Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 5 de 5
Filter
Add more filters










Database
Publication year range
2.
J Immunol ; 181(10): 7356-66, 2008 Nov 15.
Article in English | MEDLINE | ID: mdl-18981159

ABSTRACT

Despite steady progress in elimination of measles virus globally, measles infection still causes 500,000 annual deaths, mostly in developing countries where endemic measles strains still circulate. Many adults are infected every year in China, with symptoms more severe than those observed in children. In this study, we have used blood samples from adult measles patients in Shanghai and age-matched healthy controls to gain an understanding of the immune status of adult measles patients. IFN-alpha mRNA was reduced in patient PBMC compared with healthy controls. In contrast, gene expression and plasma production of IL-2, IL-10, and IFN-gamma were elevated in patient blood. A similar cytokine profile was observed at early times when cultured PBMC were infected with a clinical isolate of measles virus. In contrast to previous studies in pediatric patients, we did not find a reduction in total CD4(+) and CD8(+) T cells in patient PBMC. Interestingly, we found that CD4(+)CD25(+)CD127(low) regulatory T cells were significantly increased in patient PBMC compared with controls. Using intracellular cytokine staining we also show that the measles virus induces IL-10-producing CD14(+) and CD4(+)CD25(+) cells in PBMC. Our results show that adult measles patients in the acute phase of the disease have a mixed Th1/Th2 type response, accompanied with severe immunosuppression of both innate and adaptive responses including suppression of type I IFN. Both regulatory T cells and plasma IL-10 may contribute to the immunosuppression.


Subject(s)
Interleukin-10/biosynthesis , Measles/immunology , T-Lymphocytes, Regulatory/immunology , Adult , Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay , Female , Flow Cytometry , Gene Expression , Humans , Interferon-gamma/biosynthesis , Interferon-gamma/immunology , Interleukin-2/biosynthesis , Leukocytes, Mononuclear/immunology , Lipopolysaccharide Receptors/immunology , Lipopolysaccharide Receptors/metabolism , Macrophages/immunology , Macrophages/metabolism , Male , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction
3.
Zhonghua Gan Zang Bing Za Zhi ; 15(10): 734-7, 2007 Oct.
Article in Chinese | MEDLINE | ID: mdl-17963597

ABSTRACT

OBJECTIVE: To evaluate the clinical effect and side-effect of peg-interferon alfa-2a (PEG-IFN alfa-2a) and highly active antiretroviral therapy (HAART) for patients infected with hepatitis C virus (HCV) and co-infected with human immunodeficiency virus (HIV). METHODS: Twenty-two patients with HCV/HIV co-infection received highly active antiretroviral therapy initially; after their CD4 lymphocyte counts rose to over 0.20x10(9)/L, they were separated into two groups: one group with CD4 lymphocytes over 0.35x10(9)/L (high group) and one group with CD4 lymphocytes below 0.35x10(9)/L (low group). Both groups were given 180 microg of PEG-IFN alfa-2a every week intramuscularly. HCV RNA and HIV RNA loads, blood cell and CD4 lymphocyte counts, and liver functions were routinely examined. RESULTS: After 12, 24 and 48 weeks of PEG-IFN alfa-2a therapy, mean HCV RNA loads reduced 2.0650 log10 copies/ml (t=3.8733), 2.9146 log10 copies/ml (t=7.6741) and 2.4315 log10 copies/ml (t=5.8202) from the baseline at week 0 in the 13 patients in the high group, and reduced 1.1522 log10 copies/ml (t = 2.8937), 1.4189 log10 copies/ml (t=2.4422) and 1.1167 log10 (t=1.1261) in the 8 patients of the low group. However, there was no significant difference between the early viral response rate (EVR) and the end of treatment viral response rate (ETVR) of the two groups. In the high group, the white blood cell count was lower at 24 weeks than the base line (t=2.4700), and the blood platelet count was lower both at 24 and 48 weeks than the base line (t=2.3273 and t=3.6149). CONCLUSIONS: PEG-IFN alfa-2a can effectively reduce HCV RNA loads in patients with HCV-/HIV co-infection, and the inhibition rate in patients with higher CD4 lymphocyte counts is better. The EVR and ETVR of the two groups of patients show similar results after the treatment. PEG-IFN alfa-2a can reduce the white blood cell counts and the blood platelet counts in the peripheral blood.


