Genotype × region and genotype × production level interactions in Holstein cows.

The identification of the presence of genotype by environment interaction effects on important traits in Holstein cattle allows for the use of international genetic evaluations and a more efficient design of regional genetic evaluation programmes. The aim of this study was to determine the genotype × environment interaction effects in Chilean Holstein dairy cattle through the analysis of records corresponding to calvings between 1998 and 2015. Herds were classified in the central and southern regions of Chile based on herd location as well as by high and low levels of production environments based on the fat plus protein yield averages per herd within each region. The central region has a Mediterranean climate and a confined production system while the southern region has a humid temperate climate and a production system based on grazing with supplementation. Traits studied were milk yield (MY), fat yield (FY), protein yield (PY), fat content (FC) and protein content (PC) by lactation, age at first calving (AFC) and calving interval (CI). Several four-trait mixed animal models were applied to environmental category data as different traits, which included herd-year-calving season (herd-year-birth season for AFC) and lactation number as fixed effects, and animal additive genetic, sire-herd, permanent environment and residual effects as random effects. Genetic correlations (rg) for MY, FY, FC, PC and CI were found to decrease as differences between environmental categories increased. The rg between the most extreme environmental categories considered in this study for AFC (0.26) was the only one found statistically lower than 0.60. Genetic correlation values statistically lower than 0.80 (P < 0.05) were observed for AFC, CI, MY, FY and PY between some environmental categories. If separate genetic evaluations are adopted as practical criteria when the value of rg is lower than 0.60, the consequence of improving a multi-trait economic breeding objective in this population is likely to be small unless extreme environmental categories are considered. However, a moderate decrease in selection response and re-ranking of selection candidates is expected for AFC, CI and yield traits when selection is performed in different environmental conditions. Genotype × environment interaction effects involving production systems in a Mediterranean climate and confinement vs. Temperate Oceanic climate and grazing with supplementation, and between two fat plus protein yield level categories within each environment, were at most moderate for the studied traits.

Cirugía ambulatoria electiva en patología proctológica: experiencia prospectiva de 14 años en un hospital público docente / Elective ambulatory surgery in proctological pathology: 14 years of prospective experience in a public teaching hospital

INTRODUCCIÓN: Se presenta nuestra experiencia en cirugía ambulatoria en patología proctológica. MATERIALES Y MÉTODO: La serie corresponde al análisis de 1.399 pacientes tratados entre agosto de 2003 y diciembre de 2017 en forma prospectiva, RESULTADOS: Fueron intervenidos por fístula anorrectal (20%), enfermedad hemorroidaria (19%), enfermedad pilonidal sacro coccígea (EPSC) (15%), fisura anal (13%), biopsia rectal quirúrgica o resección local endoanal (12%), condilomas (10%) y otras (10%). La morbilidad inmediata es de un caso, por un hematoma luego de una cirugía por EPSC que requirió hemostasia y cierre primario. La tasa de hospitalización inmediata fue de 0,3% y corresponde a 5 casos de retención aguda de orina. La hospitalización tardía fue de un 1,6% y corresponde a 22 pacientes, hospitalizados por sangrado tardío (9), dolor intratable (9) y fiebre (4). Todos fueron tratados en forma conservadora con resolución entre los 2 y 5 días. No se registra morbilidad mayor en esta serie. CONCLUSIONES: La cirugía ambulatoria en patología proctológica es factible y segura.

INTRODUCTION: We present our prospective experience in ambulatory anorectal surgery between August 2003 and December 2017. MATERIALS AND METHOD: The series corresponds to the analysis of 1399 patients treated between August 2003 and December 2017 prospectively. RESULTS: The etiology of the surgerys were anal fistula (20%), hemorrhoidal disease (19%), sacrococcygeal pilonidal disease (15%), anal fissure (13%), rectal surgical biopsy or local resection (12%), condylomata (10%) and others (10%). The immediate morbidity was seen one case, a hematoma after an EPSC surgery that required hemostasis and primary closure. The immediate hospitalization rate was 0.3% and corresponds to 5 cases of acute urinary retention. The late hospitalization was 1.6% and corresponds to 22 patients, due to late bleeding (9), severe pain (9) and fever (4). All were treated conservatively with resolution between 2 and 5 days. No major morbidity is recorded in this series. CONCLUSION: We concluded that outpatient surgery in proctologic pathology is feasible and safe.

