Wide-area and omnidirectional optical detector arrays using modular optical elements.

This paper presents novel, modular optical detector arrays of various shapes and configurations. Recently developed Modular Optical Wireless Elements (MOWE) architecture serves as the basis for large and complex optical detector arrays that can be constructed as geometric shells and provide wide-area even omnidirectional field-of-view (FoV). Programmable optical modules synchronously sample the environment, and then route measurements to the user through a dedicated electrical backbone. The arrays are inexpensive, easy to construct, and can be made with homogeneous/inhomogeneous optical properties. Applications include remote sensing, motion detection, optical navigation, and medical imaging, among others. We present the MOWE detector array concept with a detailed optical analysis and a suggested design methodology, as well as a number of various demonstrations. We also utilize wavelength-diversity of MOWE arrays to demodulate two overlapping signals, showing that many diversity-based algorithms can be conveniently prototyped and implemented.

Reengineering a transmembrane protein to treat muscular dystrophy using exon skipping.

Exon skipping uses antisense oligonucleotides as a treatment for genetic diseases. The antisense oligonucleotides used for exon skipping are designed to bypass premature stop codons in the target RNA and restore reading frame disruption. Exon skipping is currently being tested in humans with dystrophin gene mutations who have Duchenne muscular dystrophy. For Duchenne muscular dystrophy, the rationale for exon skipping derived from observations in patients with naturally occurring dystrophin gene mutations that generated internally deleted but partially functional dystrophin proteins. We have now expanded the potential for exon skipping by testing whether an internal, in-frame truncation of a transmembrane protein γ-sarcoglycan is functional. We generated an internally truncated γ-sarcoglycan protein that we have termed Mini-Gamma by deleting a large portion of the extracellular domain. Mini-Gamma provided functional and pathological benefits to correct the loss of γ-sarcoglycan in a Drosophila model, in heterologous cell expression studies, and in transgenic mice lacking γ-sarcoglycan. We generated a cellular model of human muscle disease and showed that multiple exon skipping could be induced in RNA that encodes a mutant human γ-sarcoglycan. Since Mini-Gamma represents removal of 4 of the 7 coding exons in γ-sarcoglycan, this approach provides a viable strategy to treat the majority of patients with γ-sarcoglycan gene mutations.

Design analysis of a fiber-bundle-based mobile free-space optical link with wavelength diversity.

This paper reports on a simulation-based investigation of a wavelength diverse free-space optical link with an emphasis on identifying design choices that optimize the performance of the system under different operating scenarios. The simulation incorporates experimental data into the theoretical calculations for optical propagation to better describe the performance of the physical designs. The performance is evaluated in terms of the coverage area at the receiver, which is a measure of misalignment tolerance and is dependent not only on wavelength but on other key parameters, such as link length, transmitted power, the pattern of transmitters, beam divergence, and the receiver construction. The investigation finds that the coverage area of the receiver can be optimized for a given wavelength by proper choices of these parameters, and that parameter choices exist that minimize the change in performance when switching between wavelengths or for variations in link parameters. The interrelationships among key parameters and their impact on the potential system performance are investigated. The results provide guidance on the further development of the overall system.

Switched diversity approach for multireceiving optical wireless systems.

A number of existing spatial diversity schemes have been shown to improve the performance of optical wireless communication systems in diversity-rich environments. Among all, switched diversity has low complexity and is simple to implement. In this paper, an innovative spatial diversity scheme based on switched diversity is proposed. The scheme, namely switch-to-dominant combining, contributes to a higher bit error rate (BER) performance when compared to conventional switched diversity schemes, including switch-and-stay and switch-and-examine diversity. The optical multireceiver wireless system operates in a spatially correlated and lognormally distributed fading channel. Analytical analyses are conducted to demonstrate BER and processing load performance offered by the new scheme and compare them to available schemes, i.e., conventional switched combining and selection combining.

SMAD signaling drives heart and muscle dysfunction in a Drosophila model of muscular dystrophy.

