[Morphine decreases the voltage sensitivity of the slow sodium channels]. / Morfine umen'shaet chuvstvitel'nost' k potentsialu medlennykh natrievykh kanalov.

Morphine was shown to decrease in a dose-dependent manner the effective charge transfer in tetrodotoxin-resistant (TTXr) sodium channel activation system in short-term cultured dorsal root ganglion cells. Morphine seems to interact with opioid receptors because of total block of the binding by naloxone and naltrexone. Neither activating, nor inhibiting G-protein agents exerted any effect on this process. The morphine signal was blocked by extracellular application of 2 x 10(-4) M ouabain. The findings suggest existence of sodium signalling pathway involving receptors, Na+, K(+)-ATPase and the TTXr sodium channels.

[The effect of activated macrophage products on neurite growth in an organotypic culture of chick embryo sensory ganglia]. / Vliianie produktov aktivirovannykh makrofagov na rost neiritov v organotipicheskoi kul'ture chuvstvitel'nykh gangliev kurinykh émbrionov.

Murine peritoneal macrophages, activated by BCG vaccine, and human peripheral blood monocytes, activated by lipopolysaccharides, exerted neurite stimulating or neurite inhibiting effects in various periods of activation. The supernatants of these preparations were active in organotypic culture of chick embryo dorsal root ganglia. The inhibition of neurite growth on the 1st day of cultivation was followed by the neurite-stimulating effect. The fluctuation of neurite-inhibition and neurite-stimulation effect of macrophage supernatants suggest the availability of certain changes in cytokine composition in different periods of macrophage activation.

[The effect of veratridine on the release of neurotrophic factors in a nerve tissue culture]. / Vliianie veratridina na vydelenie neirotroficheskikh faktorov v kul'ture nervnoi tkani.

A depolarising agent veratridine was shown to affect the level of neurotrophic substances in a combined culture of the newborn rats hippocampus and chick embryo spinal ganglia: the growth of sprouts of the ganglia's sensitive neurons was enhanced. The effect seems to be mediated by the veratridine effect on the hippocampal neurons. The role of an increased level of neurotrophic factors in an increase of neuronal activity, is discussed.

[The protective effect of the nerve growth factor on sensory neurons in organotypic culture under the action of diphtheria toxin]. / Protektiruiushchii éffekt faktora rosta nervov na chuvstvitel'nye neirony v organotipicheskoi kul'ture pri deistvii na nee difteriinogo toksina.

Diphtheria toxin (1.10(-1) - 1.10(-6) Lf/ml) was found to inhibit neurite extension in chick embryo dorsal root ganglia in vitro. If the nerve growth factor (60 ng/ml) was added with the toxin to culture media, the effect of diphtheria toxin was seen to decrease and the neurite outgrowth was compared with control. Protective effect of nerve growth factor estimated by the influence of diphtheria toxin may be used for new principles of diphtheria treatment.

[The stimulating effect of prolactin on the neurite growth of sensory neurons in an organotypic culture]. / Stimuliruiushchee vliianie prolaktina na rost neiritov chuvstvitel'nykh neironov v organotipicheskoi kul'ture.

Prolactine was found to promote neurite extension in chick embryo dorsal root ganglia in vitro. This hormone was active at the concentration 10-50 ng/ml provided extensive neurite outgrowth in the bioassay, compared to the control. The liquor from patients with prolactinoma was also found to promote neurite extension. This fact may be used for prolactinoma diagnostics.