Tratamiento de la enfermedad hemorroidaria con desarterialización y pexia guiada por doppler (THD®): experiencia inicial / Treatment of haemorrhoidal disease with desarterialization and fixing guided by Doppler

Resumen Introducción: La enfermedad hemorroidaria puede ser tratada de múltiples formas. Una alternativa es la ligadura de las ramas terminales de la arteria rectal y pexia de los paquetes guiados por Doppler (THD®). Objetivo: Presentar nuestra experiencia inicial con esta técnica. Material y Método: Serie prospectiva, consecutiva, no aleatoria. Incluye a los pacientes tratados por enfermedad hemorroidaria que no responde al manejo no quirúrgico. Resultados: La serie corresponde a 11 pacientes, 7 de género masculino. Todos fueron operados en forma ambulatoria. El tiempo quirúrgico promedio fue de 35 min y la evaluación del dolor posoperatorio inmediato no superó un EVA de 3 en todos los pacientes. El seguimiento promedio es de 12 meses y solo un paciente requirió una ligadura con banda elástica por sangrado hemorroidario. Conclusión: La técnica de ligadura y pexia guiada por Doppler es sencilla, con buenos resultados iniciales y con mínimo dolor posoperatorio.

Introduction: Hemorrhoidal disease can be treated by many surgical options. One of them are Transanal Haemorroidal Dearterialization (THD®). Objective: To present our initial experience with this technique. Material and Methods: Prospective, consecutive, non-random series. It includes patients treated for haemorrhoidal disease unresponsive to no operative management. Results: The series consists of 11 patients, 7 male gender. All were operated on an outpatient basis. The average operating time was 35 minutes and the immediate post-operative pain assessment did not exceed an AVS of 3 in all patients. The average follow up was 12 month. Only one patient needed a rubber band ligation for hemorrhoidal bleeding control. Conclusion: The THD technique is simple, with good initial results and minimal post-operative pain.

Performance da equipe do Barcelona e seus adversários nos jogos finais da Champions League e da Copa do Mundo de Clubes FIFA 2010 / Rendimiento del equipo de Barcelona y sus oponentes en la final de los partidos de la Liga de Campeones y la Copa Mundial de Clubes de la FIFA 2010 / Performance of Barcelona's team and their opponents in the finals matches of the Champions League and the FIFA Club World Cup 2010

Objetivo: O presente estudo tem como objetivo avaliar a performance da equipe do Barcelona em confronto com seus adversários, nos dois jogos mais importantes da temporada (final do Mundial Interclubes da FIFA e final UEFA Champions League), entre Barcelona e Santos FC, e entre Barcelona e Manchester United, respectivamente. Método: A análise dos comportamentos desempenhados pelos jogadores levou em consideração o número de jogadores envolvidos e os fundamentos técnicos divididos em três categorias: fundamentos ofensivos, fundamentos defensivos e tipos de passe. Resultados: A partir da análise dos jogos, foi possível observar a superioridade da equipe do Barcelona, em ambos os jogos, onde a mesma tende a ficar mais tempo com a posse de bola e envolver uma maior quantidade de jogadores na fase ofensiva. Conclusão: O modelo de jogo evidenciado privilegia a manutenção sistemática da posse de bola, caracterizando um ataque posicional de abertura e redução de espaços de jogo

Objetivo. El presente estudio tiene como objetivo evaluar el rendimiento del equipo en la confrontación del Barcelona con sus oponentes, en los dos partidos más importantes de la temporada (finales de la Copa Mundial de Clubes FIFA y la UEFA Champions League), entre Barcelona y Santos FC, y entre Barcelona y Man-chester United, respectivamente. Método. El análisis de las conductas realizadas por los jugadores ha tenido en cuenta el número de jugadores involucrados y los fundamentos técnicos divididos en tres categorías: los fundamentos ofensivos, fundamentos defensivos y tipos de pases. Resultados. A partir del análisis de los juegos, fue posible observar la superioridad del equipo de Barcelona en los dos partidos, en los que tiende a tener más tiempo el balón y hacer participar a un mayor número de jugadores en la fase ofensiva. Conclusión. El modelo de juego evidenciado favorece el mantenimiento sistemático del balón, caracterizando un ataque posicional de apertura del juego y reducción de los espacios de juego (AU)

