ABSTRACT
PurposeTo study the immunohistochemical features of the capsule tissue surrounding MIRAgel episcleral buckles.Patients and methodsThis Institutional interventional clinical cohort study examined a consecutive series of 21 referred patients who required MIRAgel removal from July 2009 to July 2013. All patients with hydrated and fragmented MIRAgel episcleral buckles were included in this study. Capsule biopsies from MIRAgel episcleral buckles were obtained from all patients. Capsule specimens of seven patients with extruded silicone bands were processed as controls. Paraffin-embedded specimens were examined using light microscopy and immunohistochemistry (via the PAP horseradish peroxidase technique) to detect the expression of CD3, CD20, CD34 and CD68, and S-100 protein.ResultsInflammation with granuloma, which was primarily related to sutures, was found in all (n=36) of the MIRAgel specimens and foreign body granulomas with multinucleated giant cells, histiocytes, and macrophages (CD68+ cells) surrounded the MIRAgel fragments. Average number of CD68+ cells was higher (P<0.001) for MIRAgel than for silicone rubber. The lymphocytic inflammatory infiltrate related to the MIRAgel fragments was CD3+ and CD20- (delayed T cell-mediated immune response). Moderate neoangiogenesis was indicated by the presence of CD34+ cells.ConclusionsThe immunohistochemical analysis revealed that the immune system is able to identify the fragments of MIRAgel (after its hydrolytic degradation) as a foreign body during a delayed T cell-mediated immune response. The phagocytosis by macrophages likely triggers and perpetuates local disease. Removal of MIRAgel explants before hydrolysis should be considered.
Subject(s)
Antigens, CD34/metabolism , Antigens, CD/metabolism , Antigens, Differentiation, Myelomonocytic/metabolism , CD3 Complex/metabolism , Eye Foreign Bodies/metabolism , Granuloma, Foreign-Body/metabolism , Polyhydroxyethyl Methacrylate/analogs & derivatives , Scleral Buckling/instrumentation , Device Removal , Humans , Immunoenzyme Techniques , Microscopy , Retinal Detachment/surgery , S100 Proteins/metabolism , Sclera/metabolismABSTRACT
Odontogenic myxoma is a locally aggressive, uncommon benign tumour which arises from mesenchymal tissues normally present in developing teeth. The most frequent locations of odontogenic myxoma are the posterior regions of the mandible, as well as the condylar region. Since odontogenic myxomas are not associated with any specific clinical or radiological sign, a histopathological examination of the specimen is required for confirmation of the primary diagnosis. We report three cases of myxoma diagnosed during the last 18 years. Two of them were located in atypical regions of the mandible and one was located in the maxilla. Presence of a slow-growing swelling associated with expansion of the bone plates raised suspicion of a tumour in two cases, while in the third patient the myxoma was an incidental finding during radiological examination. Due to the unspecific nature of these lesions, in every case a histopathological examination of the surgical specimen was required for diagnostic confirmation. In one of the three reported cases, we shall underline the need to follow a correct diagnostic work-up of all radiolucent lesions of the jaws, in order to avoid contraindicated therapeutic procedures.
