ABSTRACT
Allicin is a sulfur-containing compound extracted from raw garlic (Allium sativum L.). We compared the effect of allicin addition on growth performance, serum biochemical parameters, and rumen microbiota of goats compared to monensin. Twenty-four Anhui white goats were assigned randomly to one of three dietary treatments: 1) a basal diet (CON); 2) the basal diet with allicin addition at 750 mg per head per day (AC); 3) the basal diet with monensin addition at 30 mg per kg of diet (MS). Animals were fed for 8 weeks. Results showed the average daily gain, and feed efficiency was increased with allicin and monensin addition. Serum levels of IgG, total superoxide dismutase, and glutathione peroxidase were higher in the AC group than those in the CON and MS groups. The microbiota analysis revealed that monensin addition mainly affected genera related to carbohydrate and protein metabolism, and allicin mainly affected genera related to energy metabolism and intestinal health. In conclusion, allicin could improve growth performance and have advantages over monensin in improving the antioxidant capacity and immune function of goats. Allicin may be a potential alternative to monensin.
Subject(s)
Disulfides , Garlic , Microbiota , Sulfinic Acids , Animals , Animal Feed/analysis , Antioxidants/metabolism , Diet/veterinary , Dietary Supplements/analysis , Goats/metabolism , Monensin/pharmacology , Rumen/metabolismABSTRACT
PURPOSE: Many studies have evaluated the association between the HLA-DRA rs3129882 A/G polymorphism and risk for Parkinson's disease (PD) in Chinese-based populations, however, published data remain inconclusive. Therefore, we performed a meta-analysis from all relevant studies to evaluate an association of HLA-DRA rs3129882 A/G polymorphism with susceptibility to PD. METHODS: The summary odds ratio (OR) with its 95% confidence interval (CI) was calculated to evaluate the association. The Q statistic was used to evaluate homogeneity and funnel plots were used to assess publication bias. The minor A allele frequencies, additive, dominant as well as recessive genetic models were examined in the analyses. RESULTS: Five case-control studies with a total of 2230 PD cases and 2262 controls from Mainland China, Taiwan, Singapore, and Malaysia were included in the final meta-analysis. Neither the minor A allele frequencies nor the genotypic distributions were significantly different between PD cases and controls when all studies were pooled into this meta-analysis. CONCLUSION: The results of this meta-analysis suggest that HLA-DRA rs3129882 A/G polymorphism was not responsible for PD in Chinese-based populations.
Subject(s)
Genetic Predisposition to Disease/genetics , HLA-DR alpha-Chains/genetics , Parkinson Disease/genetics , Polymorphism, Single Nucleotide/genetics , Asian People , Community Health Planning , Databases, Bibliographic/statistics & numerical data , Gene Frequency , Genotype , Humans , Risk FactorsABSTRACT
Fibroblast growth factor 20 (FGF20) is a neurotrophic factor which enhances the survival of rat midbrain dopamine neurons. Genetic variation in FGF20 may influence the risk of occurrence and development in Parkinson's diseases (PD). Many studies have evaluated the association between FGF20 rs1721100 C/G polymorphism and the risk of sporadic PD; however, published data are still controversial. The aim of the present meta-analysis was to evaluate the association of FGF20 rs1721100 C/G polymorphism with susceptibility of PD. The summary odds ratio (OR) with its 95 % confidence interval (CI) was calculated to estimate the association. Five case-control studies with a total of 3,463 sporadic PD cases and 4,606 controls were finally included into this meta-analysis. Neither the basic allele frequencies nor the genotypic distributions of rs1721100 C/G within FGF20 were different between two groups when all studies were pooled into the meta-analysis. Subgroup analysis by ethnicity showed FGF20 rs1721100 C/G polymorphism was significantly associated with increased risk in the heterozygote comparison model (CG versus GG: OR = 0.83, 95 % CI, 0.72-0.95, P = 0.009) in Asians but not in Caucasians. Overall, this meta-analysis suggests that FGF20 rs1721100 C/G polymorphism is associated with sporadic PD in Asians.