Objective. The present study aims to evaluate the performance of the team in Barcelona confrontation with their opponents, the two most important games of the season (end of the FIFA World Club Cup and UEFA Champions League Final), between Barcelona and Santos FC, and between Barcelona and Manchester United respectively. Method. The analysis of the behaviors performed by players took into account the number of players involved and the technical fundamentals divided into three categories: offensive fundamentals, defensive fundamentals and types of passes. Results. From the analysis of the games, it was possible to observe the superiority of the Barcelona team in both games, when they tend to spend more time with the ball and involve a greater number of players in the offensive phase. Conclusion. The game model evidenced favors systematic maintenance of possession, featuring a positional attack and reduced opening game spaces (AU)

Diferencias clínicas entre adolescentes hospitalizados por intento suicida y adolescentes hospitalizados por otra causa psiquiátrica / Clinical differences between suicide and non suicide attempter hospitalized adolescents

Background: Suicide is the third cause of death among adolescents in United States and between 5 to 12 percent of teenagers have a history of suicide attempt. Therefore, suicide is a public health problem in this age group. Aim: To compare the clinical characteristics of hospitalized adolescents who attempted suicide with those without a history of suicide attempt. Material and Methods: The clinical records of adolescents aged less than 18years, hospitalized in a psychiatric clinic, were analyzed. Forty six patients aged 15 ±2years (72 percent females) had a history of suicide attempt and were considered cases and 81 patients aged 15 ±2 years (52 percent females) did not have a history of suicide attempt and were considered controls. Demographical and clinical characteristics of both groups were compared. Psychiatric diagnoses were based on the Diagnostic and Statistical Manual of Mental Disorders IV (DSMIV). Results: No significant differences were found between groups in the length of hospital stay. The most common psychiatric diagnosis was depressive disorder, found in 71 and 35 percent of suicide and non-suicide attempters, respectively (p < 0.01). Personality diagnosis was deferred in most studied cases. Conclusions: Depression is more common among hospitalized adolescents who attempt suicide.

Conización leep en NIE de alto grado en una institución privada de salud / Leep conization for high degree intraepithelial neoplasias

Antecedentes: La conización cervical del cuello uterino es considerada el tratamiento de elección de las neoplasias intraepiteliales de alto grado. Objetivo: Analizar los resultados del tratamiento de esta patología mediante conización Leep. Método: Se revisan los antecedentes y biopsias de 145 pacientes con diagnóstico histológico de neoplasia intraepitelial II y III tratadas con conización cervical por asa Leep en la Unidad de Patología del Tracto Genital Inferior de Clínica Dávila desde el 1 de junio de 2002 al 31 de mayo de 2008. Resultados: Hubo una correlación colpo-biópsica de 80 por ciento (116/145) para Lie de alto grado. Hubo borde positivo en 75 pacientes (51,7 por ciento) y negativo en 70 (48,3 por ciento). Fue necesario un segundo tratamiento en 5 pacientes (3,4 por ciento); en 3 casos por recidivas y en 2 por lesiones invasoras. El 89 por ciento permanece en seguimiento, comprobándose que la totalidad de estas ha tenido un tratamiento considerado satisfactorio. Conclusión: Nuestros resultados confirman que las lesiones cervicales de alto grado deben ser tratadas por métodos escisionales preferentemente, siendo la conización con asa Leep el método de elección por su facilidad de realización, bajo costo de insumos y puede ser realizado sin grandes requerimientos de infraestructura.

Background: The cervical conización of the uterine cervix is considered the treatment of election of high degree intraepithelial neoplasias. Objective: To analyze the results of treatment of this pathology by Leep Conization. Method: The antecedents and biopsies of 145 patients with histological diagnosis of intraepithelial II and III neoplasia, treated with cervix Conization Leep at Lower Genital Tract Unit of Davila Clinic from June first 2002 to May 31 2008 are reviewed. Results: There was a colpo-biopsy correlation of 80 percent (116/145) for high degree CIN. There was positive edge in 75 patients (51.7 percent) and negative in 70 (48.3 percent). A second treatment in 5 patients were necessary (3.4 percent); in 3 cases by recurrences and 2 by invasive lesions. 89 percent remain in control, verifying itself that the totality of these has had a satisfactory considered treatment. Conclusion: Our results confirm that the high degree cervical lesions must preferably be treated by excision methods, being the Leep Conization the method of election by their facility of accomplishment, low cost and can be made without great infrastructure requirements.

Optical coherence tomography characteristics of full-thickness traumatic macular holes.

