Clinical value of the expression levels of tumor protein D52 and miR-133a on prognosis assessment of pancreatic cancer surgery.

Objective: To investigate the clinical value of the expression levels of tumor protein D52 (TPD52) and miR-133a on the prognosis assessment of pancreatic cancer surgery. Methods: This was a retrospective study. Ninety-seven patients who underwent radical surgery for pancreatic cancer in Cangzhou Central Hospital from January 2018 to January 2022 were selected and divided into four groups: TPD52 high expression group, TPD52 low expression group, miR-133a high expression group and miR-133a low expression group. The relationship between the expression levels of TPD52 and miR-133a and the clinicopathological features of patients with pancreatic cancer was analyzed. The COX regression model was used to analyze the risk factors affecting the prognosis of patients with pancreatic cancer. Results: The high expression rate of TPD52 and the low expression rate of miR-133a in pancreatic cancer tissues were higher than those in normal paracancerous tissues(P<0.05). Based on the comparison of prognosis and survival, the median survival time of patients with high expression of TPD52 and low expression of miR-133a was lower than that of patients with low expression of TPD52 and high expression of miR-133a, with a statistically significant difference(P<0.05). Moreover, multivariate Cox regression analysis showed that low differentiation of pancreatic cancer, III-IV stage of TNM, high expression of TPD52, as well as low expression of miR-133a were independent risk factors for postoperative survival of patients with pancreatic cancer(P<0.05). Conclusion: TPD52 is expressed at a high level whereas miR-133a at a low level in pancreatic cancer tissues, both of which together with low differentiation of pancreatic cancer and III-IV stage of TNM constitute independent risk factors affecting the surgical prognosis of patients with pancreatic cancer.

Magnetic Resonance Imaging and Serum AFP-L3 and GP-73 in the Diagnosis of Primary Liver Cancer.

Objective: To investigate the combined application value of magnetic resonance imaging (MRI) combined with serum alpha-fetoprotein (AFP)-L3 and Golgi protein (GP)-73 in the diagnosis of primary liver cancer. Methods: The data of 200 patients with suspected liver cancer admitted to our hospital from February 2020 to February 2021 were retrospectively analyzed, and they were randomly divided into an experimental group and a control group, with 100 cases in each group. The experimental group received a combined detection of MRI with serum AFP-L3 and GP-73, and the control group adopted traditional diagnostic methods (spiral computed tomography and serum AFP). The diagnostic yields of the two groups were compared. Surgical resection was performed after the diagnosis of primary liver cancer, and the correlation between the efficacy and combined detection of MRI with serum AFP-L3 and GP-73 levels was analyzed. Results: The two groups presented comparable general information (P >0.05). The surgical results showed 160 cases of primary liver cancer, including 75 cases in the experimental group and 85 cases in the control group, and 40 cases of benign liver lesions. The diagnostic accuracy of the experimental group (73/75, 95%) was significantly higher than that of the control group (76/85, 86%) (P < 0.05). The serum levels of AFP-L3, GP-73, and AFP in patients with primary liver cancer were remarkably decreased after surgery (P < 0.001). The preoperative and postoperative AFP-L3, GP-73, and AFP levels of patients with primary liver cancer were significantly higher than those of patients with benign liver lesions. The AUC (95% CI) for the combined detection of MRI and serum AFP-L3 and GP-73 levels in patients with surgically confirmed primary liver cancer was 0.747 (0.619-0.874). Conclusion: MRI combined with serum AFP-L3 and GP-73 presents favorable diagnostic efficiency in the diagnosis of primary liver cancer, which is worthy of clinical application.

[Distribution Characteristics and Seasonal Variations of Arsenic in Atmospheric Aerosols over the Yellow Sea and Bohai Sea].

