ABSTRACT
BACKGROUND: A simple and reproducible model of hepatectomy provides an essential basis for the study of liver regeneration. However, current rodent models of hepatectomy involve lobectomy, which cannot simulate clinical liver resection with surgical margins. The main purpose of this study was to evaluate a novel murine modeling technique for hepatectomy using a gutta cutter. METHODS: Seventy-five C57BL/6 mice were randomly divided into 3 groups (n = 25 mice per group). Group 1 (control) underwent single ligature of the left lobe. Group 2 underwent left lobe local (5-mm diameter) hepatectomy by gutta cutter. Group 3 underwent partial left lobe resection (1.5 cm) by gutta cutter. Postoperative complications were analyzed. Serum aspartate transaminase, alanine aminotransferase, alkaline phosphatase, urea nitrogen, interleukin 6, and tumor necrosis factor-α were detected using an automatic biochemical analyzer. Hematoxylin-eosin and immunohistochemical staining was used to examine pathology, proliferating cell nuclear antigen, caspase-3, CD34, signal transducer and activator of transcription 3 (STAT-3), and phosphorylated STAT-3 (p-STAT-3). RESULTS: Major postoperative complications, hepatic enzymes, kidney function, interleukin 6, and tumor necrosis factor-α were similar among the groups (all P > .05). Histology showed little necrosis and a clear surgical boundary in groups 2 and 3. Groups 1 and 3 had higher positive cell levels (proliferating cell nuclear antigen, CD34, p-STAT-3) than group 2 (P < .05). There were no significant differences in caspase-3 and STAT-3 positive cells. CONCLUSIONS: Hepatectomy in mice using a gutta cutter to better mimic human liver resection shows potential as an alternative and safe animal model. This model may be useful in investigating methods of promoting liver regeneration.
Subject(s)
Hepatectomy , Interleukin-6 , Alanine Transaminase , Animals , Caspase 3 , Feasibility Studies , Hepatectomy/adverse effects , Hepatectomy/methods , Humans , Liver/pathology , Liver/surgery , Liver Regeneration , Mice , Mice, Inbred C57BL , Postoperative Complications/surgery , Proliferating Cell Nuclear Antigen , Tumor Necrosis Factor-alphaABSTRACT
Tissue engineered bone brings hope to the treatment of bone defects, and the osteogenic differentiation of stem cells is the key link. Inducing osteogenic differentiation of stem cells may be a potential approach to promote bone regeneration. In recent years, lncRNA has been studied in the field increasingly, which is believed can regulate cell cycle, proliferation, metastasis, differentiation and immunity, participating in a variety of physiology and pathology processes. At present, it has been confirmed that certain lncRNAs regulate the osteogenesis of stem cells and take part in mediating signaling pathways including Wnt/ß-catenin, MAPK, TGF-ß/BMP, and Notch pathways. Here, we provided an overview of lncRNA, reviewed its researches in the osteogenic differentiation of stem cells, emphasized the importance of lncRNA in bone regeneration, and focused on the roles of lncRNA in signaling pathways, in order to make adequate preparations for applying lncRNA to bone tissue Engineering, letting it regulate the osteogenic differentiation of stem cells for bone regeneration.
