ABSTRACT
Morphological, functional signs of myocardium affection and tolerance to physical loading in animals with isoproterenol-induced affection of myocardium after transplantation of a nuclear-containing cells (NCC) of umbilical blood were analyzed in comparison with a natural course of the model. There was proved, that a transplantation of a NCC promotes acceleration of processes of regeneration and restoration of the damaged myocardium in experimental animals. There were estimated the changes in localization of the transplanted NCC in the zone of damage, and capacity of the peripheral blood NCC, while they were transplanted, using intravenous injection, to migrate into the damage zone. The experimental data obtained need further complex analysis and permit to wait a positive effect while performing clinical investigations in the patients, suffering chronic diseases of the heart.
Subject(s)
Cord Blood Stem Cell Transplantation , Fetal Blood/cytology , Myocardial Infarction/therapy , Myocardium/pathology , Myocytes, Cardiac/pathology , Animals , Cryopreservation , Female , Fetal Blood/physiology , Fetal Blood/transplantation , Humans , Isoproterenol , Mice , Myocardial Infarction/chemically induced , Myocardial Infarction/pathology , Regeneration/physiology , Transplantation, HeterologousABSTRACT
In this paper we show presents of viable population of hepatoblasts, endodermal blasts, endothelial and mesenchymal cells in the cryopreserved suspension cells of human fetal liver. Also we observed epithelial-mesenchymal transition of hepatoblasts in culture. We show that it's possible to apply the method of cryopreservation of hematopoietic cells of human fetal liver of the first gestation trimester for cryopreservation of parenchymal and stromal cells of fetal liver.
