ABSTRACT
BACKGROUND: Post-cholecystectomy bile duct injuries are identified by the onset of jaundice as well as elevated bilirubin and alkaline phosphatase levels during the peri-operative period. It is unknown how serum oxidative stress markers are modified in patients with post-cholecystectomy bile duct injuries. OBJECTIVE: To determine serum oxidative stress marker levels (lipid peroxidation by-products, nitrites/nitrates and total antioxidant capacity) in patients with post-cholecystectomy bile duct injuries. PATIENTS AND METHODS: A prospective, transversal and analytical study was designed with two groups. Group 1: 5 healthy volunteer subjects. Group 2: 52 patients with post-cholecystectomy bile duct injuries (43 female and 9 male). An elective bilio-digestive reconstruction was performed at week 8. The serum oxidative stress marker levels were quantified by colorimetric method. RESULTS: Patients with bile duct injuries had a significant increased serum lipid peroxides (malondialdehyde and 4-hydroxy-alkenals) and nitric oxide metabolites (nitrites/nitrates) levels compared to the control group. In contrast, total antioxidant capacity in patients with bile duct injuries remained similar compared to healthy controls. CONCLUSIONS: The results show that oxidative stress is usually associated to bile duct injury.
Subject(s)
Bile Ducts/injuries , Bile Ducts/metabolism , Cholecystectomy , Oxidative Stress , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Prospective StudiesABSTRACT
Introducción: las lesiones de las vías biliares postcolecistectomíase establecen por la aparición de ictericia, elevación de las bilirrubinasy de la fosfatasa alcalina durante el periodo perioperatorio.Se desconoce cómo se modifican los marcadores de estrésoxidativo en el suero de los pacientes con lesiones de las vías biliarespostcolecistectomía.Objetivo: determinar los marcadores de estrés oxidativo (productosde peroxidación de lípidos, catabolitos del óxido nítrico ycapacidad antioxidante total) en el suero de pacientes con lesionesde las vías biliares.Pacientes y métodos: se realizó un estudio prospectivotransversal analítico. Se formaron 2 grupos de estudio. Grupo 1:5 voluntarios sanos que sirvieron como control. Grupo 2: 52 pacientescon lesiones de las vías biliares postcolecistectomía (43 femeninosy 9 masculinos). La reconstrucción bilio-digestiva se realizóde manera electiva a las 8 semanas. Los niveles séricos de losmarcadores de estrés oxidativo se cuantificaron mediante métodoscolorimétricos.Resultados: los niveles séricos de los productos de peroxidaciónde lípidos (malondialdehído y 4-hidroxialquenos) y de los catabolitosdel óxido nítrico (nitritos/nitratos) aumentaron significativamenteen los pacientes con lesiones de las vías biliares encomparación a los voluntarios sanos. En contraste, la capacidadantioxidante total en el suero de los pacientes con lesiones de lasvías biliares fue similar a la de los voluntarios sanos.Conclusiones: los resultados muestran que el estrés oxidativoen suero es un componente que se asocia a las lesiones de las víasbiliares(AU)
Background: post-cholecystectomy bile duct injuries are identified by the onset of jaundice as well as elevated bilirubin and alkaline phosphatase levels during the peri-operative period. It is unknown how serum oxidative stress markers are modified in patients with post-cholecystectomy bile duct injuries. Objective: to determine serum oxidative stress marker levels (lipid peroxidation by-products, nitrites/nitrates and total antioxidant capacity) in patients with post-cholecystectomy bile duct injuries. Patients and methods: a prospective, transversal and analytical study was designed with two groups. Group 1: 5 healthy volunteer subjects. Group 2: 52 patients with post-cholecystectomy bile duct injuries (43 female and 9 male). An elective bilio-digestive reconstruction was performed at week 8. The serum oxidative stress marker levels were quantified by colorimetric method. Results: patients with bile duct injuries had a significant increased serum lipid peroxides (malondialdehyde and 4-hydroxy-alkenals) and nitric oxide metabolites (nitrites/nitrates) levels compared to the control group. In contrast, total antioxidant capacity in patients with bile duct injuries remained similar compared to healthy controls. Conclusions: the results show that oxidative stress is usually associated to bile duct injury(AU)
