Integrated multilevel investigation of photosynthesis revealed the algal response distinction to differentially charged nanoplastics.

Nanoplastics (NPs), especially those with different charges, as one of emerging contaminants pose a threat to aquatic ecosystems. Although differentially charged NPs could induce distinct biological effects, mechanistic understanding of the critical physiological processes of aquatic organisms from an integrated multilevel perspective on aquatic organisms is still uncertain. Herein, multi-effects of differentially charged nanosized polystyrene (nPS) including neutral nPS, nPS-COOH, and nPS-NH2 on the photosynthesis-related physiological processes of algae were explored at the population, individual, subcellular, protein, and transcriptional levels. Results demonstrated that both nPS and nPS-COOH exhibited hormesis to algal photosynthesis but nPS-NH2 triggered severe inhibition. As for nPS-NH2, the integrity of algal subcellular structure, chlorophyll biosynthesis, and expression of photosynthesis-related proteins and genes were interfered. Intracellular NPs' content in nPS treatment was 25.64 % higher than in nPS-COOH treatment, and the content of chloroplasts in PS and nPS-COOH treatment were 3.09 % and 4.56 % higher than control, respectively. Furthermore, at the molecular levels, more photosynthesis-related proteins and genes were regulated under nPS-COOH exposure than those exposed to nPS. Light-harvesting complex II could be recognized as an underlying explanation for different effects between nPS and nPS-COOH. This study first provides a novel approach to assess the ecological risks of NPs at an integrated multilevel.

Physicochemical properties of nanosized biochar regulated by heat treatment temperature dictates algal responses: From the perspective of fatty acid metabolism.

Nanosized biochar (NBC) is an important fraction of biochar (BC) as it can exert nano-scale effects on aquatic organisms, attracting increasing research attention. However, effects of different physicochemical properties of NBC on biological responses at the metabolic and gene expression level are not comprehensively understood. Here, biological effects of NBCs pyrolyzed at different heat treatment temperatures (HTTs, 350-700 °C) were evaluated using freshwater algae Chlorella vulgaris, from the perspectives of growth and fatty acid (FA) profile changes. NBC pyrolyzed at 700 °C (N700) induced the greatest algal growth inhibition and oxidative stress than N350 and N500. In addition, NBC exposure to 50 mg/L increased saturated and monounsaturated FAs, along with a decrease in polyunsaturated FAs (PUFAs). Exposure to NBC also significantly influenced the expression of key FA metabolism genes (3fad, sad, kasi and accd), demonstrating the potential role of reactive oxygen species-mediated PUFA reduction accompanied by increased membrane permeability in algal toxicity upon NBC exposure. The observed differences in response to N700 were attributed to its smaller particle size and higher abundance of -COOH. These findings reveal the underlying mechanisms in the algal response to NBCs and provide valuable guidance for the safe design and application of BC materials.

Insights into eco-corona formation and its role in the biological effects of nanomaterials from a molecular mechanisms perspective.

Broad application of nanotechnology inevitably results in the release of nanomaterials (NMs) into the aquatic environment, and the negative effects of NMs on aquatic organisms have received much attention. Notably, in the natural aquatic environment, ubiquitous ecological macromolecules (i.e., natural organic matter, extracellular polymeric substances, proteins, and metabolites) can easily adsorb onto the surfaces of NMs and form an "eco-corona". As most NMs have such an eco-corona modification, the properties of their eco-corona significantly determine the fate and ecotoxicity of NMs in the natural aquatic ecosystem. Therefore, it is of great importance to understand the role of the eco-corona to evaluate the environmental risks NMs pose. However, studies on the mechanism of eco-corona formation and its resulting nanotoxicity on aquatic organisms, especially at molecular levels, are rare. This review systemically summarizes the mechanisms of eco-corona formation by several typical ecological macromolecules. In addition, the similarities and differences in nanotoxicity between pristine and corona-coated NMs to aquatic organisms at different trophic levels were compared. Finally, recent findings about potential mechanisms on how NM coronas act on aquatic organisms are discussed, including cellular internalization, oxidative stress, and genotoxicity. The literature shows that 1) the formation of an eco-corona on NMs and its biological effect highly depend on both the composition and conformation of macromolecules; 2) both feeding behavior and body size of aquatic organisms at different trophic levels result in different responses to corona-coated NMs; 3) genotoxicity can be used as a promising biological endpoint for evaluating the role of eco-coronas in natural waters. This review provides informative insight for a better understanding of the role of eco-corona plays in the nanotoxicity of NMs to aquatic organisms which will aid the safe use of NMs.