[Effect of age and gender on genotoxic and biochemical indexes in animal blood after low doses of radiation-mercury exposures].

The study was executed with the young (4-5 months) and elderly (15-18 months) rats of both gender at the experiment start. The mercury salt was administered into animals with drinking water in 1 mcg/l concentration during 3 months before and 1 month after single X-irradiation with 25 cGy dose. The genotoxic effects in leukocytes were evaluated on the base of the content and structure DNA alterations in 24 hrs and in 1 month after irradiation. The alaninaminotransferase activity and the urea content in serum were determined to evaluate functional status of liver and kidney, respectively. The irradiation of rats receiving mercury with drinking water resulted in increase of the leukocyte DNA content in young male and decrease of the leukocyte quantity in young female at the early terms. At the same time the decrease of monocyte content and the alterations of the blood leukocyte DNA structure were observed in the elderly female. One month after combined exposures the whole leukocyte amount in young females was increased to the intact control level and the leukocytosis was observed in elderly females accompanied by significant condensation of the leukocyte DNA structure. At the same time granulocyte quantity was observed in young males in compare with the intact rats, and the tendency of the increased genotoxicity was lasted out. After combined exposures the functional liver alterations were observed in the highest degree among young male rats (+62%), in approximately identical degree among young and elderly females (+38% and +27%), and practically were absent among elderly males. The absence of the urea concentration increase in the serum of the all experimental group animals in comparison with the intact control rats evidenced, that lesion of kidneys did not take place. So, in despite of the fact that young females revealed sensitivity to the radiation-mercury environment exposure with the low doses in the early term, then they demonstrated the better restoration in compare with the young males and the elderly females. The elderly males were found the least sensitive to the radiation-mercury exposure with low doses among the studied animal groups.

[Effects of ligfol after radiation-mercury exposure in rats of various age].

The study was carried out with the young (4-5 months) and elderly (15-18 months) female and male rats at the experiment start. The mercury salt was administered into animals with drinking water in 1 mcg/l concentration during 3 months before and 1 month after single X-irradiation with 25 cGy dose. The genotoxic effects in leukocytes were evaluated on the base of the content and structure DNA alterations in 24 hrs and in 1 mth after irradiation. The alaninaminotransferase activity in serum was determined to evaluate functional status of liver. A protector intramuscular injection of Ligfol preparation with the 250 mg/kg dose in 24 hrs before irradiation resulted in increase of the blood leukocyte amount, more expressed in 1 mth after irradiation. The standardizing effect of preparation on the leukocyte DNA content is manifested in 1 mth after combined exposure on rats within young male and elderly female. At the same time, Ligfol showed some restoring effect on the alterations of leukocyte supercoiled DNA structure in 1 mth after irradiation within elderly female rats. The preparation showed the standardizing effect on the liver cells in young males injured by radiation-mercury exposures with low doses, however Ligfol had not influence practically on the destruction degree of hepatocytes after similar injury within female young and elderly rats. So, results of the intramuscular Ligfol injection is manifested at least during 1-2 mths; the young rats of both gender and elderly females showed the most sensitivity to the radiation-mercury exposure with low doses and to the Ligfol, according to the studied indexes.

[Effect of selenium on reactions of elderly rats affected by low doses of radiochemical exposures]. / Vlianie selena na reaktsii pozhilykh krys pri vozdeistvii malykh doz radiatsionno-khimicheskikh faktorov.

The 18-months-old female rats received with drinking water Hg2(NO3) in concentration of 1 mkg/l in account to metal and a preparation of selenium "Neoselen" (Na2SeO2) in concentration of 1.3 ml/kg/day, respectively, during 1 month before and 1 month after single gamma-irradiations with 25 cGy doses. Are established early (through 24 h after an irradiation) cytologic and genotoxic reactions in leukocytes of blood, and also hematological, biochemical reactions and changes of the content and frame DNA of leukocytes of blood in 30 day after an irradiation? Features of action of low doses of radiation in a combination to ions of mercury and their combination with a preparation of selenium are revealed. Communication of postradiating effects--changes of indexes of DNA of leukocytes of blood with reduction of life expectancy of animals is shown. The data are received, permissive to draw the conclusion, that the preparation of selenium in utilised concentrations, essentially not influencing on lifetime at low doses of radiation--mercury affecting is capable to improve quality of life of elderly animals

[Morphofunctional characteristics of the immunocompetent system in hypotrophy and its correction by thymogen]. / Morfofunktsional'naia kharakteristika immunokompetentnoi sistemy pri gipotrofii i ee korrektsii timogenom.

Administration of the synthetic thymic peptide-thymogen to the pigs with symptoms of hypotrophy exerted a positive effect on the course of this pathological condition this resulting from the stimulation of the central and peripheral parts of the immunogenesis. 230 days after the treatment the positive effect still persisted.