ABSTRACT
OBJECTIVE: To compare early-term effects of totally laparoscopic distal gastrectomy with delta-shaped anastomosis (D-STLDG) with conventional laparoscopic-assisted distal gastrectomy (LADG). METHODS: Clinical data of 24 patients who received D-STLDG from April 2013 to April 2014, and 45 patients who received LADG from March 2010 to December 2012 were retrospectively analyzed. The operative time, intra-operative blood loss, post-operative recovery time of intestinal function, post-operative pain, the length of post-operative hospital stay and the incidence of post-operative complications (infection, obstruction and delayed gastric emptying) were compared between the two groups. RESULTS: All procedures were completed successfully and all patients of both groups were discharged smoothly from hospital. Compared with LADG, D-STLDG had shorter operative time (175.3±64.7 min vs. 205.8±42.2 min, P<0.05), less intra-operative blood (50.8±25.3 ml vs. 75.2±22.5 ml, P<0.05), shorter post-operative recovery time of intestinal function (1.2±0.5 d vs. 2.1±0.8 d, P<0.05), less post-operative pain (5.6±0.7 vs. 7.8±0.5, P<0.05), shorter post-operative hospital stay (8.5±2.2 d vs. 10.5±3.5 d, P<0.05). There were no significant difference in surgical margins achieved, the number of lymph nodes retrieved or the incidence of post-operative complications (infection, obstruction and delayed gastric emptying) (P>0.05). CONCLUSION: The delta-shaped anastomosis of reconstructing the digestive tract in TLDG appears to be safe, feasible and associated to faster recovery.
ABSTRACT
OBJECTIVE: To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC. METHODS: SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method. RESULTS: Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group (P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29±0.07, significantly lower than that in paracancer group and normal group (P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus (P<0.05). CONCLUSIONS: Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.