ABSTRACT
Prophyrin biosynthesis from 4,5-dioxovaleric acid was studied in cell suspensions of R. spheroides. The experiments show that 4,5-dioxovaleric acid is a far precursor of porphyrins through delta amino laevulinic acid formation in a transmination reaction involving also l-alanine. It differs radically from the classical delta aminolaevulinic acid synthesis using glycine and succinyl CoA as substrates.
Subject(s)
Porphyrins/biosynthesis , Rhodopseudomonas/metabolism , Alanine/metabolism , Aminolevulinic Acid/metabolism , Valerates/metabolismSubject(s)
18-Hydroxycorticosterone , Corticosterone , Butanols , Chemical Phenomena , Chemistry , Corticosterone/analogs & derivatives , Ethanol , Hydrochloric Acid , Mass Spectrometry , Methanol , WaterABSTRACT
Incorporation of delta-aminolaevulinic acid 5 14C and 4 14C into the inosine monophosphate pool and into porphyrins, was studied in cell suspensions of R. spheroides. The results contradict a direct incorporation of delta-aminolaevulinic acid into the purine ring of nucleotides through gammadelta-dioxovaleric acid. It would suggest a nonspecific incorporation after degradation of delta-aminolaevulinic acid without a transamination as a first reaction.
Subject(s)
Aminolevulinic Acid/metabolism , Levulinic Acids/metabolism , Rhodobacter sphaeroides/metabolism , Inosine Monophosphate/biosynthesis , Porphyrins/biosynthesisABSTRACT
Corticosterone (B) and a less polar form or derivative of 18-hydroxycorticosterone obtained by dissolving the mother-steroid in 0.01 N HCl, (L), were assayed as precursors to aldosterone in: 1) samples only containing buffer (C); 2) samples containing buffer and boiled adrenal tissue (phi); 3) samples containing surviving quartered rat adrenals (G). The yields of aldosterone from B were the following: 1.24% in sample (C); 0.21% in sample pHI; and 5.03% in sample (G). The yields of aldosterone from L were the following: 2.66 % in sample (C); 2.30% in sample (phi) ; and 2.47% in sample (G). A factor inhibiting the conversion of B, but not of L, to aldosterone was present in samples containing boiled tissue.
Subject(s)
18-Hydroxycorticosterone/metabolism , Adrenal Glands/metabolism , Aldosterone/biosynthesis , Corticosterone/analogs & derivatives , Corticosterone/metabolism , Animals , Male , RatsABSTRACT
Corticosterone (B) and a less polar form or derivative of 18-hydroxycorticosterone obtained by dissolving the mother-steroid in 0.01 N HCl, (L), were assayed as precursors to aldosterone in: 1) samples only containing buffer (C); 2) samples containing buffer and boiled adrenal tissue (phi); 3) samples containing surviving quartered rat adrenals (G). The yields of aldosterone from B were the following: 1.24
in sample (C); 0.21
in sample pHI; and 5.03
in sample (G). The yields of aldosterone from L were the following: 2.66
in sample (C); 2.30
in sample (phi) ; and 2.47
in sample (G). A factor inhibiting the conversion of B, but not of L, to aldosterone was present in samples containing boiled tissue.
ABSTRACT
Corticosterone (B) and a less polar form or derivative of 18-hydroxycorticosterone obtained by dissolving the mother-steroid in 0.01 N HCl, (L), were assayed as precursors to aldosterone in: 1) samples only containing buffer (C); 2) samples containing buffer and boiled adrenal tissue (phi); 3) samples containing surviving quartered rat adrenals (G). The yields of aldosterone from B were the following: 1.24
in sample pHI; and 5.03
in sample (G). The yields of aldosterone from L were the following: 2.66
in sample (phi) ; and 2.47
in sample (G). A factor inhibiting the conversion of B, but not of L, to aldosterone was present in samples containing boiled tissue.
