ABSTRACT
Intracerebral hemorrhage (ICH) is a devastating cerebrovascular associated with inflammation and BBB disruption. Pericytes plays a critical role in neurological diseases, while whether pericytes could be utilized to treat ICH remains to be elucidated. Here, we isolated CD146+CD34- pericytes from rat adipose tissues (ADPs). Fluorescence-activated cells maintained their cell morphology and differentiation potential and expressed pericytes markers (CD146, NG2, and PDGFRß) but not endothelial markers (CD31, CD34, and CD45). ADPs transplantation improved the neuro-behavioral functions in ICH rats and resulted in decreased hematoma volume and neuron loss after ICH. Besides, ADPs graft restrained the infiltration of neutrophils and reactive microgliosis after ICH injury around the peri-hematoma area of rats, as evidenced by increased Iba1- and MPO immunoreactivity. The transplanted pericytes were covered on endothelial cells, and promoted angiogenesis and vascular basement membrane formation in the peri-hematoma area of ICH rats, as shown by double staining of PDGFRß and CD31/CollagenIV. The decreased brain water content and Evans Blue leakage proved the protective role of ADPs graft on BBB permeability. Finally, transplanted ADPs increased the expression of VE-cadherin, ZO-1, and claudin-5, leading to stable endothelial cell-cell adhesion and tight junction. In conclusion, the transplantation of APDs improved neuronal after ICH, which involved different mechanisms including neuroinflammation regulation and BBB dysfunction recovery. Our results supported that ADPs might be the ideal cell type for ICH therapy and provided insights into the potential cell therapy for further ICH treatment.
ABSTRACT
Photothermal conversion behavior has a vital application to disease therapy, water purification, or uncontacted heaters. The fabrication of high-performance photothermal conversion materials especially for near-infrared (NIR) light and microstructures has attracted a great deal of attention. Among numerous substances, MXene as a new type of 2D material with semi-metallic and unique electromagnetic properties presents a broader absorption of light and even a typical plasmonic absorption near the NIR-I area (808 nm), which has made it suitable for photothermal conversion. Here, we propose a facile approach for preparing a Ti3C2Tx/ionic liquid ink with a high photothermal conversion efficiency. The as-prepared ink has showed good wettability of various substrates as well as the high sensitivity of 808 nm NIR light irradiation and a wide range of thermal variation. After packing the ink into a gel pen refill, the flexible thermal chips could be easily obtained just by pen writing on the soft surface with the designed size, which also have become an optimal candidate for the thermal alarm system.