PURPOSE: The objective of this paper is to describe the optical coherence tomography (OCT) characteristics of patients with full-thickness traumatic macular hole (TMH) and to correlate them with biomicroscopy findings. METHODS: Twelve eyes of ten consecutive patients with full-thickness TMH participated in this observational retrospective multicentre study. Patients underwent biomicroscopic fundus examination, colour fundus photography, and OCT. RESULTS: Traumatic macular hole was documented with OCT in five women and five men. Mean (range) time between trauma and macular hole (MH) diagnosis was 8.1 (1-24) months. The shape of TMHs was round in 11 (91.7%) eyes. The posterior vitreous was completely detached in six (50%) eyes, and with an operculum in one (8.3%) eye. The common findings seen on OCT were: (1) full-thickness loss of retinal tissue through the hole with sharp edges, perpendicular to the retinal pigment epithelium in five (41.7%) eyes; (2) TMH with an operculum totally detached from the hole's edge in two (16.7%) eyes; (3) presence of epiretinal membrane around of the hole in three (25%) eyes; and (4) presence of abnormalities of the surrounding retina in all (100%) eyes. The OCT characteristics correlated well with biomicroscopic findings, and these characteristics may be predictive for final visual acuity (VA) in TMHs. Only one of the TMHs closed spontaneously in our series. CONCLUSION: Optical coherence tomography complements biomicroscopy in the evaluation of full-thickness TMHs.

[Clinical characteristics of children and adolescent hospitalized in a university psychiatric clinic]. / Características clínicas de niños y adolescentes menores de 18 años hospitalizados en una clínica psiquiátrica universitaria.

BACKGROUND: Five percent of children and adolescents consults in mental health services in one year. Approximately one every ten children has a mental health problem. AIM: To assess clinical and demographic factors of children and adolescents hospitalized by psychiatric cause in a university psychiatric clinic. PATIENTS AND METHODS: Review of medical records of 167 subjects aged 9 to 17 years, 97 women, admitted to a Psychiatric Service in the period 2001-2004. The International Classification of Primary Care (ICPC) manual of the World Organization of National Colleges, Academies and Academic Associations of General Practitioners/Family Physicians (WONCA) was used to classify admission complaints and symptoms and DSMIV to classify psychiatric disorders. RESULTS: Mean hospital stay was 11+8 days. The main admission causes were suicidal attempts and psychomotor agitation/impulsive behavior in 54% and 26% of cases, respectively. The main psychiatric diagnoses were depressive disorder, suicide attempt and bipolar disorder. In 69% of patients, the personality diagnosis was deferred. Only 25% of families were considered functional. CONCLUSIONS: Affective disorders and suicidal behavior are the main psychiatric diagnoses at discharge in children and adolescents admitted to a psychiatric impatient service.

Características clínicas de niños y adolescentes menores de 18 años hospitalizados en una clínica psiquiátrica universitaria / Clinical characteristics of children and adolescent hospitalized in a University Psychiatric Clinic

Blackground: Five percent of children and adolescents consults in mental health services in one year. Approximately one every ten children has a mental health problem. Aim To assess clinical and demographic factors of children and adolescents hospitalized by psychiatric cause in a university psychiatric clinic. Patients and Methods: Review of medical records of 167 subjects aged 9 to 17 years, 97 women, admitted to a Psychiatric Service in the period 2001-2004. The International Classification of Primary Care (ICPC) manual of the World Organization of National Colleges, Academies and Academic Associations of General Practitioners/Family Physicians (WONCA) was used to classify admission complaints and symptoms and DSMIV to classify psychiatric disorders. Results: Mean hospital stay was 11+8 days. The main admission causes were suicidal attempts and psychomotor agitation/impulsive behavior in 54 percent and 26 percent of cases, respectively. The main psychiatric diagnoses were depressive disorder, suicide attempt and bipolar disorder. In 69 percent of patients, the personality diagnosis was deferred. Only 25 percent of families were considered functional. Conclusions: Affective disorders and suicidal behavior are the main psychiatric diagnoses at discharge in children and adolescents admitted to a psychiatric impatient service.