Subject(s)
Fibroblast Growth Factors/genetics , Genetic Predisposition to Disease , Parkinson Disease/genetics , Polymorphism, Single Nucleotide , Asian People/genetics , Humans , Publication Bias , White People/geneticsABSTRACT
Embryonic stem cells (ESCs)-based therapies have been increasingly recognized as a potential tool to replace or support cells and their function damaged by the neurodegenerative process that underlies Parkinson's disease (PD). In this study, we implanted engineered mouse embryonic stem (ES) CGR8 cells, which stably co-express glial cell line-derived neurotrophic factor (GDNF) and tyrosine hydroxylase (TH), into striatum (Str) or both Str and substantia nigra (SN) of parkinsonian rats lesioned by 6-hydroxydopamine (6-OHDA). We found that cell transplantation into Str or both Str and SN rescued behavioral abnormalities and striatal DA depletion associated with 6-OHDA lesion. Our findings suggested that the profound functional impairment in nigrostriatal circuitry could be at least partially restored by ESCs-based expression of TH and GDNF, which may be developed into a useful tool for PD therapy.
Subject(s)
Embryonic Stem Cells/transplantation , Glial Cell Line-Derived Neurotrophic Factor/metabolism , Oxidopamine/metabolism , Parkinson Disease/therapy , Tyrosine 3-Monooxygenase/metabolism , Animals , Cell Line , Cell Survival , Corpus Striatum/metabolism , Disease Models, Animal , Female , Genetic Therapy , Glial Cell Line-Derived Neurotrophic Factor/biosynthesis , Glial Cell Line-Derived Neurotrophic Factor/genetics , Mice , Neuroprotective Agents/metabolism , Neurotoxicity Syndromes/metabolism , Neurotoxicity Syndromes/therapy , Parkinson Disease/metabolism , Rats , Rats, Wistar , Substantia Nigra/cytology , Substantia Nigra/metabolism , Tyrosine 3-Monooxygenase/biosynthesis , Tyrosine 3-Monooxygenase/geneticsABSTRACT
The effects of myricetin on 6-hydroxydopamine (6-OHDA)-induced neurodegeneration in the substantia nigra-striatum system were investigated. By high-performance liquid chromatography electrochemical detection, we showed that the dopamine content in the striatum decreased after 6-OHDA treatment, which could be restored by myricetin. The immunohistochemistry and semiquantitative reverse transcription-PCR studies showed that myricetin could prevent the 6-OHDA-induced decrease of tyrosine hydroxylase positive neurons and the tyrosine hydroxylase mRNA expression in the substantia nigra. Perls' iron staining study further demonstrated that myricetin prevented the 6-OHDA-induced increase of iron-staining cells in the substantia nigra. These results suggested that the protective effects of myricetin on the toxicity of 6-OHDA could be attributed to the myricetin-suppressed iron toxicity.
Subject(s)
Flavonoids/therapeutic use , Nerve Degeneration/chemically induced , Nerve Degeneration/drug therapy , Oxidopamine , Animals , Brain Chemistry/drug effects , Chromatography, High Pressure Liquid/methods , Disease Models, Animal , Dopamine/metabolism , Electrochemistry/methods , Female , Gene Expression Regulation/drug effects , Nerve Degeneration/pathology , RNA, Messenger/biosynthesis , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction/methods , Substantia Nigra/drug effects , Substantia Nigra/metabolism , Substantia Nigra/pathology , Tyrosine 3-Monooxygenase/metabolismABSTRACT
AIM: To investigate antimalarial mechanism of Qinghaosu ( QHS) and its derivatives. METHODS: The electronic structure of QHS and its derivatives were completely optimized and calculated at B3LYP/6-31G * level, while the route was at HF/STO-3G level. RESULTS: The peroxide bridge is the active center of QHS and induced by ferrous iron to produce cyclic product. CONCLUSION: Heme can link with QHS derivatives.