Marine aerosol samples of total suspended particulates (TSP) were collected in winter (2017) and spring and summer (2018) over the Yellow Sea and Bohai Sea. These samples were analyzed for total arsenic (As), As(Ⅴ) and As(Ⅲ), and water soluble ions to investigate the distribution and seasonal variation of As in atmospheric aerosols, as well as the dry deposition flux. Results showed that As concentrations in winter, spring, and summer were 6.6, 5.5, and 4.4 ng·m-3, respectively. The highest As concentrations occurred in the winter. Obvious differences in the spatial distribution of As were observed in different seasons. The highest concentrations of As were observed over the Bohai Sea in winter and the northern Yellow Sea in spring, with an average of 8.8 and 11.3 ng·m-3, respectively. As concentrations exhibited a relatively uniform spatial pattern in summer over the Yellow Sea and Bohai Sea, which may have been affected by the different sources of As. As(Ⅴ) was the main species of As, while As(Ⅴ)/As(Ⅲ) ratios were 0.41 in winter and 0.21 in summer, respectively. Average As/TSP ratios in the winter, spring, and autumn were 95.4, 83.9, and 81.4 µg·g-1, respectively. Obviously higher As/TSP ratios, indicating higher intensity, occurred in winter. Air masses carry pollutants released over the Bohai Rim Region by the winter monsoon, resulting in higher As concentrations in winter. In summer, As concentrations are also higher, because air masses bring pollutants to the southeast coastal economic zone via the northeast monsoon. In spring, most air masses arriving in this region originate from Siberia and the southeast ocean with high rate, resulting in lower As concentrations. The significant correlation between K+/TSP and As/TSP (r=0.78, P<0.05) in winter indicates that As is affected by the combustion of land biomass. No significant correlation in summer reveals the difference in As sources between winter and summer. The dry deposition flux of As over the Yellow Sea and Bohai Sea was 1.15 µg·(m2·d)-1 in winter, 0.77 µg·(m2·d)-1 in spring, and 0.97 µg·(m2·d)-1 in summer, with an annual mean value of 0.95 µg·(m2·d)-1.

A temporary stent graft as a new shunt for juxtahepatic vena cava injury.

BACKGROUND: Juxtahepatic vena cava (JHC) injury is difficult to handle because of JHC injury's specific position and liver obstruction. The traditional shunt method initially appeared to be useful but ultimately failed. Simple and effective methods are needed to solve it. METHODS: Firstly, the clinical data, including the computed tomography images, of 120 patients were collected. In addition, a JHC digital model was constructed based on computed tomography images. According to the digital model data, a circulation loop simulating the blood flow in the JHC was established. Secondly, we analyzed the hemodynamics of a JHC shunt with pig blood. Finally, the new shunt was designed based on the data obtained. The shunt consists of a covered stent and transfer device and was tested. RESULTS: The JHC has a three-dimensional cylindrical structure. The mean (SD) length of the retrohepatic vena cava is 78.21 (9.83) mm, which shows correlations with the patient's age and weight (r = -0.343 and 0.271, respectively, p < 0.05). An equation is obtained as follows: retrohepatic vena cava (millimeter) = 71.23 - 0.293 × age (year) + 0.32 × weight (kilogram). The shunt diameters must be 10 mm and 12 mm to maintain the blood pressure difference Point a and Point b at approximately 3.75 mm Hg (5 cm H2O), when the flow rate is 3,000 mL/min and 5,000 mL/min, respectively. The stent graft showed effective hemostasis in tests. However, it failed when the inferior vena cava was pulled harder. CONCLUSION: A temporary stent graft as a new shunt for JHC injury has not been previously reported. It is a combination of both a traditional operation and a simple endovascular technique, which showed effective hemostasis in tests.

[Research on the mercury species in Jiaozhou Bay in spring].

In April 2010, seawater samples collected every twenty minutes in the Jiaozhou Bay were separated and determined in-situ and indoor to study mercury speciation and its daily variation and to further understand the end-result and effect of mercury on offshore environment. Results showed that dissolved element mercury (DEM) concentration of seawater ranged from 38.2 pg x L(-1) to 156 pg x L(-1), with an average value of 97.5 pg x L(-1). The highest and the lowest value appeared at around 13:00 and 17:30 respectively under the influence of tide and light intensity. DEM concentration gradually declined with depth. DEM of surface sea primarily derived from photoreduction of bivalent mercury. Dissolved mercury (DHg) concentrations ranged from 7.32 ng x L(-1) to 49.1 ng x L(-1) (average value was 13.9 ng x L(-1)), from 4.39 ng x L(-1) to 19.3 ng x L(-1) (average value was 7.94 ng x L(-1)) for dissolved reactive mercury (RHg). The maximum peaks of DHg and RHg all appeared around 13:00, due to dirty seawater carried by tidal movement in the lowest tide. The variation trend with depth of RHg and DHg concentrations was similar at different time. Under the influence of the light and water temperature, the ratio of RHg to DHg was higher in the surface water. RHg accounted for 62% of DHg, so the mercury had relatively high activity and biological availability, and contributed to the form of DEM. The methylmercury concentration was low, with an average value of 0.30 ng x L(-1), and some samples were lower than the detection limit.