Programa de transtornos de alimentación en adolescentes y jóvenes chilenos: variaciones a seis meses de tratamiento / Multidisciplinary treatment of eating disorders in Chilean adolescents and young adults: Six months outcome

Background: Eating disorders may have serious organic consequences derived from under nutrition, specific nutrient deficiencies and electrolytic disturbances and reach a mortality as high as 12 percent. Aim: To describe the features and outcome after six months of treatment of patients attending the Eating Disorders Unit at the Catholic University Hospital in Chile. Material and methods: Review of medical records of patients with eating disorders that received a multidisciplinary treatment by a team of nutritionists, psychiatrists and psychologists and were seen at least twice in a period of six months. Results: The records of 81 patients (mean age 16.3±3 years, only one male) were included in the review. Forty nine patients had Anorexia Nervosa of the restricting type, five of the purging type, 22 had Bulimia Nervosa of the purging type and two of the non purging type and three patients had an eating disorder not otherwise specified. Eighty percent consulted within the first year of symptom appearance. Sixty five percent came from families with both parents living together, 57 percent had a rigid and agglutinated family structure and 65 percent had occult crises. Depression or dysthymia was found in 45 percent of patients and drug therapy was required at the beginning of treatment in 25 percent. Obsessive traits (40.4 percent) were significantly related to restrictive eaters. A past history of obesity or overweight was common. After six months of treatment, body mass index increasing significantly in restrictive eaters with obsessive traits and occult family crises was noticed. Conclusions: A multidisciplinary therapeutic intervention improved body mass index and family symptoms in patients with restrictive eating disorders.

Enhanced generation of specific tumor-reactive CTL in vitro by selected Melan-A/MART-1 immunodominant peptide analogues.

The Melan-A/MART-1 gene, which is expressed by normal melanocytes as well as by most fresh melanoma samples and melanoma cell lines, codes for Ags recognized by tumor-reactive CTL. HLA-A*0201-restricted Melan-A-specific CTL recognize primarily the Melan-A(27-35) (AAGIGILTV) and the Melan-A(26-35) (EAAGIGILTV) peptides. The sequences of these two peptides are not necessarily optimal as far as binding to HLA-A*0201 is concerned, since both lack one of the dominant anchor amino acid residues (leucine or methionine) at position 2. In this study we introduced single amino acid substitutions in either one of the two natural peptide sequences with the aim of improving peptide binding to HLA-A*0201 and/or recognition by specific CTL. Surprisingly, analogues of the Melan-A(27-35) peptide, which bound more efficiently than the natural nonapeptide to HLA-A*0201, were poorly recognized by tumor-reactive CTL. In contrast, among the Melan-A(26-35) peptide analogues tested, the peptide ELAGIGILTV was not only able to display stable binding to HLA-A2.1 but was also recognized more efficiently than the natural peptide by two short-term cultured tumor-infiltrated lymph node cell cultures as well as by five of five tumor-reactive CTL clones. Moreover, in vitro generation of tumor-reactive CTL by stimulation of PBMC from HLA-A*0201 melanoma patients with this particular peptide analogue was much more efficient than that observed with either one of the two natural peptides. These results suggest that the Melan-A(26-35) peptide analogue ELAGIGILTV may be more immunogenic than the natural peptides in HLA-A*0201 melanoma patients and should thus be considered as a candidate for future peptide-based vaccine trials.

[Experience of a single center in liver transplantation in adults and children]. / Experiencia de un centro en trasplante hepático en adultos y niños.

Orthotopic liver transplantation (THO) is the treatment of choice for a variety of liver diseases. The national experience before 1993 has been scarce. In November 1993 we started our experience in THO at Clínica Las Condes, as part of a multiorgan transplant program (liver, kidney, pancreas). Until January 1995 we have performed 14 THO in 13 recipients (one retransplantation), of which 5 were in pediatric cases. The recipients range of age fluctuated between 1 and 61 years. In two pediatric cases a liver allograft reduction was performed. Six recipients (46%) required treatment for acute cellular rejection. One recipient had an hepatic artery thrombosis and had to be retransplanted. There was no operative mortality up to 30 days in cases of primary liver transplants. The retransplanted adult recipient, and another pediatric recipient that died from a late recurrence of a hepatoblastoma, accounted for the mortality of this experience. After an average follow up of 10 months, the actual patient survival in 85%. Of the 11 surviving recipients, 9 are in excellent conditions with a very good quality of life. This experience shows that an active liver transplant program in our country can be successful in obtaining results comparable to those published by very experienced foreign transplant centers.

Peritoneal catheter insertion using laparoscopic guidance.

The use of laparoscopic guidance for abdominal puncture and introduction of ventriculoperitoneal shunt peritoneal catheters using a peritoneal split trocar is proposed. The technique permits location of the catheter in the right parietocolic space or its relocation if trapped within the omentum. Cerebrospinal fluid flow is certified. Since the submission of the paper 6 selected cases have been operated upon without complications.