The Bloody Crossroads: Interactions between Periodontitis and Hematologic Diseases.

Periodontitis is a common oral condition that can have a significant impact on the overall health of the body. In recent years, attention has been paid to potential relationships between periodontitis and various hematological disorders. This publication aims to present information available in the literature on this relationship, focusing on examples of red blood cell disorders (such as aplastic anemia and sickle cell anemia) and white blood cell disorders (such as cyclic neutropenia, maladaptive trained immunity, clonal hematopoiesis, leukemia, and multiple myeloma). Understanding these associations can help physicians and dentists better diagnose, monitor, and treat patients associated with both groups of conditions, highlighting the need for interdisciplinary care for patients with oral disorders and hematologic diseases.

How Can Imbalance in Oral Microbiota and Immune Response Lead to Dental Implant Problems?

Dental implantology is one of the most dynamically developing fields of dentistry, which, despite developing clinical knowledge and new technologies, is still associated with many complications that may lead to the loss of the implant or the development of the disease, including peri-implantitis. One of the reasons for this condition may be the fact that dental implants cannot yield a proper osseointegration process due to the development of oral microbiota dysbiosis and the accompanying inflammation caused by immunological imbalance. This study aims to present current knowledge as to the impact of oral microflora dysbiosis and deregulation of the immune system on the course of failures observed in dental implantology. Evidence points to a strong correlation between these biological disturbances and implant complications, often stemming from improper osseointegration, pathogenic biofilms on implants, as well as an exacerbated inflammatory response. Technological enhancements in implant design may mitigate pathogen colonization and inflammation, underscoring implant success rates.

Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?

The matter constituting the enamels of four types of organisms was studied. The variability of the ions was presented in molar units. It was proven that the changes in water contents of the enamel are significantly positively related to changes in Mg; inversely, there is also a strong connection with changes in Ca and P, the main components of bioapatite. The variability in the organic matter has the same strong and positive characteristics and is also coupled with changes in Mg contents. Amelogenins in organic matter, which synthesize enamel rods, likely have a role in adjusting the amount of Mg, thus establishing the amount of organic matter and water in the whole enamel; this adjustment occurs through an unknown mechanism. Ca, P, Mg, and Cl ions, as well as organic matter and water, participate in the main circulation cycle of bioapatites. The selection of variations in the composition of bioapatite occurs only along particular trajectories, where the energy of transformation linearly depends on the following factors: changes in the crystallographic d parameter; the increase in the volume, V, of the crystallographic cell; the momentum transfer, which is indirectly expressed by ΔsinΘ value. To our knowledge, these findings are novel in the literature. The obtained results indicate the different chemical and crystallographic affinities of the enamels of selected animals to the human ones. This is essential when animal bioapatites are transformed into dentistic or medical substitutes for the hard tissues. Moreover, the role of Mg is shown to control the amount of water in the apatite and in detecting organic matter in the enamels.

Evaluation of the Tooth Surface after Irradiation with Diode Laser Applied for Removal of Dental Microorganisms from Teeth of Patients with Gingivitis, Using X-ray Photoelectron (XPS) and Optical Profilometry (OP).

Gingivitis is accompanied by microorganisms, including pathogens, which must be eliminated to speed up the treatment of inflammation. Laser irradiation may be one of the safe methods for reducing tissue contamination on the tooth surface. The aim of the study was the assessment of the tooth surface in patients with gingivitis after the use of a diode laser to eliminate microorganisms living there. In the first stage of the research, microorganisms were isolated (Candida albicans, C. guilliermondii, Escherichia coli, Haemophilus parainfluenzae, Klebsiella oxytoca, Neisseria subflava, Rothia dentocariosa, Rothia mucilaginosa, Streptococcus pneumoniae) from three patients with gingivitis, their identification confirmed using the MALDI-TOF MS technique (matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry). Then, the irradiation process with a diode laser was optimized to a wavelength of 810 nm ± 10 nm in five variants to reduce microorganisms on the tooth. The tooth surface was analyzed by X-ray photoelectron spectroscopy (XPS) and optical profilometry (OP) before and after irradiation. 103 to 106 CFU were detected on a 0.4 cm2 tooth area. Nine types of bacteria and two types of fungi dominated among the microorganisms. The laser at the most effective biocidal dose of 25 W/15.000 Hz/10 µs, average = 3.84 W, with three uses after 15 s, increased the reduction of fungi from 57.97% to 93.80%, and bacteria from 30.67% to 100%. This dose also caused a decrease in the degree of oxidation and in the effect of smoothing on the treated surfaces.

