[Markers of inflammation--monocyte chemoattractant protein-1 (MCP-1) and C-reactive protein--in blood of patients with unstable angina pectoris and stable effort angina].

AIM: To estimate concentrations of C-reactive protein (CRP) and MCP-1 in blood plasma of patients with unstable angina (UA) and stable effort angina (SEA). MATERIAL AND METHODS: Multiprojection coronaroangiography was performed in 12 patients with UA and 11 patients with SEA. Hemodynamically significant stenosis (50% and more) at least in one major coronary artery was confirmed in all the patients. CRP and MCP-1 were measured with latex agglutination and enzyme immunoassay (Biosource kits), respectively. RESULTS: UA patients had significantly higher plasma levels of MCP-1 and CRP than those with SEA (107.25 +/- 16.19 vs. 63.0 +/- 16.16 pg/ml and 1.99 +/- 1.64 vs 0.44 +/- 0.28 mcg/ml, respectively). CONCLUSION: Estimation of MCP-1, as a marker of vascular wall inflammation, can be used, in line with other indices, for verification of UA.

[Nicotine effect on cell immunity in rats with adjuvant induced monoarthritis].

The effect of subchronic nicotine consumption on cell immunity in rats with adjuvant-induced monoarthritis and in intact animals was investigated. In intact animals, nicotine intake was associated with increase of absolute T-cell number and T-cell proliferative response to mitrogen stimulation in vitro, the latter being not due to the altered lymphocyte sensitivity to glucocorticoids. Nicotine intake by rats with arthritis resulted in significant expansion of T-helper and cytotoxic cells and decrease of natural killers in blood.

[Potential influence of nicotine on inflammation and induction of autoantibodies in rats with adjuvant arthritis]. / Potentsiruiushchee deistvie nikotina na vospalinie i induktsiiu autoantitel u krys.

The influence of chronic administration of nicotine diluted in the drinking water on the parameters of systemic inflammation and autoimmune processes in rats (August line) with adjuvant-induced arthritis, were studied. The experiments have shown that nicotine acts as an antiphlogistic means (the amount of C-reactive protein rises in the blood) and activates autoimmune processes: induction of rheumatic factor, of autoantibodies to serotonin, and glial fibrillar acid protein. It was supposed that nicotine has a potential impact on immunocompetent cells.

[Markers of inflammation, autoantibodies to neurospecific antigens and outcome in patients with acute ischemic stroke]. / Markery vospaleniia, autoantitela k neirospetsificheskim antigenam i kharakter iskhoda u bol'nykh s ostrym ishemicheskim insul'tom.

To identify biochemical markers for carotid stroke outcome, blood serum levels of inflammation markers (C-reactive protein, orosomucoid, soluble p-selectin) and autoantibodies (AAB) to neurospecific antigens (glial fibrillary acidic protein, neuron specific enolase, S-100 protein) were studied in 27 patients (mean age 64 +/- 6 years) with acute ischemic stroke in inner carotid artery system on day 1-2 and 21 of the disease onset. To day 21, patients with good rehabilitation of neurological functions (group 1) demonstrated a decrease of C-reactive protein and soluble p-selectin concentrations, and unfavorable disease course was associated with a significant (p<0.05) increase of concentrations of these indices. On day 1 and 7, a level of AAT to glial fibrillary acidic protein was higher (p<0.05) in group 1 than in that with minimal rehabilitation and to day 21 it decreased relatively the baseline level. At the same time, patients with minimal rehabilitation had a stable AAT level. On day 7, the AAT level correlated with expression of neurological deficit on day 21 (r=0.510; p=0.019). No stroke-course-dependent differences were found in dynamics of orosomucoid as well as of AAT to neuron specific enolase and S-100 protein levels.

[Possibility of selective binding of HIV-1 infected cells by immunosorbents]. / Izuchenie vozmozhnosti selektivnogo sviazyvaniia infitsirovannykh VICh-1 kletok s pomoshch'iu immunosorbentov.

A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.

[Immunosorption of individual HIV proteins and virions]. / Immunosorbtsiia individual'nykh bel'kov i virionov VICh.

Immunosorbents specifically binding native (gp160, gp120, gp41) and recombinant env proteins and HIV-I virions were synthesized on the basis of Sepharose 4B and Silica with immobilized ligands such as gamma-fraction of rabbit antiserum to HIV-I proteins and purified antibodies to env proteins of HIV-I. The possibility was shown of selective extraction of HIV-I virions and individual HIV proteins both in vitro and in vivo. The titer of virus antigens (in ELISA) after perfusion via an immunosorbent of patterns with a high content of virions and HIV-I proteins was 8 times as low as the starting titer (after perfusion via the control sorbent it was 2-fold decreased). Extracorporeal immunosorption in animals after intravenous injection of recombinant env protein permitted the latter's titer to be 5 times lower. After perfusion via the control sorbent the titer dropped by at least 20% as compared with the starting titer. The possibility of using immunosorption in multimodality therapy of AIDS is under discussion.

[Leukocyte thermostable alpha globulin immunochemical test in the evaluation of the functional state of human leukocytes]. / Immunokhimicheskii test na LTAG dlia otsenki funktsional'nogo sostoianiia leikotsitov cheloveka.

For detection of functional State of Human Leucocytes incubation test was proposed. From the levels of LTAG (Leucocyte thermostable alpha-globulin)--one of the cationic proteins of granules neutrophils, which excrete neutrophils from granules in the time of incubation, it is possible to estimate the degree of injury of the functional state of Leucocytes from patients and in experimental systems in vitro. The immunochemical techniques were used to study the levels of LTAG.

[The use of siliceous immunosorbents in hemosorption]. / Ispol'zovanie kremnezemnykh immunosorbentov v gemosorbtsii.

Experiments were conducted on rabbits and monkeys for approbation of immunosorbents by covalent immobilization of human serum albumin or antibodies to human apoprotein B on the surface of silo-chromium and subsequent incubation with 1% albumin solution. Extracorporeal immunosorption in immunized rabbits led to reduction of the level of specific antibodies by 60% and the number of blood platelets by 10-30%. The initial antibody level was restored in 5-10 days. Study of immunosorbents on monkeys showed a satisfactory compatibility of the sorbent with blood, its ability for regeneration, and the possibility of its repeated use. The specific capacity was 66% of the capacity according to human apoprotein B.