Subject(s)
Acquired Immunodeficiency Syndrome/drug therapy , Antiretroviral Therapy, Highly Active , Hepatitis C/drug therapy , Interferon-alpha/therapeutic use , Polyethylene Glycols/therapeutic use , Superinfection/drug therapy , Acquired Immunodeficiency Syndrome/virology , Adult , CD4 Lymphocyte Count , HIV-1 , Hepacivirus , Hepatitis C/virology , Humans , Interferon alpha-2 , Male , Middle Aged , RNA, Viral , Recombinant Proteins , Superinfection/virology , Viral Load , Young Adult
4.
Mycoses ; 50(6): 475-80, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17944709

ABSTRACT

Invasive fungal infections (IFIs) have become a major cause of morbidity and mortality among people with acquired immune deficiency syndrome (AIDS), however, little is known about the clinical features and prognosis of IFI in AIDS in China. This study aimed to characterise the clinical features and prognosis of IFI in AIDS patients in China. We retrospectively reviewed the records of all HIV-infected patients at a Chinese university hospital between December 2004 and May 2006. We identified 35 patients with IFI. IFIs included thrush, oesophageal candidiasis, fungal pneumonia, cryptococcosis, penicilliosis and fungaemia, 44.4% of IFIs occurred in the digestive tract, 71.8% of IFIs occurred in patients with CD4(+)T-lymphocyte counts <100 cells mm(-3). Candida albicans accounted for 57.4% of fungal pathogens isolated. All the patients received both antiretroviral and antifungal therapy; 27 patients were cured and eight died. IFI is one of the most common opportunistic infections in AIDS patients in China. IFIs mainly occur in patients with low CD4(+)T-lymphocyte counts. The majority of IFIs occur in the digestive tract. The most common pathogen causing IFI is C. albicans. The mortality rate remains high although antiretroviral therapy and many newer antifungals are available in China.


Subject(s)
AIDS-Related Opportunistic Infections/epidemiology , Acquired Immunodeficiency Syndrome/complications , Hospitals, University , Mycoses/epidemiology , Mycoses/microbiology , AIDS-Related Opportunistic Infections/microbiology , Acquired Immunodeficiency Syndrome/drug therapy , Adult , Aged , Antifungal Agents/therapeutic use , Antiretroviral Therapy, Highly Active , Candida/classification , Candida/isolation & purification , Candida albicans/isolation & purification , Candidiasis, Oral/drug therapy , Candidiasis, Oral/epidemiology , Candidiasis, Oral/microbiology , China , Cryptococcosis/drug therapy , Cryptococcosis/epidemiology , Cryptococcosis/microbiology , Cryptococcus neoformans/isolation & purification , Female , Fungemia/drug therapy , Fungemia/epidemiology , Fungemia/microbiology , Humans , Incidence , Male , Middle Aged , Mycoses/drug therapy
5.
Zhongguo Zhen Jiu ; 27(12): 892-4, 2007 Dec.
Article in Chinese | MEDLINE | ID: mdl-18271230

ABSTRACT

OBJECTIVE: To observe the therapeutic effect of moxibustion on AIDS patients of spleen-kidney yang-deficiency. METHODS: Sixty-six cases of AIDS were divided into a treatment group and a control group, 33 cases in each group. All of the patients were treated with HAART, with moxibustion at Tianshu (ST 25), Shenque (CV 8), Zhongwan (CV 12), Guanyuan (CV 4) added in the treatment group for 3 months. Clinical symptoms and cell immunity were recorded before and after treatment. RESULTS: After treatment, the effective rate was 90.9% in the treatment group, which was better than 66.7% in the control group (P < 0.05). The improvement of the score for clinical symptoms in the treatment group was superior to that in the control group (P < 0.01). After treatment, the CD4 lymphocyte counts increased in the two groups, with no significant difference between the two groups (P > 0.05). Additionally, increase of total lymphocyte count in the treatment group was superior to that in the control group (P < 0.05). CONCLUSION: Moxibustion can increase the therapeutic effect of HAART on AIDS patients and increase the total lymphocyte count.


Subject(s)
Acquired Immunodeficiency Syndrome/therapy , Moxibustion/methods , Yang Deficiency/therapy , Acquired Immunodeficiency Syndrome/immunology , Adult , Antiretroviral Therapy, Highly Active , Female , Humans , Kidney/physiopathology , Male , Medicine, Chinese Traditional , Middle Aged , Spleen/physiopathology , Yang Deficiency/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...