Loss-of-function mutations in the genes encoding dystrophin and the associated membrane proteins, the sarcoglycans, produce muscular dystrophy and cardiomyopathy. The dystrophin complex provides stability to the plasma membrane of striated muscle during muscle contraction. Increased SMAD signaling due to activation of the transforming growth factor-ß (TGFß) pathway has been described in muscular dystrophy; however, it is not known whether this canonical TGFß signaling is pathogenic in the muscle itself. Drosophila deleted for the γ/δ-sarcoglycan gene (Sgcd) develop progressive muscle and heart dysfunction and serve as a model for the human disorder. We used dad-lacZ flies to demonstrate the signature of TGFß activation in response to exercise-induced injury in Sgcd null flies, finding that those muscle nuclei immediately adjacent to muscle injury demonstrate high-level TGFß signaling. To determine the pathogenic nature of this signaling, we found that partial reduction of the co-SMAD Medea, homologous to SMAD4, or the r-SMAD, Smox, corrected both heart and muscle dysfunction in Sgcd mutants. Reduction in the r-SMAD, MAD, restored muscle function but interestingly not heart function in Sgcd mutants, consistent with a role for activin but not bone morphogenic protein signaling in cardiac dysfunction. Mammalian sarcoglycan null muscle was also found to exhibit exercise-induced SMAD signaling. These data demonstrate that hyperactivation of SMAD signaling occurs in response to repetitive injury in muscle and heart. Reduction of this pathway is sufficient to restore cardiac and muscle function and is therefore a target for therapeutic reduction.

Adaptive divergence and power for improving connectivity in free-space optical mobile networks.

A significant challenge for free-space optical (FSO) links is the restrictive alignment requirements, especially when the transceivers are moving. For moderate distances and rapid unpredictable motion, the receiver's field of view and the positioning system's dynamics become factors. We explore the use of adaptive transmitter power and beam divergence to improve the likelihood of maintaining a mobile FSO link by using Gaussian beam propagation theory and link budgets. We calculate the allowable misalignment between the transceivers' optical axes as a function of power, divergence, and transceiver distance. The maximum allowable error is independent of the distance, except when the field of view is a limiting factor. Certain combinations of divergence and power, while suboptimal for one distance, provide a relaxed misalignment limit for many distances. Based on the calculations, we make initial suggestions for system design.

Characterization of a differential fiber Bragg grating sensor for oil-water boundary detection.

We investigate the operating characteristics of an oil-water boundary detector utilizing differential interrogation of fiber Bragg grating sensors. The system resolution is shown to be stable with respect to the choice of strain actuator and long-term temperature changes and changes in the initial strain on the fiber string. Fluid flow, particularly turbulent flow, is found to reduce system resolution significantly for the current system design. Improvements in the system design are required to minimize the effects of fluid flow and to accurately detect the presence of oil-water emulsions.

Differential strain measurement using multiplexed fiber Bragg grating sensors.

We investigate differential measurement of strain using fiber Bragg gratings and spatial demultiplexing methods. Differential measurement is shown to improve strain resolution in the presence of noise by an average of 34% compared to absolute measurements of the Bragg wavelengths. Spatial demultiplexing of the gratings provides potentially high-resolution sensing without calculating the sensor wavelengths. The differential method is still temperature dependent. We apply the differential technique to the problem of oil-water boundary detection in production columns, with promising preliminary results.

Wavelength and intensity dependence of retinal evoked responses using in vivo optic nerve recording.

We use a whole-animal model and wavelength-selective optical stimulation to investigate relationships between optical stimulus characteristics and neural signaling. Light-emitting diodes are used to selectively stimulate rod and cone pathways in Rana pipiens. A suction electrode is used to make in vivo measurements of the compound action potential from the optic nerve as the wavelength and intensity of the stimulus is varied. Our results demonstrate that the cone and rod pathways can be separately stimulated and analyzed with our method and, thus, provide a means to model the response of such pathways to more complex stimuli.