ABSTRACT
Two continuous human glioma-derived cell lines, UC-11MG and UC-302MG were established in our laboratory. Both cell lines persistently showed cytologic features similar to those of their respective original tumors. UC-11MG expressed glial fibrillary acidic protein (GFAP) and S-100 protein. The cell lines were negative for factor VIII related antigen (FVIII/RAg) and positive for fibronectin and neuronal specific enolase (NSE). Electron microscopic studies of UC-11MG revealed intermediate filament; filopodia and pinocytic vesicles were present in both lines. Dibutyryl cAMP (dB-cAMP) caused inhibition of growth and marked shift in the morphology of UC-302MG toward spindle cells. The cytologic appearance of UC-11MG treated with dB-cAMP was altered less, but cells showed a stronger GFAP expression, with 'cable' formation. Doubling time was 41.0 +/- 6.4 hours for UC-11MG and 43.7 +/- 6.6 hours for UC-302MG. The karyotypes of both cell lines were aneuploid with chromosomal derangement and markers characteristic for each line.
Subject(s)
Glioma/pathology , Bucladesine/pharmacology , Cell Division/drug effects , Cell Line , Chromosome Mapping , Culture Techniques , Glial Fibrillary Acidic Protein/analysis , Glioma/genetics , Histocytochemistry , Humans , Karyotyping , Male , Microscopy, Electron , Middle Aged , Organ Culture Techniques , S100 Proteins/analysis , Theophylline/pharmacologyABSTRACT
Four cases of radiation-associated gliomas are described. All patients were white men, irradiated in childhood for craniopharyngioma, anaplastic ependymoma, retinoblastoma of the orbit, and Burkitt's lymphoma, respectively. The dose ranged from 1800 to 5900 rads, and the latency period was 5 to 25 years. All primary and secondary tumors were verified histologically, and no evidence of persistence of the primary tumors was found. All secondary tumors arose in the fields of irradiation. Ninety-six cases of radiation-induced tumors of the central nervous system have been reported in the literature to date. Twenty-four were gliomas and occurred mainly in young men.
Subject(s)
Brain Neoplasms/pathology , Brain/radiation effects , Glioma/pathology , Neoplasms, Multiple Primary/pathology , Neoplasms, Radiation-Induced/pathology , Adolescent , Adult , Astrocytoma/pathology , Brain/pathology , Brain Neoplasms/radiotherapy , Child , Child, Preschool , Combined Modality Therapy , Follow-Up Studies , Glioblastoma/pathology , Humans , Infant , Infant, Newborn , Male , Radiotherapy Dosage , Tomography, X-Ray ComputedABSTRACT
We describe a case of a granular cell tumor (GCT) of the suprasellar region with an 11-year history in a 26-year-old woman. The computed tomographic scan showed a midline, contrast-enhancing, noncalcified mass. The biopsy was diagnosed as GCT. The tumor was treated with radiation therapy. At necropsy, a large, homogeneous GCT surrounded by gliosis was found. The tumor cells were filled with granules positive for periodic acid-Schiff, diastase-resistant. The cells did not contain glial fibrillary acidic protein or S-100 protein. Electron microscopy showed tumor cells filled with innumerable lysosomal structures. No intermediate filament was found within the cytoplasm. The tumor cells were not surrounded by a basement membrane. Based on this study and on our review of the literature, the suggestion that GCT has a multicellular origin is upheld.