Subject(s)
Mandibular Neoplasms/diagnosis , Maxillary Neoplasms/diagnosis , Myxoma/diagnosis , Adult , Diagnosis, Differential , Female , Granuloma, Giant Cell/diagnostic imaging , Humans , Male , Mandibular Diseases/diagnostic imaging , Mandibular Neoplasms/pathology , Maxillary Neoplasms/pathology , Middle Aged , Molar, Third/diagnostic imaging , Myxoma/pathology , Odontogenic Tumors/diagnosis , Odontogenic Tumors/pathology , Radicular Cyst/diagnostic imaging , Radiography , Tooth, Impacted/diagnostic imagingABSTRACT
El mixoma odontogénico es un tumor benigno poco frecuente, con agresividad local, que se origina a partir de los tejidos mesenquimales de los dientes en desarrollo. Su localización preferente son las zonas posteriores de la mandíbula, así como el área condilar. Clínica y radiográficamente no muestra signos de especificidad, por lo que en su diagnóstico es necesario el estudio histopatológico del tejido para confirmar la tumoración.Presentamos tres casos de mixomas recogidos en los últimos 18 años, dos localizados en zonas atípicas mandibulares y uno en la maxilar. La presencia de un crecimiento lento, con expansión de la cortical ósea en do permitió s casos permitio sospechar la presencia del tumor, y el tercer caso constituye un hallazgo casual durante la exploración radiográfica. En todas las ocasiones ha sido necesario el estudio histopatológico de la pieza quirúrgica para confirmar el diagnóstico dada la inespecificida de la lesión; en uno de ellos llamamos la atención sobre la necesidad de realizar un correcto protocolo diagnóstico de las lesiones radiotransparentes de los maxilares, a efectos de evitar procedimientos terapéuticos contraindicados (AU)
Subject(s)
Adult , Female , Male , Middle Aged , Humans , Myxoma/diagnosis , Mandibular Neoplasms/diagnosis , Maxillary Neoplasms/diagnosis , Myxoma/surgery , Maxillary Neoplasms/surgery , Mandibular Neoplasms/surgeryABSTRACT
We have tested de possible protective effects of UVA radiated spleen fragments upon simultaneous skin allografts in 84 rats. Under these circumstances skin graft rejection was less evident with a 20 day graft disappearance rate of 46.87% rate in the treated series, that compares favourably with a 80% in the control series. This study shows a parallel increase in the mean survival time of skin grafts in treated specimens. The figures being 19.8 days in the treated series against 12.9 days in untreated controls. Spleen implants are finally shown to produce a healing stimulus in the skin graft zone. These differences are statistically significant.
Subject(s)
Graft Rejection/prevention & control , Preoperative Care/methods , Skin Transplantation , Spleen/radiation effects , Spleen/transplantation , Ultraviolet Rays , Animals , Cicatrix/pathology , Graft Survival , Male , Rats , Rats, Wistar , Skin/pathology , Wound HealingABSTRACT
A retrospective study of retinoblastoma was done during 1978-1987, analysing the clinics aspects of the entity. We found nineteen children during this period, from them eight were girls (42%) and eleven were boys (58%). Ages of diagnostic ranged between three months and four years, with and average of 17.53 months. Nine children (47%) were bilaterally affected. Leocokoria was the most common initial sign, followed by strabismus. Ophthalmoscopy was the diagnostic procedure used, and surgery the recommended therapeutic, using radiotherapy as a palliative method in the cases which surgery has no indications.
Subject(s)
Eye Neoplasms/diagnosis , Retinoblastoma/diagnosis , Child, Preschool , Eye Neoplasms/epidemiology , Female , Humans , Infant , Male , Retinoblastoma/epidemiology , Retrospective Studies , Spain/epidemiologyABSTRACT
We present a review of the lipomas in the buccal mucosa in relation with our experience in seven cases.
Subject(s)
Lipoma/pathology , Mouth Neoplasms/pathology , Adult , Aged , Female , Humans , Male , Middle AgedABSTRACT
In the present work, the authors show their experience in tongue surgery with Co2 laser. Two different kinds of partial glosectomies were made in several cases, CO2 laser was used on the right side, and conventional methods on the left side as a comparative group. Histological studies were made between both sides, in order to find differences in normal hemostasy and tissue response.
Subject(s)
Glossectomy/methods , Laser Therapy/instrumentation , Animals , Dogs , Glossectomy/instrumentation , Hemostasis , Wound HealingSubject(s)
Endotoxins/pharmacology , Liver/drug effects , Serratia marcescens , Animals , Liver/pathology , Liver/ultrastructure , Male , RatsABSTRACT
The author makes a review of the morphological bases of oncology, considering the methods used in pathological anatomy, the data obtained from histopathology and ultrastructure of neoplasms, and the morphological criteria useful for prognosis.