Analysis of the Microbiome on the Surface of Corroded Titanium Dental Implants in Patients with Periimplantitis and Diode Laser Irradiation as an Aid in the Implant Prosthetic Treatment: An Ex Vivo Study.

The paper presents the optimization of diode laser irradiation of corroded dental implants in order to reduce the number of microorganisms associated peri-implantitis. The research included the identification of microorganisms on the surface of removed dental implants in patients with peri-implantitis and the assessment of the biocidal effectiveness of the diode laser against these microorganisms. Laser desorption/mass spectrometry (MALDI-TOF MS) was used to identify microorganisms and metagens were examined by next generation sequencing (NGS). Irradiation was performed with a diode laser with a wavelength of λ = 810, operating mode: 25 W/15.000 Hz/10 µs, average = 3.84 W with the number of repetitions t = 2 × 15 s and t = 3 × 15 s. The structure and surface roughness of the implants were analysed before and after laser irradiation by optical profilometry and optical microscopy with confocal fixation. In total, 16 species of Gram-positive bacteria and 23 species of Gram-negative bacteria were identified on the surface of the implants. A total of 25 species of anaerobic bacteria and 12 species with corrosive potential were detected. After diode laser irradiation, the reduction in bacteria on the implants ranged from 88.85% to 100%, and the reduction in fungi from 87.75% to 96.77%. The reduction in microorganisms in the abutment was greater than in the endosseous fixture. The applied laser doses did not damage, but only cleaned the surface of the titanium implants. After 8 years of embedding, the removed titanium implant showed greater roughness than the 25-year-old implant, which was not exposed to direct influence of the oral cavity environment. The use of a diode laser in an optimised irradiation dose safely reduces the number of microorganisms identified on corroded dental implants in patients with peri-implantitis.

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies.

Patients undergoing implant treatment are at risk of peri-implant bone loss, which is most often caused by the adverse effects of microorganisms, but there are few proven procedures for their reduction. The aim of the research was to identify the microorganisms inhabiting the composites used to close the screw access hole (SAH), compare them numerically with those present on the surface of crowns and teeth, and optimize the doses of the diode laser, which will reduce microorganisms and will not deteriorate the roughness of polished composites. Patients were swabbed from the surface of SAH composites, from porcelain and zirconium restorations, and from teeth, and then the number of microorganisms was determined by using a culture technique. Microorganisms were identified by MALDI-TOF MS and NGS sequencing. The effectiveness of diode laser irradiation was achieved by using four variants of exposure. After polishing and laser irradiation, the surface roughness of the composites was studied by using optical profilometry. On the surface of SAH, 106 to 108 microorganisms were identified at 0.4 cm2, including many pathogenic species. Among the materials used for the reconstruction of dental implants, the greatest microbiological contamination was found on the composites used to close the SAH. The diode laser with a wavelength of 810 nm with an average power of 3.84 W, during 60 s and 2 × 30 s, has a biocidal effect and does not significantly change the surface roughness of composites. The best reduction of microorganisms was achieved on a composite polished with a polishing rubber and then with a Sof-Lex™ Pre-Polishing Spiral beige (3M ESPE, Ave. St. Paul., MN, USA). Studies have shown that using the optimal laser dose can help treat periimplantitis. These studies provide important information on the possibility of the effective elimination of microorganisms by using a diode laser in the treatment of peri-implant bone loss.

The Efficacy of a Diode Laser on Titanium Implants for the Reduction of Microorganisms That Cause Periimplantitis.

The paper presents the optimisation of a safe diode laser irradiation process applied to the surface of titanium implants in order to reduce microbial numbers in the treatment of inflammation classified as periimplantitis. The study comprised isolation and identification of microorganisms inhabiting surfaces of dental implants, crowns, teeth and saliva from patients with fully symptomatic periimplantitis. Microorganisms were detected by a culture-dependent method and identified with the use of MALDI-TOF mass spectrometry. The isolated microorganisms were inoculated on the surface of a new implant and then irradiated by a diode laser (wavelength of 810 ± 10 nm) in one, two or three repetitions and biocidal efficacy was assessed. To evaluate impact of laser irradiation on roughness, morphology and structure of the implant surface, optical profilometry, scanning electron microscopy and optical microscopy were used. Examination of the tested surfaces and saliva revealed the presence of Gram-positive and Gram-negative bacteria and one fungal species. In all patients, cultures from the endosseous part of the implant revealed the presence of the pathogenic and pyogenic bacterium Streptococcus constellatus. In 13 out of 20 samples laser-irradiated in duplicate and triplicate, all microorganisms were eliminated. The irradiation used did not cause any changes in the properties of the implant surface.

