A Novel Repair Idea on Nasal Sidewall Defect in Asians: Local Tissue Regeneration.

BACKGROUND: The traditional ways indicate using different types of flap to repair the nasal sidewall defect, but the scar in the donor area or bloated flap is a problem that cannot be ignored. This study propose the clinical application of the principle of tissue regeneration priority in small defects (defect diameter <1.0 cm) of nasal sidewall. METHODS: A retrospective study of 3 patients experienced the tumor excision and tissue regeneration healing in situ from January 2019 to January 2020. In this group, the nasal sidewall defect was treated with wound moist theory, preventing infection, to promote the wound regeneration and repair. RESULT: The longest follow-up time was 8 month, all patient undergone half a year follow-up at least. One of them were left small sunken scars but all of them healed up well and satisfied with the results. CONCLUSION: When dealing with the small defect of the skin and soft tissue on the nasal sidewall (defect diameter <1.0 cm), this article provided a new idea that is application of the priority principle for tissue regeneration repair rather than traditional flap used for 1-stage repair. For the small defects of the skin and soft tissue on the nasal sidewall, the tissue regeneration and natural repair in situ can achieve satisfactory effects. More importantly, it has the advantages of simplicity, ease of operation, and fewer complications.

[Detection Rate of Central Nervous System Leukemia Can Be Improved by Cell Preservation Solution].

OBJECTIVE: To investigate whether cell preservation solution can prolong the survival time of leukemia cells and increase the survival rate, so as to improve the detection rate of central nervous system leukemia. METHODS: Kasumi cells were added into cerebrospinal fluid (CSF) supernatant with or without cell preservation solution to compare cell viability and biological characteristics at different time point. Wright Giemsa staining was used to compare cell morphology; cell counting, CCK-8 method, and trypan blue staining were used to compare the cell number, and flow cytometry was used to compare the cell viability. The expression of AML-ETO tumor fusion gene was detected by fluorescence quantitative RT-PCR. RESULTS: At different time points (8 h and 24 h), the survival, molecular biological characteristics and RT-PCR result of the cells in CSF with cell preservation solution were significantly better than those in normal cerebrospinal fluid. CONCLUSION: Cell preservation solution can effectively improve the survival time and survival rate of leukemic cells, thereby increase the detection rate of CNS leukemia.

Human amnion-derived mesenchymal stem cells promote osteogenic and angiogenic differentiation of human adipose-derived stem cells.

Tissue engineering using suitable mesenchymal stem cells (MSCs) shows great potential to regenerate bone defects. Our previous studies have indicated that human amnion-derived mesenchymal stem cells (HAMSCs) could promote the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). Human adipose-derived stem cells (HASCs), obtained from adipose tissue in abundance, are capable of multi-lineage differentiation. In this study, the effects of HAMSCs on osteogenic and angiogenic differentiation of HASCs were systematically investigated. Proliferation levels were measured by flow cytometry. Osteoblastic differentiation and mineralization were investigated using chromogenic alkaline phosphatase activity (ALP) activity substrate assays, Alizarin red S staining, real-time polymerase chain reaction (real-time PCR) analysis of osteogenic marker expression, and Western blotting. We found that HAMSCs increased the proliferation and osteoblastic differentiation of HASCs. Moreover, enzyme-linked immunosorbent assay (ELISA) and human umbilical vein endothelial cells (HUVECs) tube formation suggested HAMSCs enhanced angiogenic potential of HASCs via secretion of increased vascular endothelial growth factor (VEGF). Thus, we conclude that HAMSC might be a valuable therapeutic approach to promote HASCs-involved bone regeneration.