Subject(s)
Chorioretinitis/diagnosis , Endophthalmitis/diagnosis , Eye Infections, Bacterial/diagnosis , Legionellosis/diagnosis , Retinal Detachment/diagnosis , Chorioretinitis/microbiology , Coloring Agents , Diagnosis, Differential , Electroretinography , Endophthalmitis/microbiology , Eye Infections, Bacterial/microbiology , Female , Fluorescein Angiography , Humans , Indocyanine Green , Legionella/pathogenicity , Legionellosis/microbiology , Middle Aged , Retinal Detachment/microbiologyABSTRACT
OBJECTIVE: Bietti crystalline dystrophy is a rare form of tapetoretinal degeneration associated with retinal crystalline deposits. However, Bietti crystalline dystrophy is extremely unusually associated with macular hole formation. A 32-year-old man with Bietti crystalline dystrophy and bilateral macular holes is described. DESIGN: Case report and literature review. RESULTS: Clinical and angiographic features, optical coherence tomography results, electroretinographic findings, and visual evoked potentials are reported. CONCLUSION: Bietti crystalline dystrophy can occur with bilateral macular holes, but the cause is unclear.
Subject(s)
Antiviral Agents/administration & dosage , Corneal Ulcer/drug therapy , Eye Infections, Viral/drug therapy , Gentamicins/adverse effects , Needlestick Injuries/diagnosis , Retina/drug effects , Retina/injuries , Retinal Diseases/diagnosis , Adult , Anti-Bacterial Agents/adverse effects , Anti-Bacterial Agents/therapeutic use , Blindness/chemically induced , Blindness/diagnosis , Corneal Ulcer/virology , Drug Therapy, Combination , Eye Injuries, Penetrating/diagnosis , Eye Injuries, Penetrating/drug therapy , Fluorescein Angiography , Gentamicins/therapeutic use , Humans , Injections/adverse effects , Male , Needlestick Injuries/drug therapy , Ophthalmic Solutions/administration & dosage , Retinal Diseases/chemically inducedABSTRACT
PURPOSE: To investigate the effect of antisense oligonucleotides (ODN) to Nogo-A mRNA expression in oligodendrocytes and to establish the base for further research of repair of optic nerve injury. METHOD: (1) Oligodendrocytes were obtained by inoculating the optic nerve of newborn (2 days) rats and were identified with galactocerebroside(GC) antibody immunocytochemical stain. (2) In order to observe the effects of antisense ODN on cultured cells, we set up five groups, including the groups of three concentration of antisense Nogo-A ODN (2 microM, 5 microM, 10 microM), a group with the random sequence added to the medium and the control group. Reverse transcription-polymerase chain reaction (RT-PCR) was adopted to study the effects of ODN on the expression of Nogo-A in oligodendrocytes. RESULTS: (1) Three days after inoculation, a few of round or fusiform shape cells migrated from optic nerve tissue; About 11 days later, the coverlips were completely covered by the cells; The cells identified with GC antibody immunocytochemical stain were positive cells. (2) The result of RT-PCR study showed that antisense Nogo-A ODN could significantly and specifically inhibit the expression of Nogo-A after 24 hours (P < 0.01). Random sequence has no effect on Nogo-A expression. CONCLUSION: Antisense Nogo-A ODN can effectively and specifically inhibit the expression of Nogo-A.