The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants.

The aim of this paper was to study the effectiveness of a diode laser (LD) for removal of microorganisms isolated from porcelain and zirconia crown surfaces used in implantoprosthetics in order to minimize infections around dental implants. In order to optimize biocidal efficacy of the process (at the same time, avoiding increasing the surface roughness during decontamination) the effects of diode laser doses were investigated. The irradiation was performed with a diode laser at the wavelength of λ = 810 nm in three variants with a different number of repetitions (1 × 15 s, 2 × 15 s, 3 × 15 s). The quantitative microbial contamination of the surface of teeth, porcelain and zirconia crowns assessment was made using the culture-dependent method. The identification of microorganisms took place using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and next-generation sequencing (NGS) methods. The studies of the surface morphology and roughness were carried out by means of the optical profilometry, scanning electron microscopy (SEM) and optical microscopy with the C1 confocal attachment. The most important conclusion from the research is the fact that the laser operation, regardless of the exposure time, effectively eliminates the microorganisms from the surfaces used for dental implant rebuilding and does not have a destructive effect on the tested material.

Effective microbiological decontamination of dental healing abutments colonised with Rothia aeria by a diode laser as a helpful step towards successful implantoprosthetic therapy.

The aim of the study was to find variant of diode laser (λ = 810 nm) irradiation, which ensures elimination of unwanted microorganisms, including Rothia aeria, from dental healing abutments, and consequently accelerates process of wound healing in implantologically treated patients. The scope of the study included identification of the most contaminated areas on healing abutments, identification of microorganisms inhabiting various environments of oral cavities, assessment of effectiveness of various laser decontamination parameters against detected microorganisms (preliminary studies) and assessment of wound healing in patients after applying abutments with low roughness and optimal variant of laser irradiation (clinical studies). Imaging of surfaces of the healing abutments was performed using vertical scanning interferometry, scanning electron microscopy and optical microscopy. Microorganisms inhabiting the healing abutments, teeth and saliva from tested patients were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Three programmes of near-infrared diode laser at average powers of 1.00-3.84 W with two variants of exposure time were used for optimisation of laser parameters. Observation of wound healing was performed for 100 patients during 20 days after installation of abutments. On surfaces of the used healing abutments, a large number of microorganisms, with a predominance of R. aeria, were found. Irradiation with periimplantitis surgical programme for 30 s resulted in 99-100% reduction in the number of R. aeria and other microorganisms, depending on type of abutment (in vivo). The use of diode laser in the selected variant accelerates wound healing and provides complete elimination of pathogenic R. aeria and other microorganisms inhabiting surfaces of the healing abutments without marks.

Decontamination of microbiologically contaminated abiotic porous surfaces in an oral surgery clinic using vaporised hydrogen peroxide (VHP).

PURPOSE: The aims of the study were to identify microorganisms, including those in the VBNC state, inhabiting porous surfaces in oral surgery offices and to assess the biocidal effectiveness and impact of 300 ppm vaporised hydrogen peroxide (VHP) for 20 min on decontaminated materials. METHODS: From the surfaces of textured armrests of dental chairs, pinewood doors and window frames and cotton medical aprons, 30 swabs were taken with moistened sponges. The identification of isolated microorganisms was performed using molecular methods with MALDI-TOF MS, DNA Sanger sequencer and Illumina MiSeq. To evaluate the impact of VHP decontamination (independent variable) on the number of microorganisms (response variable) ANOVA and LSD tests were used. After application of 10 processes of VHP decontamination, changes in the properties of the materials were assessed using FTIR spectroscopy, SEM microscopy and XPS spectrometry. RESULTS: The concentration of microorganisms was 101-104 CFU/100 cm2 on the tested surfaces and 102 CFU/m3 in the air. Twenty species of bacteria, one yeast and 16 filamentous fungi were identified, with the predominance of Bacillus, Staphylococcus, Alternaria, Aspergillus and Penicillium. Moreover, Janthinobacterium, Acremonium, Aureobasidium, Coprinellus and Cosmospora in the VBNC state were metagenomically detected. VHP decontamination resulted in a reduction in the majority of tested microbial strains by a minimum of 3 log, and all tested mixed cultures inhabiting porous surfaces were above 98% and in the air, 100%. VHP decontamination did not affect the structural and morphological properties of cotton fibres, wood or stainless steel. CONCLUSIONS: VHP decontamination at a concentration of 300 ppm for 20 min can be used for the holistic disinfection of air, surfaces and equipment in oral surgery offices.

