepatoma cells by activation of a glucuronide prodrug (BHAMG) of p-hydroxyaniline mustard (pHAM) at antigen-positive AS-30D rat hepatoma cells. Sequential treatment of cells with 10 microg ml(-1) RH1-betaG-PEG and 20 microM BHAMG was not toxic to N1S1 cells but killed 99% of AS-30D cells. Over 98% of N1S1 cells, however, were killed in mixed populations containing as few as 2% AS-30D cells after identical treatment, demonstrating an in vitro bystander effect. Subcutaneous injection of AS-30D and N1S1 cells in BALB/c nu/nu mice produced solid tumours containing both cells. Uptake of radiolabelled RH1-betaG-PEG in solid AS-30D and mixed AS-30D/N1S1 tumours was 11.6 and 9.3 times greater than a control antibody conjugate 120 h after i.v. injection. Intravenous treatment with RH1-betaG-PEG and BHAMG cured seven of seven nude mice bearing solid s.c. AS-30D tumours and significantly delayed, compared with control conjugate and prodrug treatment, the growth of mixed N1S1/AS-30D tumours with one cure, showing that targeted activation of BHAMG kills bystander tumour cells in vivo.
Subject(s)
Aniline Mustard/analogs & derivatives , Antineoplastic Agents, Alkylating/therapeutic use , Glucuronidase/therapeutic use , Immunotoxins/therapeutic use , Polyethylene Glycols/therapeutic use , Prodrugs/therapeutic use , Aniline Mustard/metabolism , Aniline Mustard/therapeutic use , Animals , Antibodies, Monoclonal/metabolism , Antibodies, Monoclonal/therapeutic use , Antineoplastic Agents, Alkylating/metabolism , Diffusion , Drug Screening Assays, Antitumor , Glucuronidase/metabolism , Immunohistochemistry , Immunotoxins/metabolism , Liver Neoplasms, Experimental/metabolism , Liver Neoplasms, Experimental/therapy , Mice , Mice, Inbred BALB C , Mice, Nude , Polyethylene Glycols/metabolism , Prodrugs/metabolism , Rats , Rats, Sprague-Dawley , Time Factors , Tumor Cells, CulturedABSTRACT
Three-dimensional (3D) arrays of digital data representing spatial volumes arise in many scientific applications, such as computed tomography (CT) and magnetic resonance imaging (MRI) created by imaging a series of cross sections of human bodies in medical applications. In this article, a software system architecture, called DISCOVER (a Distributed Interactive Scientific COmputing and Visualization EnviRonment), which can take advantage of the power of parallel processing, is proposed and implemented for interactive visualization and manipulation of the 3D digital data. The surface-rendering and the volume-rendering algorithms are implemented. The same software program can be executed on several different hardware platforms. We also propose a new rendering algorithm, called volume-surface rendering, for medical applications. The algorithm enables users to visualize the external and internal structures of medical objects simultaneously. The network version of the DISCOVER, as it stands today, is in practical use in the Hospital of National Cheng Kung University in Taiwan for real clinical applications.
