ABSTRACT
Angelica sinensis polysaccharides were analyzed using high performance liquid chromatography (HPLC) and Fourier Transform Infrared (FT-IR). The major sugar of the polysaccharide was saccharose (18.55%); and the sugar constituted about 83% of the monomer content. Glucose and fructose were found as minor components of the polysaccharides. The FT-IR spectra of A. sinensis polysaccharides are used for determination of their structural features. The FT-IR spectrum of A. sinensis polysaccharides showed bands at 1641 cm(-1), 1415 cm(-1), 1050 cm(-1) and 926 cm(-1) characteristic for the carboxylic group. Absorptions at 2920-2930 cm(-1) are attributed to asymmetrical stretching vibration of CH(2)-group. Medium stretch observed in the range 1650-1400 cm(-1) is assigned to C-C stretching of polysaccharides. Cardioprotective effects of A. sinensis polysaccharides were evaluated by using myocardial ischemia/reperfusion (IR) rats. A. sinensis polysaccharides treatment significantly reduced myocardial infarction size, enhanced CT-1 and antioxidant enzymes activity, downregulated caspase-12 mRNA expression in rats. The study strongly suggests the cardioprotective activity of A. sinensis polysaccharides in limiting ischemia-reperfusion induced myocardial injury.
Subject(s)
Angelica sinensis/chemistry , Antioxidants/therapeutic use , Polysaccharides/chemistry , Polysaccharides/isolation & purification , Reperfusion Injury/drug therapy , Animals , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/pharmacology , Brain/drug effects , Brain/enzymology , Brain/pathology , Caspase 12/genetics , Caspase 12/metabolism , Catalase/blood , Chromatography, High Pressure Liquid , Cytokines/blood , Gene Expression Regulation, Enzymologic/drug effects , Glutathione/blood , Glutathione Peroxidase/blood , Male , Myocardial Infarction/blood , Myocardial Infarction/complications , Myocardial Infarction/drug therapy , Myocardial Infarction/pathology , Myocardium/enzymology , Myocardium/pathology , Polysaccharides/pharmacology , Polysaccharides/therapeutic use , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reperfusion Injury/blood , Reperfusion Injury/complications , Superoxide Dismutase/bloodABSTRACT
Gas chromatography/mass spectrometry (GC-MS) were used to characterize the polysaccharides in Agaricus brasiliensis. GC-MS analysis showed that the A. brasiliensis polysaccharide was a typical heteropolysaccharide and mainly composed of glucose, arabinose and mannose in the molar percentages of 78.38%, 10.46% and 8.51%, respectively. The Fourier-transform infrared spectra (FT-IR) of A. brasiliensis polysaccharides revealed typical characteristics of polysaccharides. The samples had the characteristics of hydroxyl groups, C-H band and alpha-pyranoses. Ischemia-reperfusion treatment markedly decreased myocardial SOD activity and increased MDA level in rats treated with ischemia-reperfusion. Pharmacological experiment showed that administration of A. brasiliensis polysaccharide could significantly enhance myocardial SOD activity and reduce MDA level in rats treated with ischemia-reperfusion. At the same time, administration of A. brasiliensis polysaccharide could still significantly reduce (p<0.01) caspase-3 level in rats' brain. Results indicated that A. brasiliensis polysaccharide was beneficial in some cardiovascular diseases.
