ABSTRACT
Four new species of the Ascomycete genus Microglossum are recognized, based on morphological characters and DNA sequences of nuc rDNA (ITS region and 28S gene) and the second largest subunit of RNA polymerase II (RPB2). They differ from Microglossum nudipes by the color of the ascocarps and the sizes and shapes of ascospores, asci, and paraphyses. A lectotype is proposed, and an emended description is provided for M. nudipes. Descriptions of new species Microglossum clavatum, M. truncatum, M. pretense, and M. tenebrosum are provided. Other closely related species in the group of green earth tongues include Microglossum viride, M. rickii, and M. griseoviride. An identification key to green Microglossum species is presented.
Subject(s)
Ascomycota/classification , Ascomycota/genetics , Genetic Variation , Ascomycota/cytology , Cluster Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Phylogeny , RNA Polymerase II/genetics , RNA, Ribosomal, 28S/genetics , Sequence Analysis, DNA , Spores, Fungal/cytologyABSTRACT
Studies in Microglossum viride (Pers.) Gillet revealed that the name was used incorrectly for two similar but different taxa. Analyses of morphological, ecological and molecular (sequences of ITS and LSU region of rRNA gene) characters of type and voucher specimens of M. viride and related taxa resulted in delimitation and description of a new species, Microglossum griseoviride V. Kucera, Lizon & M. Tomsovský. Lectotypes of Geoglossum viride Pers., and epitype of Geoglossum viride are designated. Species Microglossum minus Velen. and Microglossum lutescens Boud. are confirmed to be conspecific to M. viride.
Subject(s)
Ascomycota/classification , Ascomycota/growth & development , Ascomycota/genetics , Ascomycota/isolation & purification , DNA, Fungal/genetics , DNA, Ribosomal/genetics , Molecular Sequence Data , Phylogeny , Soil Microbiology , Spores, Fungal/classification , Spores, Fungal/genetics , Spores, Fungal/growth & development , Spores, Fungal/isolation & purificationABSTRACT
A taxonomic and nomenclatural revision of some representatives of Clavariaceae is presented based on extensive collecting in central and western Europe. Five species originally described from Europe are identified, redescribed and delimited: Clavaria fragilis, Ramariopsis crocea, R. corniculata, R. helvola and R. pulchella. Lectotypes, epitypes or neotypes are designated for all these species. Descriptions are based on macro- and micromorphological characters and supplemented with DNA analyses of the nrLSU regions from 20 specimens. The molecular phylogenetic analyses reconstructed a phylogram showing relationships among the discussed species as well as some closely related taxa. The taxonomic value of the ratio of length and width of spores (Q-value) is discussed.