Prevention of SARS-COV-2 coronavirus spread at the Auschwitz-Birkenau State Museum in Poland. The most visited Memorial site in the world during the COVID-19 pandemic.

INTRODUCTION: At the Auschwitz-Birkenau State Museum (A-BSM) actions have been undertaken to effectively protect employees and minimise risk of SARS-CoV-2 coronavirus spreading from the beginning of the COVID-19 epidemic. AIM OF THE ARTICLE: The aim was to present the actions, instructions and procedures introduced at the A-BSM to provide information how to deal with pandemic caused by the SARS-CoV-2 coronavirus in institutions taking care of cultural heritage before and after closure of the Museum for visitors and after reopening. MATERIALS AND METHODS: The described activities were developed at the Museum by a specially established Expert Team. RESULTS: Groups of employees and places in which they were most exposed to contact with visitors and, as a consequence, at the highest risk of getting infected, were characterised. The employees were provided with personal protective equipment, and at the Museum site, devices essential for maintaining the microbiological cleanliness of the rooms, were placed. In the next stage, instructions and procedures for particular groups of employees were prepared. Visitors were educated about the need of taking precautions and were allowed to disinfect in several places at the Memorial Site. Steps to reduce attendance at this time has also been taken. Procedures for employees of the A-BSM were developed in such way that they can also be used after reopening of the Museum for visitors. CONCLUSIONS: The actions which ensured the microbiological safety of the A-BSM employees, brought satisfying results. In more than 21 days after closing of the Museum for visitors, no cases of SARS-CoV-2 infection or COVID-19 were found among employees, despite a very high threat.

Effect of prophylactic actions on the state of oral health and hygienic habits in a selected age group.

INTRODUCTION AND OBJECTIVE: The aim of the study was investigation of the effect of simple prophylactic methods on the motivation for concern about oral hygiene among 13-15-year-olds. MATERIAL AND METHODS: The study covered 98 children from 4 groups attending junior high school. Adolescents from 3 groups were randomly qualified for conducting prophylactic procedures, while the fourth group constituted the control group. Three examinations were performed: preliminary and check-up examinations after 4 and 12 months. The state of the teeth and periodontium and the frequency of brushing of teeth were evaluated. RESULTS: After 4 months, an increase in the number of decayed teeth was observed in all groups; in the control group it was significantly higher compared to the groups subjected to prophylactic actions. After 4 months in the groups covered by prophylactic actions, a significant decrease was obtained in the number of adolescents with CPITN > 0. In the examinations performed after 12 months, no significant differences were found in the value of the DMF and the CPITN index in the examined groups. In both examinations, after 4 and 12 months, the declared frequency of brushing teeth increased, especially in the group which had been provided individual instructions concerning oral hygiene. CONCLUSIONS: 1) Implementation of prophylactic methods resulted in an improvement in the state of oral health among junior high school adolescents. 2) Prophylactic actions should be regularly repeated. 3) The provision of individual instruction with supervised individual teaching of brushing teeth resulted in an improvement in the motivation for concern about oral hygiene.

Association between clinical stage of oral cancer and expression of immunohistochemical markers.

AIM: The aim of this study is to demonstrate the possible correlation between the expression of examined protein markers - p53, EGFR, PCNA, p44/42 in the mass of the tumor and the clinical stage of disease. MATERIAL: 48 patients of the Department and Clinic of Oral and Maxillofacial Surgery, Lublin diagnosed with oral cancer. The control group consisted of 10 patients diagnosed with leukoplakia lesions in the oral cavity. The methods: Immunohistochemical analysis using the detection system DAKO K5007 Cat - Dako REAL ™ Detection System, Peroxidase DAB +, Rabbit / Mouse. RESULTS: Based upon the statistical results, significant correlation between p53 protein and tumor staging; however, a correlation between the level of expression of EGFR, p44/42, PCNA and staging was not likewise revealed. CONCLUSIONS: Looking for oral squamous cell carcinoma markers remains an actual issue. Identification of specific markers of oral cancer could be used in screening the population, determining prognosis and response to treatment.

Pleomorphic adenoma of the palate: a case report and review of the literature.

Salivary gland tumors are rare and account for 2-3% of tumors occurring in the head and neck. Pleomorphic adenoma is a benign neoplasm which is commonly encountered in the parotid gland and other major salivary glands. At times they can also develop in minor salivary glands of the palate. The majority of minor salivary gland tumors are malignant. This case report describes a case of mixed tumor in a minor salivary gland of the hard palate.