ABSTRACT
OBJECTIVE: To study the effect and underlying mechanisms of Chinese medicine Yanghe decoction on pain relief in a rat model of bone metastasis of breast cancer induced by michigan cancer foundation-7 (MCF-7). METHODS: Bone pain was induced in the tibia of rats injected with MCF-7 cells. The Chinese herbal remedy was used to decoct Yanghe decoction for the treatment of bone pain rats. The behavior study was carried out to evaluate the paw mechanical withdraw threshold and thermal withdraw latency. Liquid chromatography-mass spectrometry, Western blotting, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay (ELISA), immunohistochemical (IHC) staining were performed for analysis. RESULTS: Yanghe decoction could improve the defensive behavior similar to the transient receptor potential ankyrin 1 (TRPA1) inhibitor. In morphology study, Yanghe decoction could attenuate the cellular growth as well as inflammatory infiltration in the metastasis group. Furthermore, Yanghe decoction downregulated the TRPA1 expression on the dorsal root ganglion from the metastatic rats at both transcriptional and protein level. Yanghe decoction alleviated the inflammation in metastatic tissues by hematoxylin-eosin and IHC analysis, and Yanghe decoction also reduced the inflammatory cytokines production in the serum including tumor necrosis factor-α and interleukin-6, interleukin-1 beta by ELISA. As the cytochromec oxidase subunit II/prostaglandin E2 (PGE2) is required for cancer development, Yanghe decoction reduced the expression of PGE2 in the tissue and serum. CONCLUSION: Taken together, Yanghe decoction protected the rats from breast cancer bone metastasis through TRPA1 signaling mediated neuropathic pain and additional immune modulation in tumor microenvironment.
Subject(s)
Ankyrins , Neoplasms , Rats , Animals , Dinoprostone , Michigan , Pain , Tumor MicroenvironmentABSTRACT
OBJECTIVE:To study the effects of Astragali radix injection combined with entecavir on serum inflammatory cyto-kines and liver function of model mice with hepatitis B. METHODS:60 mice were randomly divided into normal control group (normal saline),model group (normal saline),Astragali radix injection group (0.5 mL/10 g),entecavir group (45 μg/kg) and combination group(0.5 mL/10 g Astragali radix injection+45 μg/kg entecavir),12 in each group. Except for normal control group, mice in other 4 groups were induced for hepatitis B models. After modeling,all mice were intragastrically administrated once a day,for 4 weeks. After administration,tumor necrosis factor α(TNF-α),interferon γ(IFN-γ),interleukin 8 (IL-8),aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL)levels in serum were detected,and the pathologi-cal changes in liver tissue in each group were observed. RESULTS:Compared with normal control group,TNF-α,IFN-γ,IL-8, AST,ALT,TBIL levels in serum of mice in model group were significantly increased(P<0.05);and liver cells were diffuse and severe steatosis. Compared with model group,TNF-α,IFN-γ,IL-8,AST,ALT,TBIL levels in serum of mice in each administra-tion group were significantly decreased(P<0.05),pathological changes in liver tissue were improved to varying degrees,and the index improvement degree in combination group was superior to Astragali radix injection group and entecavir group (P<0.05). CONCLUSIONS:Astragali radix injection combined with entecavir helps to down-regulate the expressions of inflammatory cyto-kines of model mice with hepatitis B,and improve the liver function. The combination use has better effect than single use.
ABSTRACT
Objective To analyze the epidemic characteristics of imported malaria in Liyang City,so as to provide the evi?dence for formulating appropriate control strategies and measures. Methods The malaria epidemic situation reports,case epi?demiological investigations and the follow?up materials of counterparts returned together with the cases were collected and ana?lyzed in Liyang City from 2007 to 2014. Results Totally 53 imported malaria cases were reported and they were all laboratory confirmed in Liyang City from 2007 to 2014. Forty ? nine cases(92.5%)were imported from African countries,37 cases (69.8%)were Plasmodium falciparum infection,and 37 cases(69.8%)had overseas incidence history. Forty cases(75.5%) were aged between 30 and 49 years,and 52 cases(98.1%)were male,42 cases(79.2%)were farmers. These cases were dis?tributed in all the 10 towns and there were no obvious seasonal variation for onset time. The median time from onset to seeing a doctor was 3 d,and the median time from seeing a doctor to being diagnosed was 0 d. The first visit health facilities by the pa?tients were various,and the diagnostic health facilities were all the medical institutions at the county level or above,and the standardized treatment rate was 100%. Conclusion During the last 5 consecutive years,there have been no local infected ma?laria cases in Liyang City,and the remarkable results have been achieved in malaria elimination. However,there are still many imported malaria cases,and the infected species are diverse. Therefore,the imported malaria is still the key of malaria preven?tion and control.
ABSTRACT
BACKGROUND:Liushen Pil is a traditional Chinese herbal formula, has the effects of heat-clearing and detoxicating, eliminating stagnation, detumescence and al eviating pain. Modern pharmacology verifies that Liushen Pil has anti-inflammatory, analgesic, cardiac, anti-viral, anti-tumor effects, and has been extensively used in the treatment of various infectious diseases and malignant cancer. OBJECTIVE:To investigate the inhibitory effects of Liushen Pil on esophageal cancer xenografts, and effects on microvessel density and vascular endothelial growth factor expression. METHODS:After reproducing nude mouse models of human esophageal cancer, 48 nude mice were randomly divided into high-dose Liushen Pil group, moderate-dose Liushen Pil group, low-dose Liushen Pil group, cisplatin group, model group and blank group. According to medication regimen, drugs were given. The growth of transplanted tumor of nude mice was dynamical y observed in each group. The nude mice were sacrificed after 20 days of treatment. Neoplasm weight was taken and the tumor-suppressing rate was calculated. Immunohistochemistry was used to detect microvessel density and the expression of vascular endothelial growth factor. RESULTS AND CONCLUSION:The weight of transplanted tumor was significantly lower in the high-dose Liushen Pil group, moderate-dose Liushen Pil group, low-dose Liushen Pil group, and cisplatin group than in the model group (P<0.05). Microvessel density and the expression of vascular endothelial growth factor were obviously lower in the each Liushen Pil group than in the model group, but not as apparent as that in the cisplatin group. Results suggested that Liushen Pil can inhibit the growth of the esophageal cancer xenografts. Liushen Pil can down-regulate the expression of vascular endothelial growth factor and reduce microvessel density, which is one of the tumor-inhibiting mechanism of Liushen Pil .
ABSTRACT
Seven cases of cystic eckinococcosis( CE) were surveyed retrospectively in 2008. The results showed that 6 of thosepatients were locally infected. It is important to investigate the course and characteristics of CE transmission in non-endemic areas.
