ABSTRACT
The influence of coexisting copper (Cu) ion on the degradation of pesticides pyrethroid cypermethrin and cyhalothrin in soil and photodegradation in water system were studied. Serial concentrations of the pesticides with the addition of copper ion were spiked in the soil and incubated for a regular period of time, the analysis of the extracts from the soil was carried out using gas chromatography (GC). The photodegradation of pyrethroids in water system was conducted under UV irradiation. The effect of Cu2+ on the pesticides degradation was measured with half life (t0.5) of degradation. It was found that a negative correlation between the degradation of the pyrethroid pesticides in soil and Cu addition was observed. But Cu2+ could accelerate photodegradation of the pyrethroids in water. The t0.5 for cyhalothrin extended from 6.7 to 6.8 d while for cypermethrin extended from 8.1 to 10.9 d with the presence of copper ion in soil. As for photodegradation, t0.5 for cyhalothrin reduced from 173.3 to 115.5 min and for cypermethrin from 115.5 to 99.0 min. The results suggested that copper influenced the degradation of the pesticides in soil by affecting the activity of microorganisms. However, it had catalyst tendency for photodegradation in water system. The difference for the degradation efficiency of pyrethroid isomers in soil was also observed. Copper could obviously accelerate the degradation of some special isomers.
Subject(s)
Copper/chemistry , Nitriles/chemistry , Pyrethrins/chemistry , Chromatography, Gas , Molecular Structure , Pesticides/chemistry , Photochemistry/methods , Soil/analysis , Soil Pollutants/chemistry , Water Pollutants, Chemical/chemistry , Water Pollutants, Chemical/radiation effectsABSTRACT
In order to detect the permethrin (Py) residue in environment samples, Ovalbumin(OVA)was used as protein carrier to couple with semi-antigen permethrin by active ester method. Indirect competitive ELISA (icELISA) was established. The most appropriate titration of coating antigen was 0.45 microg/mL and optimal dilution was 1/2 500 correspondingly. The standard curve of icELISA was also established and the curve indicated that the lowest detection limit was 0.l microg/mL, the curve had a favorable linear relation with the concentration range of 10 to approximately 800 microg/mL, recoveries of permethrin (>97%) were satisfactory.
